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OnlineGreat Scott
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Sterilizing Syringes
    #3425701 - 11/30/04 12:41 AM (13 years, 5 months ago)

Hi, i need to make a syringe out of a spore print i have lying around.  The print is in a jar, so i figured i could squirt 10 cc's into the jar, shake, and then suck the water back up once the spores are mixed into the water.
I drew 10 cc's of water into my syringe and placed the syringe in a pot of boiling water for 20 minutes (to sterilize the water).  But the problem is...most of the water in the syringe  evaporated  :smirk:  There were only 2 cc's left.

So how can i prepare some sterlized syringes (containing sterilized water) ???
:tongue2:


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OfflineRRhoads
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Re: Sterilizing Syringes [Re: Great Scott]
    #3425729 - 11/30/04 12:49 AM (13 years, 5 months ago)

boil some water, then suck it up :grin:


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InvisibleLifenergy
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Registered: 08/05/04
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Re: Sterilizing Syringes [Re: RRhoads]
    #3425741 - 11/30/04 12:52 AM (13 years, 5 months ago)

If your print is okay, and you have more than one syringe, you should make a few. Stretch that print out a little. And let the water cool to room temp in the syringe before you mix it with the spores or you'll kill them.


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Everything that we see is a shadow cast by that which we do not see.


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InvisiblePsychoslut
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Re: Sterilizing Syringes [Re: RRhoads]
    #3425743 - 11/30/04 12:52 AM (13 years, 5 months ago)

yeah, boil water, then suck it up and push it out repeat like 4 times, then suck up a final sucking (lotta suckage lol) and let that cool off completely, i run mine under cold tap water. Then flame sterilize the needle and do what you was gonna do.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineRRhoads
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3425781 - 11/30/04 01:00 AM (13 years, 5 months ago)

you like sucking, yes?


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OnlineGreat Scott
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3425793 - 11/30/04 01:01 AM (13 years, 5 months ago)

sweet. Thanks for the advice.
I have several diff. things i can try now.


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InvisiblePsychoslut
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Re: Sterilizing Syringes [Re: RRhoads]
    #3425811 - 11/30/04 01:05 AM (13 years, 5 months ago)

Quote:

RRhoads said:
you like sucking, yes?





:yesnod:


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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InvisibleLifenergy
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3425820 - 11/30/04 01:07 AM (13 years, 5 months ago)

The most important things are sterilize the water, sterilize the syringe, use a sterile print, and sterilize the surfaces you will be using. And oh yeah, sterilize yourself.


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Everything that we see is a shadow cast by that which we do not see.


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OfflinepsilocybinjunkieM
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Re: Sterilizing Syringes [Re: RRhoads]
    #3425940 - 11/30/04 01:40 AM (13 years, 5 months ago)

Quote:

RRhoads said:
boil some water, then suck it up :grin:



This man speaks the truth :spliff:
Thats what you have to do...


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InvisibleMagashM
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Re: Sterilizing Syringes [Re: Great Scott]
    #3426151 - 11/30/04 02:25 AM (13 years, 5 months ago)

I just fill em with bottled water. Put em in a jar (so the needles face up or water will leave the syringe when the water in it boils) and PC them.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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InvisiblePsychoslut
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Re: Sterilizing Syringes [Re: Magash]
    #3426175 - 11/30/04 02:30 AM (13 years, 5 months ago)

i put way too much spores in my syringe today, and i inoculated pf jars, and didn't have enough solution. so i diluted 3 cc of my super dark spore syringe by putting a gallon of sterile water, an alcohol swab, and my syringe and Lysol in my glove box, i swabbed a section of the water jug, and the poked the needle in and sucked in water. do you think i contamned it by doing that?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflinepsilocybinjunkieM
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3426202 - 11/30/04 02:37 AM (13 years, 5 months ago)

it might...
but you should be fine :wink:


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InvisibleMagashM
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3426222 - 11/30/04 02:43 AM (13 years, 5 months ago)

Naw, you should be safe but as far as using to many spores.

Ok here come the flames but I've ran many many tests on this and I say the more the marrier as far as spores go and if you make your own it's easy as hell anyway. Here are the syringes I like to use



--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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InvisiblePsychoslut
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Re: Sterilizing Syringes [Re: Magash]
    #3426262 - 11/30/04 02:53 AM (13 years, 5 months ago)

i know i couldnt of had too much spores, really i just didnt want to make another syringe tonight, so i was like screw it.  :grin:


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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InvisibleMagashM
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Re: Sterilizing Syringes [Re: Psychoslut]
    #3426277 - 11/30/04 02:56 AM (13 years, 5 months ago)

:wink: I hear ya.  :thumbup:


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflinepsilocybinjunkieM
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Re: Sterilizing Syringes [Re: Magash]
    #3426401 - 11/30/04 03:23 AM (13 years, 5 months ago)

i agree with you on the more the marrier. dark syringes get the job done and are more apealing to the eye~


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Invisiblekandymews
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Re: Sterilizing Syringes [Re: Magash]
    #9700695 - 01/29/09 05:52 PM (9 years, 3 months ago)

How long do you PC them, Im assuming 45 mins @ 15psi, or wouldnt they need as long seeing as people rinse them repeatedly with boiling water, which would not be as sterile as the PC [in theory]?


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OfflineMagic_Hobo
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Re: Sterilizing Syringes [Re: kandymews]
    #9700845 - 01/29/09 06:18 PM (9 years, 3 months ago)

I just PC them with my grain jars for 90 min.

In other news, this thread is over 4 years old.


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