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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Rye Grain

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Offlineqwon
destroying angel
Registered: 08/21/04
Posts: 324
Last seen: 4 years, 2 months
make shift sterile environment for GTG
    #3396130 - 11/23/04 12:31 AM (12 years, 6 months ago)

anyone ever made one with a clear rubbermaid container, two holes cut for rubber gloves which are then sealed from the outside (so you could work inside the tub without opening the tub (once the elements are in there) - - any thoughts or suggestions?


--------------------
'I'm the commander see, I don't need to explain, I do not need to
explain why I say things. That's the interesting thing about being the president.
Maybe somebody needs to explain to me why they say something,
but I don't feel like I owe anybody an explanation.'
- George W. Bush


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: make shift sterile environment for GTG [Re: qwon]
    #3396216 - 11/23/04 12:56 AM (12 years, 6 months ago)

it's been done plenty of times...
do a search for glove box, choose 1 year, in subject only and main posts
and that will yeild you the best results

here's one
My First Glovebox


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Offlinemushroommark
Earning mybluethumb

Registered: 10/01/04
Posts: 359
Last seen: 10 years, 9 months
Re: make shift sterile environment for GTG [Re: qwon]
    #3396222 - 11/23/04 12:58 AM (12 years, 6 months ago)

I just did some g2g about 10 min ago with a rubbermaid with holes cut for arms and and a simply piece of plastic hanging over the holes. Set it in my bathroom cleaned thoroughly, ran hepa for an hour, lysol and oust like crazy. I'll let you know how it goes...

However, the colonized jar that I did the grain to grain with was initially colonized using a colonized PF cake that I cut a chunk off and threw into the rye jar. It colonized in less than a week with no problems.

Oh yeah, I also wore latex gloves and a mask and antibacterial soap up my arms real good. So it seems that it can be done...


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Offlinedmc_
211
 Arcade Champion: Madness

Registered: 12/24/03
Posts: 559
Loc: Portland, OR
Last seen: 11 years, 8 months
Re: make shift sterile environment for GTG [Re: Prisoner#1]
    #3396226 - 11/23/04 01:00 AM (12 years, 6 months ago)

I wash my hands with antibacterial, lay a sterilite tub sideways, spray lysol in it, and do my g2g's in there.

I'd recommend a glovebox or a flowhood. My method works great for me however.


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
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Re: make shift sterile environment for GTG [Re: qwon]
    #3396264 - 11/23/04 01:07 AM (12 years, 6 months ago)

You could make a glove box - like you describe. Or, if you have a little hepa filter (or not), simply scrub down your bathroom (as it is small & usually almost all tile - or Formica), remove all cloth, take jars into bathroom, (run filter if you have one - for 15 minutes), spray Lysol all over the place (including your arms & hands), wearing just boxers, a shower cap to cover your hair & a surgical mask. Shut off filter (if applicable), with slow deft movements - do G2G. Worked for this old hand - hundreds of times. Then - upgrade to a flow/hood (if applicable).















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Offlinemushroommark
Earning mybluethumb

Registered: 10/01/04
Posts: 359
Last seen: 10 years, 9 months
Re: make shift sterile environment for GTG [Re: agar]
    #3396271 - 11/23/04 01:09 AM (12 years, 6 months ago)

What's the little black piece on top of the uncolonized jar lid??


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Invisibleagar
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Re: make shift sterile environment for GTG [Re: mushroommark]
    #3396339 - 11/23/04 01:24 AM (12 years, 6 months ago)

PC proof grommet that holds a tyvek filter membrane in place (on both inside & exterior) over ? inch hole in lid..



You can build all sorts of lids.

Here is one with an exterior filter & injection port



Here is a simple one, with injection port



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Offlineqwon
destroying angel
Registered: 08/21/04
Posts: 324
Last seen: 4 years, 2 months
Re: make shift sterile environment for GTG [Re: agar]
    #3396987 - 11/23/04 05:25 AM (12 years, 6 months ago)

you guys are the bomb.  I'm doin it when the lady leaves town today for thanksgiving.  can't wait! :thumbup: :thumbup: :thumbup: :thumbup: :thumbup:


--------------------
'I'm the commander see, I don't need to explain, I do not need to
explain why I say things. That's the interesting thing about being the president.
Maybe somebody needs to explain to me why they say something,
but I don't feel like I owe anybody an explanation.'
- George W. Bush


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Offlinebeejay
some randominternet dude

Registered: 10/03/04
Posts: 2,601
Loc: The Dark Tower
Last seen: 6 years, 10 months
Re: make shift sterile environment for GTG [Re: agar]
    #3397410 - 11/23/04 10:15 AM (12 years, 6 months ago)

Hey Agar, Ive been wanting to make some injector site lids. Only problem is I cant find any self healing inector material. Where'd ya get it? You should write a tek on making specialty lids. I think a lot of people would be interested in your methods. Especially me.  :thumbup:


--------------------
Anjaba said:
Oh shit, don't drink it.... It would eat away your esophagus...
mantis said:
Leave me out of this pissing contest, you fascist wang-dang-doodle!
Hattori Hanzo said:
If on your journey, you should encounter God, God will be cut


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Offlinebeejay
some randominternet dude

Registered: 10/03/04
Posts: 2,601
Loc: The Dark Tower
Last seen: 6 years, 10 months
Re: make shift sterile environment for GTG [Re: beejay]
    #3405332 - 11/24/04 08:07 PM (12 years, 6 months ago)

bump, I need to know where to get these self healing injector sites.


--------------------
Anjaba said:
Oh shit, don't drink it.... It would eat away your esophagus...
mantis said:
Leave me out of this pissing contest, you fascist wang-dang-doodle!
Hattori Hanzo said:
If on your journey, you should encounter God, God will be cut


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 3 years, 1 month
Re: make shift sterile environment for GTG [Re: beejay]
    #3405396 - 11/24/04 08:26 PM (12 years, 6 months ago)

bliggiddy blam, and this took me a few minutes.

http://www.shroomery.org/forums/showflat...rue#Post3393182


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 3 years, 1 month
Re: make shift sterile environment for GTG [Re: 4hodmt]
    #3405406 - 11/24/04 08:28 PM (12 years, 6 months ago)

though u may hav to buy sum more canning lids.

oh well, i thuink theyre cheap.


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: make shift sterile environment for GTG [Re: beejay]
    #3405436 - 11/24/04 08:36 PM (12 years, 6 months ago)



The ones you can PC look like this (above). Search google for "septa plug". They are out there - but hard to find. I bought a bag full a couple years back - from a west coast "surplus" medical supply place, but it isn't in Biz any longer.

Sorry, I don't have an easy ready source - on hand. Just seek & you shall find. Good luck.


--------------------


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Invisibleagar
old hand
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Re: make shift sterile environment for GTG [Re: agar]
    #3405474 - 11/24/04 08:43 PM (12 years, 6 months ago)



--------------------


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 1 month
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Re: make shift sterile environment for GTG [Re: agar]
    #3414024 - 11/26/04 11:27 PM (12 years, 6 months ago)



--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineMadHatR
journeyman
Registered: 10/13/03
Posts: 262
Last seen: 1 day, 13 hours
Re: make shift sterile environment for GTG [Re: hyphae]
    #3414412 - 11/27/04 12:57 AM (12 years, 6 months ago)

Quote:

hyphae said:
Or better yet!




Great site, low prices and a minimum order requirement of only $10. Much better than all the ones I had found in the past that required at-least a $200 order. Especially with the fact that, as we all should know, by law any orders of over $100 in lab equipment has to be reported to the government by the company. How well do those hold up in the PC as far as number of times they can be reused. Will they harden up over time like the PC's O-ring? Just wondering how many I should order so I never run out.


--------------------
Following the rules is for dealers, knowing why the rules work is for innovators.

"Knowing is half the battle." - G.I. Joe

Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 1 month
Trusted Cultivator
Re: make shift sterile environment for GTG [Re: MadHatR]
    #3415639 - 11/27/04 01:40 PM (12 years, 6 months ago)

The PC gasket WILL harden over time IF you use vegetable oil as a conditioner guaranteed, believe me I've went thru a couple already, now it's petroleum jelly and they are as good as the day I got them. Now as far as those septa plugs I couldn't tell ya I'm a bagman now and never really used those YET :wink: I will be incoorperating them into my liquid inoc teks coming up :laugh: GL guys


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineMadHatR
journeyman
Registered: 10/13/03
Posts: 262
Last seen: 1 day, 13 hours
Re: make shift sterile environment for GTG [Re: hyphae]
    #3415992 - 11/27/04 03:45 PM (12 years, 6 months ago)

I to am a bagman, but would like to use them for liquid. Right now I am using those self-molding ear plugs, but they do not last that long and cost more than those plugs.


--------------------
Following the rules is for dealers, knowing why the rules work is for innovators.

"Knowing is half the battle." - G.I. Joe

Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.


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OfflineTantalus
Beyonddescription.
Registered: 06/05/03
Posts: 747
Last seen: 11 years, 9 months
Re: make shift sterile environment for GTG [Re: hyphae]
    #3416042 - 11/27/04 04:05 PM (12 years, 6 months ago)

For you guys that have hepa filters, you should consider making it into a flowhood by working in it's airstream.

AFOAF has a rubbermaid with the bottom cut out. He ducktaped a garbage bag to the bottom, & cut out the bottom of the garbage bag. Whenever he wants to use his hepa as a flowhood, he simply lysols the rubbermaid with the garbage bag taped to it & slips the cut out bottom over the top of his hepa so that nothing but filtered air blows through the rubbermaid. He's left open cultures in the airstream sometimes for days without contam.


--------------------
"A nation's hope of lasting peace cannot be firmly based upon any race in armaments but rather upon just relations and honest understanding with all other nations...

Every gun that is made, every warship launched, every rocket fired signifies, in the final sense, a theft from those who hunger and are not fed, those who are cold and are not clothed... The cost of one modern heavy bomber is this: a modern brick school in more than 30 cities. We pay for a single fighter with a half million bushels of wheat. We pay for a single destroyer with new homes that could have housed more than 8,000 people..."

President Dwight Eisenhower, 1953


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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Rye Grain

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