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Offlineballs
thats right
Registered: 11/01/04
Posts: 346
Loc: Ontario, Canada
Last seen: 17 years, 3 months
Inoculating cold jars
    #3368351 - 11/16/04 07:38 PM (18 years, 15 days ago)

I took my jars out of the fridge this morning, and they have been sitting at room temperature for about 2 hours now. I can still feel a little cold, but is it okay to inoculate now?


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Offlinedaimyo
Monticello

Registered: 05/13/04
Posts: 7,751
Last seen: 10 years, 9 months
Re: Inoculating cold jars [Re: balls]
    #3368357 - 11/16/04 07:40 PM (18 years, 15 days ago)

Yes


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"I have sworn upon the altar of God eternal hostility against every form of tyranny over the mind of man."


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Offlinelemon_lw
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Registered: 10/18/04
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Re: Inoculating cold jars [Re: balls]
    #3368358 - 11/16/04 07:40 PM (18 years, 15 days ago)

dude i inoculated some jars that were cold as shit strait out of the fridge about 5 days ago and they are doing awesome.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlineballs
thats right
Registered: 11/01/04
Posts: 346
Loc: Ontario, Canada
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Re: Inoculating cold jars [Re: lemon_lw]
    #3368364 - 11/16/04 07:42 PM (18 years, 15 days ago)

rad


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OfflineIce House Shaman
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Re: Inoculating cold jars [Re: balls]
    #3368378 - 11/16/04 07:47 PM (18 years, 15 days ago)

Cold inoculation will work just fine, cold incubation is even better. Slower, but, if you are not in a hury, your myco will be stronger healthier and it will not contam. Cold incubated myco grows out slowly in mass very dense. Thats the way 2 go


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you are not who i thought i was...


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Offlinelemon_lw
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Re: Inoculating cold jars [Re: Ice House Shaman]
    #3368390 - 11/16/04 07:49 PM (18 years, 15 days ago)

so thats why my mycelium in my older jars was alot thicker than my newer jars. when i first started colonizing i didnt have a heat source for about a week and they are thick i tell you.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinedeeproots
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Registered: 11/05/04
Posts: 35
Last seen: 17 years, 1 month
Re: Inoculating cold jars [Re: lemon_lw]
    #3369313 - 11/16/04 11:48 PM (18 years, 15 days ago)

"Cold inoculation will work just fine, cold incubation is even better. Slower, but, if you are not in a hury, your myco will be stronger healthier and it will not contam. Cold incubated myco grows out slowly in mass very dense. Thats the way 2 go"

Why is that the way to go, higher yield? Mine are growing in a 65 degree room and its taking forever!!!!!!!!!!!!!!


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Offlinecatchmikeny
Stranger
Registered: 11/19/04
Posts: 8
Last seen: 18 years, 11 hours
Re: Inoculating cold jars [Re: deeproots]
    #3381413 - 11/19/04 04:55 AM (18 years, 13 days ago)

cold incubation is basically using no heat and just letting them colonize at room temp?


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OfflineVagrant
Fun Guy

Registered: 10/04/04
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Loc: earth
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Re: Inoculating cold jars [Re: catchmikeny]
    #3381476 - 11/19/04 05:11 AM (18 years, 13 days ago)

I've had much better luck with warm incubation than cold. Cold had much bigger ratio of contams.


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Offlinelemon_lw
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Re: Inoculating cold jars [Re: Vagrant]
    #3381504 - 11/19/04 05:16 AM (18 years, 13 days ago)

alright i just got a little thought. what if you start with warm inncubation and after about half way colonized you change to cold. i would think it would help to reduce the chances of contams cause the mycelium has already got a footing. and at the same time it seems you could benifit still from the cold inncubation. just a thought i know its not really that great of one but it might help out.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offline13eetleJuice
the ghost with the most
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Re: Inoculating cold jars [Re: lemon_lw]
    #3381593 - 11/19/04 05:30 AM (18 years, 13 days ago)

But isnt temperature (or a lowering of thereof) one of the pinning triggers the mycelium uses to know when to start producing fruit? If you incubate them at the same temp that you plan to keep them at when transferred to the fruiting chamber then it seems you have lost one of your triggers. Am I way off on this?

I know light is another trigger. So I suppose if you incubated them in a dark place then introduced them to lots of light, they would begin to pin just fine. I dunno. Have any of you guys that cold incubate your jars had any problems with this at all?


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Offlinelemon_lw
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Re: Inoculating cold jars [Re: 13eetleJuice]
    #3381624 - 11/19/04 05:35 AM (18 years, 13 days ago)

i get what your saying and im no expert so the question to someone who is more experienced. is a drop from 86F to around 76F enought to cause the cakes to want to pin or would it have a negative effect on cold shocking it in the future?


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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