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Offlinedewey
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Registered: 05/26/04
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Last seen: 12 years, 12 days
Germination in Liquid Cultures?
    #3360942 - 11/14/04 07:09 PM (12 years, 21 days ago)

From a previous post it seems i have a germinated (or germinating) spores) within my spore needles.  The mass of germinating spores seem to be getting larger within my liquid culture.  From what i have read it should be a whites fluffy mass but since mine are germinating it has a dark grey color.  Will my liquid culture still be allright considering germination has taken place?  Will they last 6 months in the fridge when after i have inoculated my pf cakes?

Info & help appreciated!!

dewey :mushroom2:


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Offlinelemon_lw
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Re: Germination in Liquid Cultures? [Re: dewey]
    #3360971 - 11/14/04 07:15 PM (12 years, 21 days ago)

hello dewey. i have a quick little question for you. did you heat your needle before innoc? if i saw some fuzzy stuff i would either use a different needle or at least flame it.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinedewey
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Re: Germination in Liquid Cultures? [Re: lemon_lw]
    #3361018 - 11/14/04 07:24 PM (12 years, 21 days ago)

Yes first i had a few spore prints and created the needles using sterile needles, distilled water and a sterile working environment. The culture produced many a fine crop. Now after being in the fridge for 2 months the needles with the keepers creepers have light frey flakey masses in them whereas the blue meanie syringes i have don't. In a previous post someone said that that flayey mass was germinated spores floating in the culture. I am still looking for a second opinion1

dewey


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Offlinelemon_lw
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Re: Germination in Liquid Cultures? [Re: dewey]
    #3361067 - 11/14/04 07:35 PM (12 years, 21 days ago)

ok i have done a couple of liquid culters and mine are perfectly white untill shook up and then it looks like spit. one way to figure out is to whip up a couple of jars and see what happens


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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