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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Idiot proof spore syringe
    #3389568 - 11/20/04 04:54 PM (12 years, 15 days ago)

Cut two 1/4 inch strips of plastic off the end of a spawnbag make them about 3 inches long.

Throw them into a container of your favorite sterilizer peroxide bleach/water alcohol whatever,let soak.

Clean a section of bench or a plate with same.

Pull out the strips with sterile tweezers and let them dry on the plate under a clean  sterile glass.

Put your cap on the plate and put the strips under it like so.
don't let the strips touch the stem.

let the spores fall till they are nice and thick.

Pick up the strips with STERILE tweezers and drop them into sterile water and shake....suck up and let it sit in the syringe for 12 hours and use as normal.

for a really dark syringe pull the plunger out of the syringe and put the strips inside it, put plunger back in and draw in water.

Once in a while the plastic may block the needle when you're injecting but if you stick a sterilized pipe screen in that is a snug fit into the syringe beforehand it will keep the plastic strip from blocking the needle.you could also just draw back a bit and tap it then inject again....your call.

I haven't tried sterilizing a needle with a screen in it in the pressure cooker so do so at your own risk..it may melt into the syringe body and ruin it,I'm not sure.

This also works with those little 1cc and 2 cc syringes you can get at the pharmacy but you have to make the strips smaller.

It's also a good method for getting morel spores or any other fruitbody without a stem that you have to hang just tape it to the top of the glass or lay it over two pencils that have the strips between them.

Lather,rinse repeat.:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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InvisibleSuper_Blunt
Candyman
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Registered: 10/25/04
Posts: 3,140
Re: Idiot proof spore syringe [Re: Peterthinks]
    #3389579 - 11/20/04 04:56 PM (12 years, 15 days ago)

nice


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OfflineIGnosticAbhorI
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Registered: 11/06/04
Posts: 4,898
Last seen: 1 year, 27 days
Re: Idiot proof spore syringe [Re: Super_Blunt]
    #3389586 - 11/20/04 04:59 PM (12 years, 15 days ago)

Good idea, but fool proof? you know there's got to be aleast one dumbass who will acually Fuck it up. But it sounds like a nice tek to me. I'll surely give it a try! gl


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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: Idiot proof spore syringe [Re: IGnosticAbhorI]
    #3389935 - 11/20/04 06:21 PM (12 years, 15 days ago)

Yeah somebody is bound to mess it up.
but if they stick to the directions it should work out.
just make sure you soak the strips in saliva and shit....no I mean isopropyl alcohol
and if you take out the plunger only put it down on something sterile...like a flamed petri dish or alcohol soaked cotton sheet.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Edited by Peterthinks (11/20/04 06:23 PM)


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 2 years, 6 months
Re: Idiot proof spore syringe [Re: Peterthinks]
    #3389950 - 11/20/04 06:24 PM (12 years, 15 days ago)

i think an easier techinigue is simply sterilize a jar, then set the mushroom inside it. put the lid on. open it in a glovebox or outside brriefly, then remove the cap. inject water, and uve got some nice spore solution.


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


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OfflineIGnosticAbhorI
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Re: Idiot proof spore syringe [Re: 4hodmt]
    #3389955 - 11/20/04 06:26 PM (12 years, 15 days ago)

4hodmt, yeah that is a bit easier but to each his own.


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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: Idiot proof spore syringe [Re: IGnosticAbhorI]
    #3389963 - 11/20/04 06:28 PM (12 years, 15 days ago)

Yeah but if you put it in the jar it's touching the jar.
surface bacteria on the shroom


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
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Re: Idiot proof spore syringe [Re: Peterthinks]
    #3389969 - 11/20/04 06:30 PM (12 years, 15 days ago)

so dip it in a little peroxide. this is a tried and true method;)....


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler


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OfflineCrabbyAss
sharp shooter

Registered: 06/02/02
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Re: Idiot proof spore syringe [Re: Peterthinks]
    #3389981 - 11/20/04 06:35 PM (12 years, 15 days ago)

Good tek.  Definitly has its benefits. Excellent info.

Some slight variations I have heard are as follows:

use a sterile gauze pad cut into 4 parts. Print directly on gauze, than tweeze gauze parts into water, karo, spawn bag, ect.

This type of printing eliminates the need for scraping, and ensures all the spores make it to the correct destination. 

Holding an index card over your oven while your scraping microscopic spores isn't a pretty sight. But to take tweezers and grab and drop takes less than 3 seconds of exposure time, as opposed to the 30+ seconds of scraping, mixing, and sucking.

thumbs up  :thumbup:


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InvisibleGnuBobo
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Re: Idiot proof spore syringe [Re: 4hodmt]
    #3390037 - 11/20/04 06:55 PM (12 years, 15 days ago)

Quote:

4hodmt said:
so dip it in a little peroxide. this is a tried and true method;)....





Do you mean submerge the entire cap (and gills) in straight 3% peroxide for a couple of seconds then removing it? Or swabbing down the top of the cap with straight peroxide? Will spores drop after the gills get wet?


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: Idiot proof spore syringe [Re: CrabbyAss]
    #3390044 - 11/20/04 06:56 PM (12 years, 15 days ago)

I've also done this with a sterile washer.
the washer is in black.
note the mushroom is off center so more gills are over the opening so the spores drop inside more

it's in a tall glass stuck into playdough.(the cover is still on the needle)
after you just put the plunger in and draw water into it when you want to use it.
you can keep the spores inside it dry for as long as you like!
I call it a dry syringe.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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OfflineFerment
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Registered: 11/10/04
Posts: 99
Loc: NE USA
Last seen: 10 years, 5 months
Re: Idiot proof spore syringe [Re: Peterthinks]
    #3390081 - 11/20/04 07:05 PM (12 years, 15 days ago)

Quote:

Peterthinks said:
I've also done this with a sterile washer.
the washer is in black.
note the mushroom is off center so more gills are over the opening so the spores drop inside more

it's in a tall glass stuck into playdough.(the cover is still on the needle)
after you just put the plunger in and draw water into it when you want to use it.
you can keep the spores inside it dry for as long as you like!
I call it a dry syringe.




I've never taken spore prints so perhaps I'm not one to talk, but why are you putting an entire cap's worth of spores into one syringes? I've repeatedly seen it said that 1 decent sized cap can be made into 5-10 syringes.


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Offlinelonebuddha
whats it allabout?
Registered: 11/01/04
Posts: 255
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Re: Idiot proof spore syringe [Re: GnuBobo]
    #3390108 - 11/20/04 07:11 PM (12 years, 15 days ago)

I thought the spores would be killed by the h2o2.


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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: Idiot proof spore syringe [Re: Ferment]
    #3390121 - 11/20/04 07:16 PM (12 years, 15 days ago)

Well I'm working with food mushrooms.if it takes 5 mushrooms to make 5 syringes it's OK
I'm more concerned with how contaminant free it is.
I could inject one syringe into 5 times the water and fill 5 syringes but I only need one at a time.
I'm just sharing ideas,if it isn't what you need for what you do just ignore it or adapt it.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: Idiot proof spore syringe [Re: Peterthinks]
    #3583849 - 01/05/05 01:33 PM (11 years, 10 months ago)

bump to share the spore thing


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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Invisiblemadsickpenguin
newbie
Registered: 08/29/04
Posts: 234
Re: Idiot proof spore syringe [Re: GnuBobo]
    #3584532 - 01/05/05 04:48 PM (11 years, 10 months ago)

Quote:

GnuBobo said:
Quote:

4hodmt said:
so dip it in a little peroxide. this is a tried and true method;)....





Do you mean submerge the entire cap (and gills) in straight 3% peroxide for a couple of seconds then removing it? Or swabbing down the top of the cap with straight peroxide? Will spores drop after the gills get wet?




yes please explain


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