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Offlinesmoorhsmmm
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help.. i got contam..
    #3353249 - 11/12/04 08:58 PM (12 years, 29 days ago)

hi a few days ago i posted a question about contamination in my jars. i was informed that i probably was using sterile enough techniques. anyway i was wondering why would the contamination only be where i injected the solution and no where else in the jar? does this mean my spore solution was contaminated already? also there were No signs of myc. after about 5-6 days. i pc'd the jars for an hr@ 15 psi, then i prepared a closet by spraying it with lysol and running a vent fan, i also did the flame syringe technique, and after doing at least 10 jars i have nothing to show. what should i do next, i have enough spore solution for about 1-2 jars.!


Edited by smoorhsmmm (11/12/04 09:01 PM)


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Offlinelemon_lw
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353258 - 11/12/04 09:02 PM (12 years, 29 days ago)

just a curious question, what strain was it?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinelemon_lw
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Re: help.. i got contam.. [Re: lemon_lw]
    #3353262 - 11/12/04 09:03 PM (12 years, 29 days ago)

also did you suck any air into the syringe to shake it up?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflinepsilocybinjunkieM
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353263 - 11/12/04 09:04 PM (12 years, 29 days ago)

U didn't pc the jars after you inocculated them did u?
Your syringe is contaminated if the contams are directly located at the injection spots... There would be no other way all the injection sites in your jars would all spontaneously be contaminated if it wasn't the syringe


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Offlinesmoorhsmmm
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Re: help.. i got contam.. [Re: lemon_lw]
    #3353265 - 11/12/04 09:05 PM (12 years, 29 days ago)

i got ecuadorian strain, no there was an airbubble already in the syringe, and i got the syringes from hawkseye! i used the pf tek as my guide. i pc'd the jars and then let them cool over 12 hrs, then i inoculated the jars. check the pic, as u can see the blob is where the solution spread in the jar


Edited by smoorhsmmm (11/12/04 09:12 PM)


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OfflinepsilocybinjunkieM
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353280 - 11/12/04 09:12 PM (12 years, 29 days ago)

what is that? it didn't look like that immediatly after injection did it???


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Offlinesmoorhsmmm
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Re: help.. i got contam.. [Re: psilocybinjunkie]
    #3353285 - 11/12/04 09:13 PM (12 years, 29 days ago)

no that green blob is after about 5 days of incubation.


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OfflineIGnosticAbhorI
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Re: help.. i got contam.. [Re: psilocybinjunkie]
    #3353294 - 11/12/04 09:15 PM (12 years, 29 days ago)

Junkie's right, if it's at All of the injection sites then its your needle, : / , sry about your bad luck but i hope you next try isnt full of Shitty surprizes.


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OfflinepsilocybinjunkieM
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Re: help.. i got contam.. [Re: IGnosticAbhorI]
    #3353303 - 11/12/04 09:18 PM (12 years, 29 days ago)

You got a contaminated syringe... Hawk is good about replacing syringes and backs all of his products. He is kick ass email him an tell him about it he'll replace it for you.
The worst part of this is gonna be the wasted time and effort but rest assured it happens to all of us :beer:
Your jars look great besides the contams :hellfire:


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Offlinesmoorhsmmm
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Re: help.. i got contam.. [Re: psilocybinjunkie]
    #3353312 - 11/12/04 09:21 PM (12 years, 29 days ago)

i know hawks is trying to keep it all kosher about the mushroom talk on their web page, but i should i tell them i think they sent me contaminated syringes because i made some jars and they all turned out the same? i just dont want an email to fuck their system up ya know? never know whos watching.........


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Offlinelemon_lw
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353322 - 11/12/04 09:24 PM (12 years, 29 days ago)

try to leave out the whole cultivation part of it.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflinepsilocybinjunkieM
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353323 - 11/12/04 09:24 PM (12 years, 29 days ago)

dont mention your jars at all!
Tell them you recieved a contaminated syringe. They'll reply you can follow in kind after that. I wouldn't send them a message saying it fcked up all my jars. Tell them u recieved a contaminated syringe and your dissapointed.


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Offlinelemon_lw
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353324 - 11/12/04 09:24 PM (12 years, 29 days ago)

try to leave out the whole cultivation part out of it.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinesmoorhsmmm
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Re: help.. i got contam.. [Re: psilocybinjunkie]
    #3353327 - 11/12/04 09:26 PM (12 years, 29 days ago)

OK thats a big 10-4 good thing i asked! ty guys


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OfflinepsilocybinjunkieM
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Re: help.. i got contam.. [Re: smoorhsmmm]
    #3353336 - 11/12/04 09:29 PM (12 years, 29 days ago)

Glad to be of help, let us know how it works out for yah :wink:


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Offlinesmoorhsmmm
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Re: help.. i got contam.. [Re: psilocybinjunkie]
    #3374825 - 11/17/04 05:14 PM (12 years, 24 days ago)

wow all i can say is good job hawks eye, i just got an email from him, he's sending me new syringes for free USE THE HAWKS EYE! you cant go wrong


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