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InvisiblePinhead
Oregano
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Registered: 05/14/00
Posts: 1,819
Loc: Hootersville
Oxytetracycline HCL
    #336607 - 06/07/01 07:08 AM (21 years, 3 months ago)

I want to experiment using antibiotics in agar,it just so happens that I have quite a bit of Oxytetracycline HCL left over from my Y2K supplies. I know that some antibiotics, like amoxicillin are heat sensitive and have to be added after pc'ing...what about tetracycline?



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Invisiblepaddoholland
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Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Oxytetracycline HCL [Re: Pinhead]
    #336823 - 06/07/01 03:27 PM (21 years, 3 months ago)

This will be damaged also (I think), because in our lab we cold sterilize the antibiotic (push through a filter) and put it in the agar jar after sterilisation. Be very carefull with oxytetracyclyne it's very heavy shit.

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Invisiblepaddoholland
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Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Oxytetracycline HCL [Re: Pinhead]
    #336826 - 06/07/01 03:28 PM (21 years, 3 months ago)

Why are you using antibiotics, you can't tell if there are bacteria present because they don't grow! So you son't know if your agar is contaminated!
btw maybe a stupid question, Y2K (I always see this related 2 ganja) what does Y2K mean????

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Edited by paddoholland on 06/07/01 08:30 AM.



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Offlinegray1
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Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 20 years, 5 months
Re: Oxytetracycline HCL [Re: paddoholland]
    #336851 - 06/07/01 04:25 PM (21 years, 3 months ago)

definately add oxytet after autoclaving/pressure cooking, it, like most antibiotics, is heat sensitive.
paddoholland has a point in that some antibiotics inhibit growth but don't entirely kill all bacteria that are present and so in liquid culture with which you intend to innoculate solid substrate it could be a waste of substrate if it turns out you had contamination but i think that this scenario would be unlikely, and for agar propogation it is a good idea to include antibiotics especially if you are using this technique to store/perpetuate cultures as they will be around and potentially exposed to infection for long periods of time

year two thousand
assuming that you're from holland or elsewhere abroad you are fortunate not to have been innundated with this catch phrase that has been so overused that the mere sounding of its syllables invokes painful annoyance.





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InvisiblePinhead
Oregano
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Registered: 05/14/00
Posts: 1,819
Loc: Hootersville
Re: Oxytetracycline HCL [Re: gray1]
    #336911 - 06/07/01 05:31 PM (21 years, 3 months ago)

Thanks for the info. I do keep cultures in the fridge in agar,so maybe I'll include antibiotics.The year 2000 (Y2k) was supposed to bring with it massive computer crashes,etc,etc.
and so on.



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Invisiblepaddoholland
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Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Oxytetracycline HCL [Re: gray1]
    #337486 - 06/08/01 08:40 AM (21 years, 3 months ago)

hmmm year 2 thousand. thanks

I really don't believe your antibiotics will kill all contams on agar. I suppose if you seal your dishes with foil they won't get contaminated.

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Edited by paddoholland on 06/08/01 01:41 AM.



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Offlinemadscientist
journeyman
Registered: 05/26/01
Posts: 110
Last seen: 20 years, 1 month
Re: Oxytetracycline HCL [Re: paddoholland]
    #337552 - 06/08/01 10:20 AM (21 years, 3 months ago)

tetracycline will KILL almost all bacteria but wont do much to fungal contams which are the real problemo hear.



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