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Offlinegreen_guy
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Registered: 06/11/01
Posts: 18
Last seen: 15 years, 11 months
Inoculation without a syringe
    #341489 - 06/15/01 03:29 PM (15 years, 11 months ago)

So I was stupid and ordered a spore print without a syringe... I've combed over my whole frickin' town and can't seem to locate a syringe anywhere, and don't have the time to wait another week or two for one to come in the mail. Let's say, if I bleached a small empty airtight closet very well, was extraordinarilly sterile about the whole process (hat, mask, gloves, goggles, and long sleeves) would I stand any chance of being able to successfully avoid contamination? I'm going to try twelve jars, with the expectation of a few maybe failing. Has anyone ever done it this way? I'd do flip trips over any good advice on the matter. Thanks.



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Offlinelounge lizard
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Registered: 01/07/01
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Re: Inoculation without a syringe [Re: green_guy]
    #341516 - 06/15/01 04:01 PM (15 years, 11 months ago)

If you grew on rye, you could use the spore print to innoculate.  But I don't think that innoculating PF style jars with a spore print would give you good results.  Unless you have air filters and a clean lab, I would just wait until you can get a syringe.  Search through the teks on the grow/find page and you might find something that could help.

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Offlinepyromaniac
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Re: Inoculation without a syringe [Re: green_guy]
    #341523 - 06/15/01 04:10 PM (15 years, 11 months ago)

Hey green guy I did the same thing, I rdered a print from spore wroks but no syringe. I have no clue why cuz I didnt have a syringe to use. I however went back and bought ten syringes from them because I didn't ever want to be without one. I now have like 5 unopened ones that may not be opened for a long time but at least I've got em. Why don't yu have time for one to come in the mail? I was able to wait even though the entire time I felt like just trying to innoculate without a syringe.

Chances are, if you try to innoculate PF style jhars it wont work. I dunno why, it just seems right to me. Don't the spores need to rehydrate or something before they can germinate?

Hell man, if you are that desperate give it a go and see how it works out but I would reccomend waiting for one to come in the mail.

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OfflineNagual
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Re: Inoculation without a syringe [Re: green_guy]
    #341612 - 06/15/01 06:08 PM (15 years, 11 months ago)

There's an oven tek that says if you pull out your lowest oven rack with the oven on the lowest heat setting the contaminates are kinda pushed away by the warm rising air. But i guess the rice flour/vermiculite cake -optimally- needs to be injected and not just sprinkled with spores. I don't understand.. what are the goggles for? Do you have dirty eyes?

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Offlinegreen_guy
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Re: Inoculation without a syringe [Re: Nagual]
    #341623 - 06/15/01 06:15 PM (15 years, 11 months ago)

The goggles are orange tinted and make me feel like an action hero... that's all. Also, I gave up on the idea of risky maneuvers and realized that it will be worth waiting a couple days to get a whole whoppin' ten syringes from sporeworks. Shoot. I was reading Shroom Wizard's guide where the syringe step is skipped, but now come to think of it, the spores probably do need to be rehydrated before getting tossed into their little glass houses. I suppose you can't go wrong with sticking to the Mushroom Bible (PF TEK) every step of the way. In the meantime, I think I need to go to support groups for impatient people.



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Offlineegghead
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Registered: 04/25/01
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Re: Inoculation without a syringe [Re: green_guy]
    #341660 - 06/15/01 06:55 PM (15 years, 11 months ago)

green guy.. you might try sterilizing some toothpics in the oven and innocing w/ them. dip a sterile toothpick into the some sterile water, then use the moist end to collect some spores off the print. Then jam the whole toothpick into one of your innoc holes. You could probably just leave them in the jars.

Sound like a winner?



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OfflineMNmyc
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Re: Inoculation without a syringe [Re: egghead]
    #341702 - 06/15/01 07:55 PM (15 years, 11 months ago)

Don't EVER collect spores by introducing humidity to the print medium, unless you want to be agar king (capillary actoin). I have enough issues without that.

But I wouldn't be opposed to a sterile dry pick that happened to get a drop or two of liquid on the OPPOSITE end (enough to hydrate by capillary action), AFTER collection, and THEN introduced to a spawn medium.

Success could be greatly improved, in (my) theory, by only using the 'best' toothpicks after a short period of incubation. And the picks make nice 'running lanes' that make aggressiive growth easy to see. Strains next to each other would look like a DNA analysis.

Maybe a bunch o' shit, but it sounds good to me...

I think Prellgot made me think about toothpicks.

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Offlinegreen_guy
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Registered: 06/11/01
Posts: 18
Last seen: 15 years, 11 months
Re: Inoculation without a syringe [Re: egghead]
    #341738 - 06/15/01 08:57 PM (15 years, 11 months ago)

Blazing good idea. I might just try it for the sake of discovery.



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InvisibleBillyblastoff
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Re: Inoculation without a syringe [Re: green_guy]
    #341754 - 06/15/01 09:30 PM (15 years, 11 months ago)

"Green guy" if you want a syringe or two PM me an addy....
Billy

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OfflineFungusFlipper
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Registered: 05/26/01
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Last seen: 15 years, 8 months
Re: Inoculation without a syringe [Re: Billyblastoff]
    #341756 - 06/15/01 09:32 PM (15 years, 11 months ago)

also go to home depot or lowes and ask for turkey basting syringes. they are sweet.

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Offlinecobz
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Registered: 12/30/00
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Loc: Sydney
Last seen: 7 years, 4 months
Re: Inoculation without a syringe [Re: FungusFlipper]
    #341955 - 06/16/01 02:56 AM (15 years, 11 months ago)

green_guy, i tried just scraping sproes into my jars awhile ago and it failed, so i went to my local chemist and got a syringe then it worked hehe




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OfflineEvilG
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Registered: 07/19/06
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Re: Inoculation without a syringe [Re: cobz]
    #5878789 - 07/19/06 09:04 PM (10 years, 10 months ago)

In a pinch couldn't you boil some straws in the microwave and pick up the re hydrated spores by putting one end of the straw into the water and a finger over the other end to hold the liquid in the straw, then releasing it slightly above the substrate to inoculate.


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Invisiblefastfred
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Re: Inoculation without a syringe [Re: EvilG]
    #5878847 - 07/19/06 09:20 PM (10 years, 10 months ago)

Yeah, mabe if you had come up with this idea 5 YEARS AGO...


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