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Offlinewideweb
fungisist
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Registered: 11/11/04
Posts: 141
Last seen: 5 years, 4 months
Culture Syringe
    #3422625 - 11/29/04 12:31 PM (13 years, 5 months ago)

How do I make culture syringe (as opposed to spore syringe)?
What's the "shelf life" of such syringe?
Do I have to refrigerate it?
Thanks


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InvisibleKoala Koolio
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Registered: 01/07/04
Posts: 7,752
Re: Culture Syringe [Re: wideweb]
    #3422720 - 11/29/04 01:10 PM (13 years, 5 months ago)

Karo tek is one method, its a liquid mycellium culture.

Not sure on shelf life, but refridgeration will definately extend it.


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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: Culture Syringe [Re: Koala Koolio]
    #3423018 - 11/29/04 02:47 PM (13 years, 5 months ago)

I inject about 10 cc of sugar water(1cc molasses to 300 ml water)
inside a testube slant that I have grown over with myc then attach it to a little motor that does about 30 RPM.
I leave a little air bubble inside to stir things up and let it run for 3 days.
The mycelium grow thru the water and some of the PDA dissolves.
Pop it suck it up and inject your whatever.
It covers the seed with liquid food and the colonization is creepy fast.

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Offlineragadinks
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Registered: 10/20/03
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Re: Culture Syringe [Re: Peterthinks]
    #3423317 - 11/29/04 04:13 PM (13 years, 5 months ago)

I was able to "revive" a culture syringe with reishi mycelium after 6 month in the fridge ...


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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