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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Pressure Cooker, Wild Bird Seed

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InvisibleTHEDANGLER
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Cyan germination
    #3411502 - 11/26/04 10:43 AM (12 years, 7 months ago)

I did 5 jars 2 oven bags, a couple petries and some small jars, all jars are birdseed and the agar is the easy dog food agar tek. Oh i also did a Karo jar. its been about 1 week none have germinated, i don't think its the conditions of the environment because my cambo jars are working and i was more sterile with the cyan's, the jars are all in the same place in a box on a heating pad. Could the incubation temp be to much i remember reading cyan's are cold climate fun-guys.

Whats the diagnosis


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InvisibleLifenergy
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Re: Cyan germination [Re: THEDANGLER]
    #3411507 - 11/26/04 10:45 AM (12 years, 7 months ago)

What type of cyans? Pan or Psil? Check the temp on your box, the bottoms of jars can get cooked from using a heating pad and the bottom becoming too hot.


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InvisibleTHEDANGLER
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Re: Cyan germination [Re: Lifenergy]
    #3411519 - 11/26/04 10:50 AM (12 years, 7 months ago)

they are pan. cyans australian, the pad is on low so their not geting cooked, the cubes are doing fine.

Im thinkin bad syringe.


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InvisiblePrisoner#1
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Re: Cyan germination [Re: THEDANGLER]
    #3411665 - 11/26/04 11:44 AM (12 years, 7 months ago)

here's a link from hamurabi, he just did a grow log on them
do a search in grow logs for alounacara as well, it may help you
to see if there is something your doing wrong

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3323991/an/0/page/0


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Invisibleandjor
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Re: Cyan germination [Re: Prisoner#1]
    #3411807 - 11/26/04 12:19 PM (12 years, 7 months ago)

Might be a bad syringe. Pans have a very fast growing mycelium, so unless this is a really old syringe, you should have probably seen something by now.


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InvisibleTHEDANGLER
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Re: Cyan germination [Re: Prisoner#1]
    #3411812 - 11/26/04 12:21 PM (12 years, 7 months ago)

Ive read lots of those grow logs, most say that the vegetative temp is about the same as cubes, i can't figure out the problem. The only thing that is feasible is that the syringe was bad or had a lack of spores, or they were nonviable. I'm going to wait a week and if not germination occurs than i will contact the vendor.


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Edited by THEDANGLER (11/26/04 12:41 PM)


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InvisiblePrisoner#1
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Re: Cyan germination [Re: THEDANGLER]
    #3411817 - 11/26/04 12:24 PM (12 years, 7 months ago)

be a bit more patient, some times it takes a little while, I never write it of until about the 3rd week.


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Invisibleandjor
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Re: Cyan germination [Re: Prisoner#1]
    #3411968 - 11/26/04 01:16 PM (12 years, 7 months ago)

Does anyone know what strain of pan cyans it is that Ralphster sells?


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InvisibleTHEDANGLER
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Re: Cyan germination [Re: andjor]
    #3412020 - 11/26/04 01:33 PM (12 years, 7 months ago)

I think it was Australian, he was the person i ordered them from because i know he is the cyan guru, i know it is not his fault, could just be a bad batch or a slow strain i got stuck with.


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Invisibleandjor
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Re: Cyan germination [Re: THEDANGLER]
    #3412810 - 11/26/04 05:28 PM (12 years, 7 months ago)

ok, thanks. Foaf got a pan syringe from him too. It showed amazing growth in the wbs jar. He spawned to poo/straw after a week, but it got covered in cobweb and smeleed really funny.
Now he isn't sure whether it was contaminated from the start or what because he hasn't had trouble with parturizing poo/straw before.
He didn't usee the whole syringe and is going to try again in a bit, so he will see what happens then.


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Offlinescatmanrav
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Re: Cyan germination [Re: andjor]
    #3413120 - 11/26/04 07:03 PM (12 years, 7 months ago)

I've heard pans like about 10 degrees cooler then cubies in both stages..try pulling them out of the incubator. I'm sure a pan expert could correct me if I'm wrong..

Pan cyans are also a very weak mycelium species..you may have to use an isolate or wait longer for nore moticable growth but look closly it will be very wispy. Most people start with isolates though.

If you got a fully colonized jar and then spawned to something and it contamed then it was not there from the start. Mold spores will not sit and not germinate..if they existed from the start they'd have destoryed the wbs jars.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleTHEDANGLER
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Re: Cyan germination [Re: scatmanrav]
    #3416309 - 11/27/04 05:34 PM (12 years, 7 months ago)

I did pull them from the box today, 5 bird seed jars, 2 Karo jars, 12 agar jars, none with any growth, in every step of my process i was very clean, used a p-cooker and a glove box. THIS IS SOME BULL SHIT> I emailed the vendor here is what he wrote back.

Bro
we let you keep the cambo. And i don't think that WBS, Karo, and dfa is a good substrate. Please understand.

What the fuck PAN CYANS wont grow on birdseed Karo syrup or agar.

Could some one help me out and trade me a Pan cyan print.


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InvisibleTHEDANGLER
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Re: Cyan germination [Re: THEDANGLER]
    #3416649 - 11/27/04 06:57 PM (12 years, 7 months ago)

NO one out their can help me?


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InvisibleSoopaX
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Re: Cyan germination [Re: THEDANGLER]
    #3417418 - 11/27/04 10:20 PM (12 years, 7 months ago)

Ralph sells a Vietnamese pan cyan strain. When did you order the syringes? Pan Cyans usually show growth a day or two before cubensis that were done at the same time. Wild bird seed works great. PM me if you want some pan cyan prints, although it will probably be a few more weeks before they are available.


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Offlinescatmanrav
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Re: Cyan germination [Re: THEDANGLER]
    #3419789 - 11/28/04 06:14 PM (12 years, 7 months ago)

Quote:

THEDANGLER said:
I did pull them from the box today, 5 bird seed jars, 2 Karo jars, 12 agar jars, none with any growth, in every step of my process i was very clean, used a p-cooker and a glove box. THIS IS SOME BULL SHIT> I emailed the vendor here is what he wrote back.

Bro
we let you keep the cambo. And i don't think that WBS, Karo, and dfa is a good substrate. Please understand.

What the fuck PAN CYANS wont grow on birdseed Karo syrup or agar.

Could some one help me out and trade me a Pan cyan print.




PDA would be better.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleEonTan
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Re: Cyan germination [Re: THEDANGLER]
    #3427184 - 11/30/04 11:34 AM (12 years, 7 months ago)

That doesn't sound like ralph. :confused:

Panaeolus/Copelandia cyanescens grows mycelium on any of those media. It will even fruit, poorly, on seed.

Temperatures between 70-85 F will work. The colder it is the longer it takes.  Fruiting is better at the 80F mark then at 70F for most strains in circulaiton.

Fresh spores germinate quickly, typically within 3 days if they are already hydrated. Older spores can take up to 3 weeks or more to germinate, even when hydrated.

Cotamination could prevent growth, but it would typically be visible it self.

Sounds like a bad syringe to me.

Any Vendor should replace a syringe that does not contain healthy spores of the species/Strain purchased for microscopy. Viewing collapsed spores does not contribute to proper Identification and refrencing against other Species/Strains.

Vendors should refuse to replace spores to someone who openly admits to them in writing that their intent was to grow the spores. Spores are for Microscopy!

A simple. When I put the spores under my scope there was very few per mount, and those that were there were collapsed and malformed. Can you please replace this syringe with one that contains enough spores that I can see more then 2 per drop of solution, and they are all not malformed. :tongue:


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OfflineHamurabi
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Re: Cyan germination [Re: EonTan]
    #3427473 - 11/30/04 01:25 PM (12 years, 7 months ago)

it sounds like a bad syringe!!
pan cyans want definitely the same temperatures as cubes and at the fruiting stage they like even higher temps!!!


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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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