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Offlinespliffmasta
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How do I know when my liquid culture is ready to use?
    #3381352 - 11/18/04 09:39 PM (13 years, 6 months ago)

How much myc should I wait for?


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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: spliffmasta]
    #3381372 - 11/18/04 09:43 PM (13 years, 6 months ago)

wait untill you got a good LITTLE cotton ball growing and if you shake early look for it to look like someone spit into the jar.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineIGnosticAbhorI
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3381406 - 11/18/04 09:53 PM (13 years, 6 months ago)

wow...thats the nastiest description of something good ive ever heard...gross but now i kno when to inoculate : )


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Offlinediscman1
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Re: How do I know when my liquid culture is ready to use? [Re: IGnosticAbhorI]
    #3381420 - 11/18/04 09:57 PM (13 years, 6 months ago)

This was after 2 weeks, and it was enough to make 8 syringes.

Sucking the balls up and spitting them back out produced huge clouds of myc, the water was milky.




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OfflineShdwstr
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3381942 - 11/18/04 11:44 PM (13 years, 6 months ago)

Quote:

lemon_lw said:
wait untill you got a good LITTLE cotton ball growing and if you shake early look for it to look like someone spit into the jar.




Or... you could wait till it looks like this  :shocked:



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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3381948 - 11/18/04 11:46 PM (13 years, 6 months ago)

damn shdwstr how long did it take to get like that?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineShdwstr
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3381969 - 11/18/04 11:55 PM (13 years, 6 months ago)

That is a 7 day old Malaysian Jar made with Light Malt.


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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3381990 - 11/19/04 12:00 AM (13 years, 6 months ago)

wow mine looked nothing like that at 7 days whats your secret?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinespliffmasta
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3382025 - 11/19/04 12:08 AM (13 years, 6 months ago)

That's insane for 7 days! Maybe that malt stuff is the way to go :smile:


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OfflineShdwstr
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3382031 - 11/19/04 12:08 AM (13 years, 6 months ago)

No secret. That strain just worked well. Some don't.
I never did get an Orrisa to work as a liquid culture.


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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3382034 - 11/19/04 12:09 AM (13 years, 6 months ago)

thats cool looks perty. how did it grow?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineShdwstr
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3382072 - 11/19/04 12:21 AM (13 years, 6 months ago)

Perfectly  :cool:


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Offlinespliffmasta
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3382076 - 11/19/04 12:22 AM (13 years, 6 months ago)

Was it noc'd up itself with liquid culture? Now that would make a lot more sense.


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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: spliffmasta]
    #3382086 - 11/19/04 12:23 AM (13 years, 6 months ago)

good shit did you recognize any increase with colonization time with it or any thing that was better and what is this malt you speak of.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineShdwstr
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Re: How do I know when my liquid culture is ready to use? [Re: lemon_lw]
    #3382253 - 11/19/04 01:09 AM (13 years, 6 months ago)

Jar was innocculated from a spore syringe (1cc)
Colonization time saves about 5 - 7 days over multispore injection.

The malt is dry malt powder, available at any U-brew (beer) store.

Stick to 4% solutions.
eg.

For Malt Liquid culture - 1tb.(10g) Malt to 1 Cup(250ml) water
For Agar Solid Culture - 1 tb.(10g) Malt + 1tb.(10g) Agar to 2 Cups(500ml)

You can also replace the dry malt with dextrose sugar from a drug store, health food store etc. It won't work as well in the agar mix though.


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Offlinelemon_lw
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3383088 - 11/19/04 09:05 AM (13 years, 6 months ago)

sounds like regular karo will ultimatly do te same as to speeding up colonizing time. so ill just stick with what i know


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineFungi_x
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Re: How do I know when my liquid culture is ready to use? [Re: Shdwstr]
    #3385360 - 11/19/04 06:30 PM (13 years, 6 months ago)

Quote:

Shdwstr said:
No secret. That strain just worked well. Some don't.
I never did get an Orrisa to work as a liquid culture.




I have had alot of issues with BHT in kero


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Who cares what pubbers say -notapillow
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