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OfflineMushroomFriend
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Shake your thing!
    #3354941 - 11/13/04 10:55 AM (12 years, 8 months ago)

Hello dudes and dudettes,

Since my incubation is going very slow I wonder some things.
One of them is if people shake there syringe before inoculation!

If you would be so kind to vote?

Thanks!
Do you shake your syringe before you inoculate?
You may choose only one
Yes, shaking is good, I shake my syringes before inoculation.
No, I dont shake them it is no use.


Votes accepted from (11/13/04 10:55 AM) to (No end specified)
View the results of this poll



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Edited by MushroomFriend (11/13/04 11:06 AM)


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OfflineMushroomFriend
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Re: Shake your thing! [Re: MushroomFriend]
    #3354942 - 11/13/04 10:56 AM (12 years, 8 months ago)

that first vote is mine since i did not shake my syringe the first (and only) time I used it.


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Invisibledobinky
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Re: Shake your thing! [Re: MushroomFriend]
    #3354947 - 11/13/04 10:59 AM (12 years, 8 months ago)

shaking it breaks up clusters of spores in the water, but you would have success either way.


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OfflineMushroomFriend
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Re: Shake your thing! [Re: dobinky]
    #3354955 - 11/13/04 11:04 AM (12 years, 8 months ago)

I found my syringe very clear, not purplish or something.
I think shaking would spread them more equal in the syringe.

Now some jars havent shown any colonizing at all yet and its been 8 days now. The biggest white spot is the the size of a female thumbnail.. :wink:

In the popcorn there is one bigger colonized part...... The popcorn is more humid too, maybe that is the biggest cause of slow growth>


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Invisibledobinky
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Re: Shake your thing! [Re: MushroomFriend]
    #3354960 - 11/13/04 11:06 AM (12 years, 8 months ago)

hhmm well when the popcorn gets about 30% colonized... shake it fast...


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Offlinehungstruggling
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Re: Shake your thing! [Re: MushroomFriend]
    #3354963 - 11/13/04 11:08 AM (12 years, 8 months ago)

I shake only 3 times, because anymore and you are playing with it.


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Invisibleohmatic
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Re: Shake your thing! [Re: hungstruggling]
    #3355037 - 11/13/04 11:45 AM (12 years, 8 months ago)

well, shake syringe with plunger on, remove plunger, shoot up.
doing it that way u distribute the spores more than just shooting directly
which can lead to enormous masses of spores in one jar and way less concentrated
spores in others.

by shaking u even it out a bit.
peace ohm :mushroom2:


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OfflineMushroomFriend
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Re: Shake your thing! [Re: dobinky]
    #3355128 - 11/13/04 12:13 PM (12 years, 8 months ago)

Quote:

dobinky said:
hhmm well when the popcorn gets about 30% colonized... shake it fast...




Yeah i will shake it!


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OfflineMushroomFriend
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Re: Shake your thing! [Re: ohmatic]
    #3355132 - 11/13/04 12:14 PM (12 years, 8 months ago)

untill now it seems like everyone is shaking their thingy! :laugh:


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Invisibledobinky
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Re: Shake your thing! [Re: MushroomFriend]
    #3355143 - 11/13/04 12:16 PM (12 years, 8 months ago)

i think only the men can shake their thingy....you woman must shake your thang....heh it sounds like it makes sense to me....


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OfflineMushroomFriend
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Re: Shake your thing! [Re: dobinky]
    #3355173 - 11/13/04 12:24 PM (12 years, 8 months ago)

Quote:

dobinky said:
i think only the men can shake their thingy....you woman must shake your thang....heh it sounds like it makes sense to me....




Ur right i am being sexistic presuming only men shake! Women have more to shake even, so babes shake your thungS! :laugh:


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Offlinecall_me_kido
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Re: Shake your thing! [Re: MushroomFriend]
    #3355217 - 11/13/04 12:38 PM (12 years, 8 months ago)

If its a PF tek, give it some time. After two weeks start to worry.

Kido


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"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle


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OfflineMushroomFriend
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Re: Shake your thing! [Re: call_me_kido]
    #3355251 - 11/13/04 12:50 PM (12 years, 8 months ago)

Yeah its a PF tek.... Ahh im being impatient i know :frown:

Buttt do you shake your thing ? :laugh:


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OfflineK20A2
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Re: Shake your thing! [Re: MushroomFriend]
    #3355273 - 11/13/04 12:57 PM (12 years, 8 months ago)

I shake the syringe.


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Invisiblewoodzmun
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Re: Shake your thing! [Re: K20A2]
    #3355321 - 11/13/04 01:12 PM (12 years, 8 months ago)

I hate to say it MF but there's your trouble. No shakey means some jars may have not gotten any spores. If you got another syringe shake it, and inoculate the jars with no growth.GL.


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Offline4hodmt
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Re: Shake your thing! [Re: woodzmun]
    #3355593 - 11/13/04 02:18 PM (12 years, 8 months ago)

i use karo. its better.


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Invisiblewoodzmun
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Re: Shake your thing! [Re: 4hodmt]
    #3355658 - 11/13/04 02:32 PM (12 years, 8 months ago)

Agreed, if ya got it by all means.


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Offlinedebianlinux
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Re: Shake your thing! [Re: woodzmun]
    #3355814 - 11/13/04 03:19 PM (12 years, 8 months ago)

when using liquid culture it is absolutely essential to shake and frequently. 1stly to tear it up from being one clump and 2ndly to combat the effects of gravity. it can be rather disappointing to watch the entire mycelial contents of a 10ml syringe get ejected in the 1st 2ml.

with spores an inital shaking is highly recommended. using a dispersant (jet dry) helps tremendously as well.


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InvisiblePrisoner#1
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Re: Shake your thing! [Re: debianlinux]
    #3355842 - 11/13/04 03:28 PM (12 years, 8 months ago)

Quote:

debianlinux said:
with spores an inital shaking is highly recommended. using a dispersant (jet dry) helps tremendously as well.




but not one added after the syringe is made...


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OfflineMushroomFriend
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Re: Shake your thing! [Re: woodzmun]
    #3356009 - 11/13/04 04:09 PM (12 years, 8 months ago)

This week ill get another syringe but it will be a different strain. BAd idea inoculating existing jars with it?


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