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Offlinelargosnook
2 glassesicewaddawiddice
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Registered: 11/03/04
Posts: 314
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Kero Q
    #3427832 - 11/30/04 01:10 PM (19 years, 7 months ago)

I made some kero solution and sterilized in the microwave. the Seran wrap sealed it up nice and i put the lid on it. Its cooling now but when i just checked it, the sides have been sucked in like there is negative pressure in there. How do i release the pressure without it sucking any contams in? When i stick my needle in it, its gonna suck air in. Any advice?

Plus I can see a few small, clear chunks floating in the solution. Is this bad?

Edited by largosnook (11/30/04 02:03 PM)

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OfflineFakeRealism
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Posts: 54
Last seen: 19 years, 2 months
Re: Kero Q [Re: largosnook]
    #3427866 - 11/30/04 01:21 PM (19 years, 7 months ago)

Since you have a layer of seran wrap...you could tape a patch of tyvek on it and punture the seran wrap through the tyvek. It would be to your advantage to have some air exchange anyhow.

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Offlinelargosnook
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Re: Kero Q [Re: FakeRealism]
    #3427889 - 11/30/04 01:28 PM (19 years, 7 months ago)

i would have a hole in the tyvek as well, wouldnt non-sterile air still get in?


Ive turned a few people on to Fake Realism, congrats

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Offlinelargosnook
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Re: Kero Q [Re: largosnook]
    #3428041 - 11/30/04 02:03 PM (19 years, 7 months ago)

and the chunks?

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Offlinelemon_lw
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Registered: 10/17/04
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Re: Kero Q [Re: largosnook]
    #3428764 - 11/30/04 04:26 PM (19 years, 7 months ago)

i put 3 layers on my karo jars. one layer then lid then next layer then one more. i lift the very top layer and inoc through the first 2 layers. chunks could be carmalized karo but ive never done that so i dont know what it looks like.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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Offlinespliffmasta
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Re: Kero Q [Re: lemon_lw]
    #3429062 - 11/30/04 05:24 PM (19 years, 7 months ago)

Great idea lemon  :thumbup: :thumbup:

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InvisibleNOS4A2
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Registered: 07/22/04
Posts: 572
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Re: Kero Q [Re: spliffmasta]
    #3429118 - 11/30/04 05:39 PM (19 years, 7 months ago)

Did this guy even use a "jar"? can't suck the sides into a jar
Another suggestion Use a whole lid with a hole off center just big enough for da needle. Then cover hole with small round breathable bandaids everytime you stick a needle in!


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Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

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Posts: 9,399
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Re: Kero Q [Re: largosnook]
    #3429192 - 11/30/04 06:01 PM (19 years, 7 months ago)

could be carmaliezed karo,
could also be residue from some other use the jar may have had, if it wasn't cleaned properly
you should be okay either way
as long as you nuked long enough.
as far as your pressure problem,
make sure to put the lids on yr jars so the shiny side of the top lid is facing into the jar
vacuums are not your friends


--------------------
:beakedwhale:

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Offlinedjred
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Re: Kero Q [Re: muse_sick]
    #3429405 - 11/30/04 06:38 PM (19 years, 7 months ago)

man made some akro jars a week ago and they are not showing any real growth..i have like a msit of white in the bottom..kinda strings together.but from the pics i see, its nothing to count on. im sure when i had started i may have over heated the jars in the incubator..thinking that may have killed the spores? well if no contams are showing would it be possible to just throw the karo in some bulk poo straw

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Offline4hodmt
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Registered: 04/06/04
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Re: Kero Q [Re: djred]
    #3429425 - 11/30/04 06:44 PM (19 years, 7 months ago)

c hunks are caramalized. take ur handy dandy syuringe, and suck sum air up through the center of a flame into it, then innoc, and squirt the air in too. :-D.

cover witha band-aid


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


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"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler

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Offlinedjred
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Re: Kero Q [Re: 4hodmt]
    #3429439 - 11/30/04 06:46 PM (19 years, 7 months ago)

ok your telling me to shoot hot air into the karo jar? i have a marble in the jar..the white shit kinda looks like a bunch of sugar grains stuck together..i can see loads of spores also..kinda looks like dust in water..but its deff not contams.

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Invisiblemuse_sick
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Re: Kero Q [Re: djred]
    #3429582 - 11/30/04 07:32 PM (19 years, 7 months ago)

i would just leave it alone for a few days more
check your temps, if they are either to high or two low
(more than a few degrees from 86f)
then your germination can be fucked up
myc looks very similar to what you described as far as being stringy and white
so i would guess you are expericing delayed colonization
don't do anything drastic like try to use karo to spawn directly to bulk
that's only gonna result in contams


--------------------
:beakedwhale:

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Offlinedjred
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Re: Kero Q [Re: muse_sick]
    #3429610 - 11/30/04 07:41 PM (19 years, 7 months ago)

gotcha im going to give them another week or so..no real hurry i have plent yof spores still..um yeah they got up in th eupper 90's there fora minute so..but i do have my temps in check

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Invisiblemuse_sick
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Re: Kero Q [Re: djred]
    #3429725 - 11/30/04 08:11 PM (19 years, 7 months ago)

that's your problem
if you don't see increased colonization soon, i would prob start from scratch with fresh spores


--------------------
:beakedwhale:

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Offlinedjred
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Re: Kero Q [Re: muse_sick]
    #3429949 - 11/30/04 09:05 PM (19 years, 7 months ago)

thanxs i figure di cooked my spores..i finally got a tnt yay..went to big lots got to 4 gallon tubs..im using a heating pad on low setting on bottom and another one on low behind the tubs. plus they are being kept in dresser cabinet. so the heat kinda builds up.. can i stick my digital therm to the sides of the tnt to check the temp? pretty much what ever the wall is give a 1 to 2 deg diff ..compared to the water?

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Offlinedjred
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Re: Kero Q [Re: djred]
    #3429955 - 11/30/04 09:06 PM (19 years, 7 months ago)

when i get payed friday im going to buy a fish tank heater..the ones i seen at walmart had a decent price. going to check petsmart..

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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 10 years, 2 months
Re: Kero Q [Re: djred]
    #3429994 - 11/30/04 09:17 PM (19 years, 7 months ago)

def. shoot air in those shits, if its not too late... if not, whenever u inject spores, itll just suck sum air from outside (  :crazy:! ) contams would come in. inject air.


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler

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Offlinedjred
newbie

Registered: 08/27/04
Posts: 922
Last seen: 15 years, 11 months
Re: Kero Q [Re: 4hodmt]
    #3430023 - 11/30/04 09:22 PM (19 years, 7 months ago)

oh i see...well thie rnot contamed..made sure plenty of oust lysol was sprayed around me on me..i cooked my spores to hot im sure of it .i do have some growth.. but im not concerned with my karo jars fuck it..

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