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OfflineMushroomFriend
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Myc in Karo
    #3441870 - 12/03/04 01:16 PM (12 years, 5 months ago)

Ho Ho Ho! :lol:

Here I am again ow what a postwhore I am becoming! :p

Anyways, a Q bout Karo.

3 jars of mine show what I think is growth. BUT it is not that nice big fluffy white ball I see in teh fancy pics here posted by Agar (discman!) and others.

It is like a growing layer of small white fluffya flakes on the bottom. When i gently shake it it is like snowing in the karo.

Can this be Myc or might it be some kind of a contamination?
I did use a small piece of myc-cake in one, and in 2 of them I put some myc cornkernals.

Any comments?


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Offlinejonas
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Re: Myc in Karo [Re: MushroomFriend]
    #3441937 - 12/03/04 01:30 PM (12 years, 5 months ago)

give it a couple days and see where it goes. Also, smell it. Let us know how it smells.


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Offlinespliffmasta
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Re: Myc in Karo [Re: jonas]
    #3441964 - 12/03/04 01:35 PM (12 years, 5 months ago)

MF, I used one of my more flakey cultures a few days ago just so I could answer your question. And it is colonizing so far like normal.


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Offlinedjred
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Re: Myc in Karo [Re: spliffmasta]
    #3441987 - 12/03/04 01:38 PM (12 years, 5 months ago)

yeah mushroom mine is like flakey type myc..didn't really take off beyond that.. i have 2 jars like that its been 2 weeks so i started a new jar hopefully its works out better.


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Offlinespliffmasta
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Re: Myc in Karo [Re: djred]
    #3442002 - 12/03/04 01:39 PM (12 years, 5 months ago)

Yea, I would still look into your techniques because they definitely aren't ideal in that form, and like red said, they don't really progress beyond the initial flakes like my good cultures.


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OfflineMushroomFriend
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Re: Myc in Karo [Re: spliffmasta]
    #3442096 - 12/03/04 02:02 PM (12 years, 5 months ago)

Technique:

- 125 ML JAR
- Lid pierced with screwdriver
- Filled with polyfill
- Solution of 1 TSP Karo @ 100ML water
- Stir it
- put in jar
- lid on
- 5-10 minutes in PC

- PC steam off
- take jars out
- let cool
- take jars to preheated and then cooled down oven(bout 90-100F)
- corn jar in oven
- loosen lids
- open corn jar and take out 2 kernels real quick with a "pincet"
- open karo lid, insert kernels, close lid.
- store in incubator
- look almost every day
- temp between 81-87

thats it

The cake jar I did in open air in the kitchen, not in the oven. Cleaned before with bleech alcohol and H2O2.

NOTE: I use not the karo brand, it is not available here. I use what is called Corn/Barley maltsyrup bought in eko store without additives.


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Offlinekrazycure
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Re: Myc in Karo [Re: MushroomFriend]
    #3442213 - 12/03/04 02:40 PM (12 years, 5 months ago)

Even though Nano's (the starter of the Karo tek over at the nook) recipe called for one teaspoon of karo per 100ML, I've seen people on this board and even myself experiencing better results with 1 TABLEspoon or more. More nutes to devour = more probability for germination between two spores = faster growth = more usable culture. The final DENSITY of your culture depends on the intial karo content.

Also, remember, karo syrup is thick and you REALLY have to stir well to get it evenly distributed... you wouldn't want karo on one side of the jar and just water on the other.

Ideally, you'll get the puff-balls of myc in the final product(expanding in all directions because nutes are in all directions), not flakes... flakes = myc reaching out for more nutes but not finding any in it's immediate vicinity. Myc, especially in karo because it's suspended, needs a solid pathway of nutes to grow along.

Try adding up the total amount of solution you'll need for all our karo jars, and then mixing it bulk in a blender.


Edited by krazycure (12/03/04 02:49 PM)


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OfflineMushroomFriend
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Re: Myc in Karo [Re: krazycure]
    #3442284 - 12/03/04 03:07 PM (12 years, 5 months ago)

Helpfull post crazy, very. Have been thinking of that, adding more syrup .

I stirred very well, since I used warm water to solve the karo in, it was rather easy to do.

So you are saying that I should put the karo liquids together.. Hmmz its to strains, maybe hard to do that sterile.

Shall this wizard do the great karo to karo transfer! ?:D

EDIT:

What exactly is meant by the "final density" part in your post? Meaning the stronger myc you put in the Karo or the MORE myc you put in the karo? Or is it also the NUTE THING? :smile:

I have one jar which I knocked up with 2CC sporesyringe (GT) but same there, only small tiny snowflakes. Less then in the jars where I added corn or cake.

Thanks a lot for your posting.


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Edited by MushroomFriend (12/03/04 03:11 PM)


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Offlinespliffmasta
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Re: Myc in Karo [Re: MushroomFriend]
    #3442346 - 12/03/04 03:30 PM (12 years, 5 months ago)

I have some suggestions for you to give a shot MF:

1) try boiling your jars for 25 minutes rather than messing with the PC, my most successful culture actually came out of my microwave.

2) stir in you karo extremely well before sterilizing

3) use TABLEspoon per 100 mL. I've ran experiments on the content of honey vs. the colonization time, and in every instance the more honey/nutes, the earlier the myc got a good hold, and the faster it colonized overall.


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OfflineMushroomFriend
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Re: Myc in Karo [Re: krazycure]
    #3442349 - 12/03/04 03:31 PM (12 years, 5 months ago)

Ok what is a good way?

This is my plan!

- half pint jar
- 100ML warm water
- 2 tablespoons Cornsyrup
- stir / mix well
- Lid with polyfill again (or should I leave it without a hole?)
- PC it 10 minutes
- let cool

ready

no just kiddin
- prepare oven or flowhood or whatever
- take karo jar with myc-flakes in it, wipe outside real good with cotton drenched in alcohol
- take cooled pint jar
- both in oven
- loosen lids
- poor the "old" karo real quick in new pint jar
- screw lid on
- incubate
- celebrate that I am a born karohero :lol:

Way to go?


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OfflineMushroomFriend
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Re: Myc in Karo [Re: spliffmasta]
    #3442368 - 12/03/04 03:35 PM (12 years, 5 months ago)

Yes me gonna raise the nutes with 100%, I was a bit to carefull with them I think.

Actually someone (dobinky or lemon_w) told me to use the microwave, but im not a fan of the microwave... Gonna try it though.

Actually I did the boil thing, the nasty part is that water can come in through the hole in the lid. I actually wonder how necesarry that is.

I will stir even better then I already did!!! :P

O yes I will also filter it (coffee filter) before sterilizing, that was Agars tip!

Thanks dudes! Any comments on my plan spliffy?


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OfflineMushroomFriend
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Re: Myc in Karo [Re: jonas]
    #3442370 - 12/03/04 03:36 PM (12 years, 5 months ago)

dont wanna open the jars!


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OfflineMushroomFriend
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Re: Myc in Karo [Re: MushroomFriend]
    #3442634 - 12/03/04 05:01 PM (12 years, 5 months ago)

BUMP

My Karo 2 Karo Plan

- half pint jar
- 100ML warm water
- 2 tablespoons Cornsyrup
- stir / mix well
- Lid with polyfill again (or should I leave it without a hole?)
- PC it 10 minutes
- let cool

READY!

no just kiddin :wink:

- prepare oven or flowhood or whatever
- take karo jar with myc-flakes in it, wipe outside real good with cotton drenched in alcohol
- take cooled pint jar
- both in oven
- loosen lids
- poor the "old" karo (100 ML)real quick in new pint jar
- screw lid on
- incubate
- celebrate that I am a born karohero? :lol:

Way to go? 


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Edited by MushroomFriend (12/03/04 05:03 PM)


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Offlinekrazycure
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Re: Myc in Karo [Re: MushroomFriend]
    #3442651 - 12/03/04 05:05 PM (12 years, 5 months ago)

So you are saying that I should put the karo liquids together.. Hmmz its to strains, maybe hard to do that sterile.

No no, I meant, for instance: if you need 3 jars, measure out 300ML of water, pour in blender, then add 3 tablespoons karo, blend, and pour into jars. That's just what I do. Making them individually and manually is fine too I suppose, I just like the idea of it being mechanically blended for some reason.

Shall this wizard do the great karo to karo transfer! ?:D

You can, but it's not recommended. I'd start with fresh spores everytime, as myc gets "tired" after a few transfers... especially if it's already devoured the current available nutes before transfer, i.e., the growth has already stagnated. Your new plan will work if done sterile and right, but I'd start fresh. BTW, about your water coming through the hole issue: "invest" in tyvek.

What exactly is meant by the "final density" part in your post? Meaning the stronger myc you put in the Karo or the MORE myc you put in the karo? Or is it also the NUTE THING?

I meant that the actual thickness and veracity of your finished (ready to use) mycellium culture will depend on the amount of nutes you originally place in the solution. I.e., it will be able to grow for longer periods of time and thus thicker and bigger with more karo syrup.


Edited by krazycure (12/03/04 05:12 PM)


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OfflineMushroomFriend
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Re: Myc in Karo [Re: krazycure]
    #3442755 - 12/03/04 05:26 PM (12 years, 5 months ago)

Thanks very much krazycure.

But the karo I have now if I must choose toss it or try some transfer I'd pick the last.
2 jars are a week old, and the corn karo is from yesterday so might be worth a try?

Or should I try to inoc some substrate with the week old flakies? :smile:


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Offlinespliffmasta
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Re: Myc in Karo [Re: MushroomFriend]
    #3442933 - 12/03/04 06:04 PM (12 years, 5 months ago)

Lose some of your water in the flakey culture, just dump it in a spare glass or something, then pour the rest of the flakey culture in there.


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