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Today I looked to see if anything had happened as far as growth in my jars that I have recently inoculated...all 6 of my eq jar have gone bad and I dontknow why.....must have been a bad syringe or something. It had to have been because I don't know what else may have cause something like that.....it is like a green stinky wet spot in the jar...you cant really tell yet if it is green but it smells bad and it looks like nasty wet spots...could the cause be because of too much innoculant to initiate the culture? I used a whole fucking syringe on six jars...then inoculated 20 others with a single syringe and ALL those jars are doing just fine...Let me know what you think.
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Hard to say; Keeping at least one jar aside, uninoculated, as a negative control is very helpful in pinpointing the source of problems. If it goes bad, you know something is amiss with the substrate prep/sterilization/cooling steps. Because you used a second syringe successfully, this basically accomplishes the same thing. Something in that first inoculation went awry; Whether you just added too much liquid to each jar [which can encourage problems] or whether the syringe was contaminated, is hard to say for for sure.
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