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OfflineMushroomFriend
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Mycelium in KAro
    #3416880 - 11/27/04 07:48 PM (12 years, 8 days ago)

Hello all

Thursday night I put a small piece of mycelium from a slow PF cake in a jar with 100ML karowater.

WHen should I see growth if any?

Must have been posted and answerd be4 but searching on karo and mycelium gives millions of results... :p

Thanks in advance! :thumbup:


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Offlinelemon_lw
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3416895 - 11/27/04 07:51 PM (12 years, 8 days ago)

i cant tell you mycelium into karo but spores to karo right around a week.


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Invisibleandjor
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Re: Mycelium in KAro [Re: lemon_lw]
    #3416932 - 11/27/04 07:59 PM (12 years, 8 days ago)

If you put a piece of mycelium in the karo, you should see growth from that myc fairly quickly. Probably within a couple days.


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Offlinehawksapprentice
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Re: Mycelium in KAro [Re: andjor]
    #3416971 - 11/27/04 08:08 PM (12 years, 8 days ago)

Depends on what temps you are incubating the karo mix at. If its only been two day keep waiting man. Ive found mycellium can grow a little slower in this solution.


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OfflineMushroomFriend
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Re: Mycelium in KAro [Re: hawksapprentice]
    #3416978 - 11/27/04 08:10 PM (12 years, 8 days ago)

Yes ill wait, havent got any place left in my TIT right now to inoc new jars abyway but am just being curious.

Im incubating at 82-86F.

Thanks guys for the quick reactions.....


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OfflineMushroomFriend
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3416984 - 11/27/04 08:13 PM (12 years, 8 days ago)

Thats also good to know cause i also have a tiny karo jar (100ML) with 2CC of GT sporewater in it... ! :laugh:

By the way I think I used dark corn syrup cause i used 1 tsp on 100 Ml and it is very yellow, not that clear as in the teks with light syrup.
Any harm exept worse view?


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Offlinelesstutrey
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3417182 - 11/27/04 09:08 PM (12 years, 8 days ago)

it usualy takes me a week or so, maybe 4-10 days for myc to show up in my karo jars, even with liquid inoc.. took maybe 15 days for something that was highly viable and useable. and no harm except the view


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Invisibleandjor
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Re: Mycelium in KAro [Re: lesstutrey]
    #3417273 - 11/27/04 09:40 PM (12 years, 8 days ago)

If it is dark, you might have carmelized the sugars.


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Offlinediscman1
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Re: Mycelium in KAro [Re: andjor]
    #3417482 - 11/27/04 10:43 PM (12 years, 7 days ago)

Uh oh.

Look on your bottle of corn syrup. Does it say Light Corn Syrup?

If so, you've got the right stuff, and you somehow caramelized the sugars in your solution.

Caramelized sugars are undesirable and both retard growth and promote genetic mutations.


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OfflineMushroomFriend
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Re: Mycelium in KAro [Re: lesstutrey]
    #3418553 - 11/28/04 06:34 AM (12 years, 7 days ago)

There a lot of small fluffy thingies in the jar, but can be that they loosend from the verm/myc piece I put in..

Ok good to hear no harm, next time ill try to buy some light syrup.
Me gonna experiment with different other syrups too which are more easily available.
In teh eko store here they have rice syrup and wheat syrup and so on...


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OfflineZeroArmy27
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3418740 - 11/28/04 09:03 AM (12 years, 7 days ago)

the only problem with the dark karo (which i use in my pecan pie, mmmm) is that it's harder to tell if you're contamed or not.

light syrup leaves the solution clear, so you can see the fluffy white things floating around, and any discoloration is very apparent.


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Offlinedjred
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Re: Mycelium in KAro [Re: ZeroArmy27]
    #3418753 - 11/28/04 09:21 AM (12 years, 7 days ago)

agrees i made to jars of karo im on day 4 at day 2 i could see mycelium growing,it was almost a cloudy patch on the bottom..like white floating particals. i tried a batch of half of 8 oz whole wheat cakes just to mess around sinc ei have so much spore solution i still have like 30 cc of spores left..with 2 karo jars going..so i keep my syringes in the fridge


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OfflineMushroomFriend
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Re: Mycelium in KAro [Re: djred]
    #3418758 - 11/28/04 09:24 AM (12 years, 7 days ago)

did u inoculate with spores? fast germination then.


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Offlinedjred
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3418785 - 11/28/04 09:47 AM (12 years, 7 days ago)

yes i did spores, well when i made the jars they were crystal clear. couple of days later i see spores galore floating around in the karo water..then you can see the myc on the bottom starting to form.


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Re: Mycelium in KAro [Re: djred]
    #3418788 - 11/28/04 09:48 AM (12 years, 7 days ago)

MushroomFriend.. If your water is caramelized, you would be advised to start over. It is undesirable.


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OfflineMushroomFriend
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Re: Mycelium in KAro [Re: discman1]
    #3418825 - 11/28/04 10:24 AM (12 years, 7 days ago)

Yes IF, but I dont think it is. how does one notice that?


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Offlinedjred
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3418853 - 11/28/04 10:45 AM (12 years, 7 days ago)

kinda slow on here today...


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Offlinediscman1
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Re: Mycelium in KAro [Re: MushroomFriend]
    #3420653 - 11/28/04 09:56 PM (12 years, 7 days ago)

Quote:

MushroomFriend said:
Yes IF, but I dont think it is. how does one notice that?


Like I said, if you used Light Corn Syrup and your water is anything but crystal clear(brown, yellow), you have caramelized sugars.



Cambodian, 2 weeks after inoculation(spores).


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Offlinejonas
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Re: Mycelium in KAro [Re: discman1]
    #3420716 - 11/28/04 10:08 PM (12 years, 7 days ago)

You can use a microwave to sterilize karo jars, right?


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Offlinelemon_lw
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Re: Mycelium in KAro [Re: jonas]
    #3420729 - 11/28/04 10:10 PM (12 years, 7 days ago)

or you can use boiling water


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