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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Karo jars contam'd b4 inoc ,?'s
#3431415 - 12/01/04 03:20 AM (19 years, 3 months ago) |
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AFOAF says...
ok here it is , 8 jelly sized jars (smaller then 1/2 pints) were filled with 75ml distilled water and 1 teaspoon karo light , after being cleaned very well with alcohol , then tyvek lid with 1 poly-filled hole and 2 layers of foil , pc'd @ 15 psi for 20 mins and let cool for a day.
later 1 of the 7 had this very light brown sediment/growth/cloud collections on the bottom , removed foil to see that lid was drenched in this standing water, the rest looked clear with small fibers thinking to be from the paper towel used to clean them with no sediment.
so 7 were inoc'd with 4 different strains 1cc to each and covered with 2 coffee filters and rubber band, some actually pushed liquid up though hole upon puncturing (wiped off with alcohol) , is this ok (contams?)?
also since jars were so short syringe needle got submerged in solution , was then wiped with alcohol and flamed , is this ok (contams?)?
was wondering if the poly-filled hole (since moist) would wick contams into/around/through tyvek or puncture hole ?
first time doing karo and dont want to fuck up any help is appreciated thanks ~_^
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derx
who run it
Registered: 05/29/03
Posts: 2,459
Loc: dx/dt
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Re: Karo jars contam'd b4 inoc ,?'s [Re: Dr_BlueThumb]
#3431438 - 12/01/04 03:33 AM (19 years, 3 months ago) |
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It's very hard to interpret your thread precisely, you should consider editing it grammatically.
-------------------- better living through chemistry OVERGROW the government!! it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.
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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Re: Karo jars contam'd b4 inoc ,?'s [Re: derx]
#3431479 - 12/01/04 03:52 AM (19 years, 3 months ago) |
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dont understand , thought it read quite good , what can be done to help you help?
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lesstutrey
All Weather Associate
Registered: 10/24/04
Posts: 495
Loc: Chicagoland
Last seen: 9 years, 5 months
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Re: Karo jars contam'd b4 inoc ,?'s [Re: derx]
#3431484 - 12/01/04 03:53 AM (19 years, 3 months ago) |
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a few things no need for polyfill with tyvek..
were there chunks in the cloud? if so your karo is carmelized and probably no good... if its just lightly yellowish its good... you should see a distorted cloud upon shaking of the jar.. thats the karo built up on the bottom...
the needle in the solution is no big deal.
coffee filters invite contams and are useless with tyvek
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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Re: Karo jars contam'd b4 inoc ,?'s [Re: lesstutrey]
#3431493 - 12/01/04 03:58 AM (19 years, 3 months ago) |
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thanks for the relpy , u think AFOAF will be ok , dont understand how a coffee filter would invite contams as to help keep them out , please enlighten.
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lesstutrey
All Weather Associate
Registered: 10/24/04
Posts: 495
Loc: Chicagoland
Last seen: 9 years, 5 months
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Re: Karo jars contam'd b4 inoc ,?'s [Re: Dr_BlueThumb]
#3431498 - 12/01/04 04:02 AM (19 years, 3 months ago) |
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coffee filters are made of natural fibers, contams eat natural fibers. coffee filters get wet and stay wet for a while, and the it dispurses a lot.. contams like wetness. tyvek is a far superior filter, and is all you should need. organic is bad, thats why you use polyfill not cottonballs. and like i said, the polyfill is useless with the tyvek. infact, most say tyvek is superior to filter discs, and understandably so, eseapcaily if you have seen a picture of it under microscope. so here's my advice from for your FOAF, next time he does anything with tyvek, use it alone, its all you should need except for aluminum foil to cover while pcing.
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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Re: Karo jars contam'd b4 inoc ,?'s [Re: lesstutrey]
#3431500 - 12/01/04 04:06 AM (19 years, 3 months ago) |
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so do you think afoaf should remove the coffee filters on top of the jars and just leave the lid exposed or something else?
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 11 days, 10 hours
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Re: Karo jars contam'd b4 inoc ,?'s [Re: Dr_BlueThumb]
#3431511 - 12/01/04 04:11 AM (19 years, 3 months ago) |
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cover hole with polyfill or tyvek only.
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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Re: Karo jars contam'd b4 inoc ,?'s [Re: MushroomFriend]
#3431522 - 12/01/04 04:14 AM (19 years, 3 months ago) |
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lid assmbly (for a mental image)
xxxxxxx < rubber band |~~~~~| < coffee filter ==[]=== < lid with punched hole filled with polyfill _______ < tyvek | | |_____| <---jar
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 11 days, 10 hours
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Re: Karo jars contam'd b4 inoc ,?'s [Re: Dr_BlueThumb]
#3431533 - 12/01/04 04:19 AM (19 years, 3 months ago) |
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ow thats easy to take coffee filter away, if there is a hole in the tyvek as well, you could let the polyfill stay there. So keep lid + tyvek (and maybe polyfill) remove coffee filter.
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Dr_BlueThumb
Stranger
Registered: 11/17/04
Posts: 17
Last seen: 19 years, 1 month
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Re: Karo jars contam'd b4 inoc ,?'s [Re: MushroomFriend]
#3431542 - 12/01/04 04:26 AM (19 years, 3 months ago) |
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ok thank you does anyone else agree with this?
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 7 months
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Re: Karo jars contam'd b4 inoc ,?'s [Re: Dr_BlueThumb]
#3431747 - 12/01/04 07:27 AM (19 years, 3 months ago) |
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hmm dr thumb you have to undertsand there are alot of people on here who don't undertsand american english short form typing we like to cut corners brief it up...i gave up on karo jars mine didn't seem to pan out..i don't feel like fucking with them again..maybe on my last bit of spore jiuce i can give it a whirl but for now im kool with just knocking up my wbs jars with spores.
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