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Amazon Shop for: ½ Pint Jars, Paul Stamets, Rye Grain

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OfflineRootedGrowth
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G2G questions
    #3367321 - 11/16/04 02:38 AM (12 years, 21 days ago)

Ok, so my understanding of G2G is to speed up the colonization process without the need for prints or syringes. Is this correct?

Also, if I do G2G, do I lose anything over generations? Would it be better to inoculate via a syringe after a set number of generations?

I am looking for the quickest way to mass produce I suppose. So should I even be considering G2G or should I just use a rye tek and inoculate with a mycelium culture(perhaps honey or karo tek)?

Any help or suggestions are sincerely appreciated.


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Offlinebuckeye
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Re: G2G questions [Re: RootedGrowth]
    #3367338 - 11/16/04 02:43 AM (12 years, 21 days ago)

yes
Yes, strains do weaken over generations. It is good to start from a spore print every 2-2 generations.
G2g would help, along with the karo tek.


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OfflineRootedGrowth
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Re: G2G questions [Re: buckeye]
    #3367343 - 11/16/04 02:46 AM (12 years, 21 days ago)

"G2g would help, along with the karo tek. "

I am not sure what you mean here. Does this mean using G2G or Karo tek I could go more than 2 - 3 generations?

Is my understanding of G2G correct?

Does the strain weaken over generations using karo tek as well?


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Offlinebuckeye
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Re: G2G questions [Re: RootedGrowth]
    #3367361 - 11/16/04 02:51 AM (12 years, 21 days ago)

They both are good methods of producing a lot of substrate.

depends how you use the karo tek. If you make up a batch of karo, and then let it colonize and then make up more karo and inoculate the new karo with the old karo, then you will lose over generations.
if you make up a batch of karo with spores and use all that karo, then make up another karo and innoc with spores again, you will not lose anything.

Your understanding is correct.

Yes see above.


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Offlinedmc_
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Re: G2G questions [Re: RootedGrowth]
    #3367368 - 11/16/04 02:55 AM (12 years, 21 days ago)

G2G is the concept of expanding your spawn culture in an exponential fashion.

[qoute]I am looking for the quickest way to mass produce I suppose.




Which does fit what you're looking for.

You then take a set number of each of these jars and add to a bulk.

Thats about as quick and bulk as you can get.


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OfflineRootedGrowth
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Re: G2G questions [Re: dmc_]
    #3367409 - 11/16/04 03:13 AM (12 years, 21 days ago)

I have heard all you need is about a tablespoon of colonized substrate to inoculate another quart jar? Is this accurate?

Obviously the more used, the faster it will colonize the new jar.

What is a conservative figure for the number of jars you can inoculate with one fully colonized grain quart-sized jar?

Thanks again for all the info.


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OfflineMadHatR
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Re: G2G questions [Re: dmc_]
    #3367429 - 11/16/04 03:24 AM (12 years, 21 days ago)

Both the karo and G2G are basically the same concept. One is myc growing suspended in water and the other has it growing right on grain. So both have the same effect and limitations. As far as generations, from what I understand and correct me someone if I am wrong, but it is truly not a generations issues. Any transfer risks contamination and pretty much amplifies any flaws that already exists in the DNA of the strain. So transferring and then transferring from that transfer in a sense weakens it through exposure. Both Liquid and G2G have their pluses. Liquid does not last as long, a fully colonized grain jar can be stored in the fridge for a very long time and then used. Liquid usually can be done safely without investing in a glovebox or flowhood though. G2G does seem to colonize a bit faster though. In my opinion it is best to use both. Use liquid to make 100 or so master jars or oven bags with 2 liter tops inoc points. While you are waiting build a glove box then start using the jars or bags you made to spawn jars.

Mass production really should not be the goal though. This is a hobby, not a business. Something that is done for fun, and spiritual awareness of you and your friends. People are bound to ask though, every week or so it seems. :smile:


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Following the rules is for dealers, knowing why the rules work is for innovators.

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Offlinepshawny
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Re: G2G questions [Re: RootedGrowth]
    #3367471 - 11/16/04 04:02 AM (12 years, 21 days ago)

One jar will be enough to do g2g to 10 jars of the same size. So you could take one jar and end up with 1000 in a short period of time given contams are kept to a minimum. GL


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OfflineRootedGrowth
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Re: G2G questions [Re: pshawny]
    #3367519 - 11/16/04 04:49 AM (12 years, 21 days ago)

I don't think I will need a thousand.  :smile:

Just wanted to make enough for a lot of friends before xmas! 

Thanks for the info guys


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InvisibleKyKid
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Re: G2G questions [Re: RootedGrowth]
    #3367800 - 11/16/04 09:36 AM (12 years, 21 days ago)

well you better get on it then if you want it before christmas. good luck.......


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OfflineKaptKid
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Re: G2G questions [Re: KyKid]
    #3368230 - 11/16/04 12:06 PM (12 years, 21 days ago)

1 qt can make 10 qt's doing g2g according to TMC


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Amazon Shop for: ½ Pint Jars, Paul Stamets, Rye Grain

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