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Offlinejonas
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incubation question
    #3435718 - 12/02/04 02:21 AM (12 years, 10 days ago)

I was just wondering about something and I wanted to ask you guys ... I have my incubation chamber set up so that theres enough water in a rubbermaid to go about 3/4 the way up my jars, and i just have them sitting in the water, with a heater set to 86 degrees. Theoretically, wouldn't this actually be better than TiT because the water is at a constant 86 degrees, and its always completely encircling the jars?


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3435799 - 12/02/04 02:38 AM (12 years, 10 days ago)

tit temp can be just as constant ofcourse when heated correct.
in tit is less moist, moist = contam.

but your way is described in the MMGG so it will work.


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Offlinejonas
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Re: incubation question [Re: MushroomFriend]
    #3435813 - 12/02/04 02:41 AM (12 years, 10 days ago)

Thats good to hear, thanks man. And no moisture should really be leaking out of my jars because they're covered with aluminum foil. I have tyvek on them but it left too much surface area exposed for moisture loss so I covered em up. So basically no moisture should come in or out of the jars, even though as you say it creates ALOT of condensation, but I dont anticipate problems.


Edited by jonas (12/02/04 02:42 AM)


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3435830 - 12/02/04 02:46 AM (12 years, 10 days ago)

I think ull be safe with tyvek since its waterproof also.
when colonisation takes off you might wana remove the alufoil so air/gas exchange will be better.


MMGG


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Offlinejonas
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Re: incubation question [Re: MushroomFriend]
    #3435840 - 12/02/04 02:49 AM (12 years, 10 days ago)

Aight, sounds good man. Thanks a bunch.


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3435851 - 12/02/04 02:54 AM (12 years, 10 days ago)

no problem fellow, btw are you swedish?


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Offlinelaughingbuddha
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Re: incubation question [Re: jonas]
    #3435855 - 12/02/04 02:55 AM (12 years, 10 days ago)

Hey! i have a question about this. my jars were just inoculated last night. i put the foil back over the lids and put them in my TiT incubator. my question: do i need to remove the foil for colonization? I don't have Tyvek, just coffee filters--i figured they were better than nothing. so what so you, oh shroom masters?


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Offlinejonas
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Re: incubation question [Re: laughingbuddha]
    #3435874 - 12/02/04 02:59 AM (12 years, 10 days ago)

If you've got a coffee filter on I'd reccommend leaving the foil on. The coffee filters aern't made with any guidelines or specs, companies can make em however pourous or whatever they want, so theres a REAL good chance they won't keep out all contams. And nah, the foil never really has to come off during incubation.

And no, I'm not swedish. The name Jonas isn't my real name. It's kinda a nickname, me and my friend were just being dumb and high and we were making some absurd joke up or something and we came up with the character of Jonas, and we refer to each other as Jonas now and again. Its an inside joke I guess. Also the name of my first pipe :smile:


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OfflineMushroomFriend
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Re: incubation question [Re: laughingbuddha]
    #3435884 - 12/02/04 03:03 AM (12 years, 10 days ago)

LOL "shroom master" me hardly that.
But me reads carefull and listens when the real shroom masters like agar and magash are speaking! :laugh:

Coffee filter is not good filter, it gets wet and can cause contams. I suggest you (if u live in USA) got to postoffice and get some free tyvek envelopes. Cuta piece out of it, wipe with alcohol or H2O2, take coffee filter away, quickly replace it with tyvek. Best doing it in flowhood glovebox or oven.

If you have your alufoil too long and too tight the gas-exchange isnt possible. Then you could just as well glue the holes but the myc needs the holes.
For CO2 to escape mainly, so ive heard.

Ok further I have no backup on it but with only tyvek you will be fine!


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3435890 - 12/02/04 03:04 AM (12 years, 10 days ago)

K :P Jonas is a name often encountered in Sweden, thats why is asked...


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Offlinejonas
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Re: incubation question [Re: MushroomFriend]
    #3435898 - 12/02/04 03:07 AM (12 years, 10 days ago)

Haha, I figured. And the tyvek thing is a good idea, one suggestion tho is maybe take some tape, and tape off half or more of the tyvek to prevent too much moisture loss. I got tyvek envelopes from the post office for free like MF suggested and they're great.


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3435984 - 12/02/04 03:54 AM (12 years, 10 days ago)

I think tyvek is waterproof so wont need to tape it?

He could also tape his hole (lol) with bandaid.


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Offlinejonas
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Re: incubation question [Re: MushroomFriend]
    #3435994 - 12/02/04 04:04 AM (12 years, 10 days ago)

waterproof ... water vapor is another story...


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OfflineMushroomFriend
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Re: incubation question [Re: jonas]
    #3436002 - 12/02/04 04:13 AM (12 years, 10 days ago)

ok might be I thought water cannot pass the tyvek but air can


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Offlinejonas
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Re: incubation question [Re: MushroomFriend]
    #3436010 - 12/02/04 04:19 AM (12 years, 10 days ago)

well I had made a post a few days ago about my popcorn drying out a bit and I was told i left too much area exposed for air to pass through, basically the jars have only tyvek for lids. And I was told by several people on these boards that water vapor can pass through tyvek. I'm inclinded to believe what is said on these forums, there are some REALLY knowledgable people here.


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Offlinelesstutrey
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Re: incubation question [Re: jonas]
    #3436018 - 12/02/04 04:28 AM (12 years, 10 days ago)

tyvek is used for lining houses because it lets mositure out and in but not air and airborn contams. If you use tyvek.. use the inner lid.. put the tyvek over it, put sterile banaids over the the injection sites after inoculation.. make the injection holes bigger then usual so they are easy to find.. and there you go. water loss will be minimal.. gas exchange good and good filtration of contams. and yes.. coffee filters are bad, they are organic, and organic is bad.


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Offlinejonas
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Re: incubation question [Re: lesstutrey]
    #3436036 - 12/02/04 04:47 AM (12 years, 10 days ago)

Yeah, and if you make the mistake of throwing out the inner lids (as I did, haha) you can always just take up a portion of the tyvek.


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OfflineZeroArmy27
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Re: incubation question [Re: jonas]
    #3436606 - 12/02/04 11:35 AM (12 years, 10 days ago)

lesstutrey, very good. +5 for you, i was going to mention that.

i love tyvek because you can write on it. i put dots where my innoc holes are before i PC.

and yes jonas, if you don't have the inner lid, tyvek won't be enough because water vapor is small enough to pass through the tyvek. water in its' liquid state, however, will have a hard time.

the little band-aids they use when you get shots cover tyvek innoc holes perfectly. :smile:


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Offlinespliffmasta
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Re: incubation question [Re: ZeroArmy27]
    #3436628 - 12/02/04 11:44 AM (12 years, 10 days ago)

Put iodine in your water that your jars are sitting in.


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Amazon Shop for: ½ Pint Jars, Agar

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