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OfflineMushroomFriend
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Adding water after inoculation
    #3352348 - 11/12/04 05:18 PM (12 years, 6 months ago)

I inoculized my pf cakes a week ago.
I have indications that there is not enough water in them. I used:
40 brf
40 water
140 VErm

I know this question has been aksed before but only 1 answer came.

Any people having xperience with adding water to pf jars afterwards? Id like to add 5-10CC of boiled water to each of them with a more or less sterile syringe. CAn syringes be PC-ed?

Tia! :laugh:


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Invisiblederx
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352361 - 11/12/04 05:21 PM (12 years, 6 months ago)

yes syringes can be PCed. you should be fine


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: derx]
    #3352379 - 11/12/04 05:24 PM (12 years, 6 months ago)

really derx, have you ever done this? adding water afterwards? (after a week in incubator wheroff the 1st 3 days below 80F)


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Invisiblederx
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352472 - 11/12/04 05:40 PM (12 years, 6 months ago)

i've never added water. I've read about people adding water and it was the procedure that you just stated.


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better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


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OfflineIGnosticAbhorI
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Re: Adding water after inoculation [Re: derx]
    #3352504 - 11/12/04 05:47 PM (12 years, 6 months ago)

Why not just do 2:1 for the water and not even have to worry about water? Mine has turned out Great(cake), After the first flush i had to dunk n roll but thats about as far as i go with "adding water", what you got going would worry me about contams, sure its sterile and such but i like to be protective with my lil shroomies :smile: and yea, 15 psi is what i believe they are suppose to be PCed at, gl.


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InvisibleATWAR
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352559 - 11/12/04 05:57 PM (12 years, 6 months ago)

If you intend on pressure cooking the syringes with water in them, good luck keeping it in there... My best advice is to make sure you have all traces of air out of them before you PC. Even then the chance of leakage is very high...

Your best bet would be to PC some water in a jar with a poly-fill lid. Once cooled you can insert the syringe through the poly-fill lid, and draw up the sterile water with minimal contamination risk. I am not sure how well adding water to the PF mix will go, but I have done this long ago using rye that was too dry. It worked but I had to add water gradually and shake it like all hell to get it distributed evenly and absorbed...


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Offlinelemon_lw
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Re: Adding water after inoculation [Re: ATWAR]
    #3352577 - 11/12/04 05:59 PM (12 years, 6 months ago)

ill tell you the way i added my "water". i have a karo solution and my jar was looking dry so i just shot in 10cc more karo water. its sterile and it can only help


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: ATWAR]
    #3352624 - 11/12/04 06:11 PM (12 years, 6 months ago)

Yes distribution is an issue but i just use the 4 holes and hope it will work a bit. Ill not put water in all of the jars, maybe in 4 of them or something.

I was planning to put the syringe in parts in the pc. So it will be drwoned in water. But then its gonna be hard to put it together without air coming in.

If I PC a waterfilled syringe with half a cc of air in it. Wouldnt the air in it be sterile too?

If problematic your "tek" will apply I guess!

Thanks! :thumbup:


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: derx]
    #3352630 - 11/12/04 06:12 PM (12 years, 6 months ago)

Ok thanx derx now must find a sterile way of doing things... :wink:


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: IGnosticAbhorI]
    #3352664 - 11/12/04 06:16 PM (12 years, 6 months ago)

Quote:

IGnosticAbhorI said:
Why not just do 2:1 for the water and not even have to worry about water? Mine has turned out Great(cake), After the first flush i had to dunk n roll but thats about as far as i go with "adding water", what you got going would worry me about contams, sure its sterile and such but i like to be protective with my lil shroomies :smile: and yea, 15 psi is what i believe they are suppose to be PCed at, gl.




I dont exactly understand it. You advice not to add water? 2:1? 2 what to 1 what? Contams why, the dryness, or you think adding water will contam the jars?
Sorry i dont get it!


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: lemon_lw]
    #3352679 - 11/12/04 06:20 PM (12 years, 6 months ago)

I havent reached the KARO stage yet :laugh: , what is it? What can it help in my situation?


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InvisibleATWAR
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352690 - 11/12/04 06:22 PM (12 years, 6 months ago)

Quote:

MushroomFriend said:
If I PC a waterfilled syringe with half a cc of air in it. Wouldnt the air in it be sterile too?





Yes, the air will be sterile, but that is not the problem. The problem lies with the air expanding when the syringes heat up, air expanding = less room for water = leaking water...


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Invisiblewoodzmun
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352695 - 11/12/04 06:24 PM (12 years, 6 months ago)

Well first of all why do you think there too dry? If this is your first grow it's best not to start second guessing yourself and then mucking with things. It's hard believe me I know it is, but if it ain't broke don't fix it. It's only been a week and your not going to be able to see moisture in a PF jar. The verm. absorbs it. You can only tell if there is too much water by it pooling in the bottom which you don't want. Anyways good luck MF.


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: ATWAR]
    #3352703 - 11/12/04 06:26 PM (12 years, 6 months ago)

wouldnt it take the easiest way out, through the needle?

Edit: when the PC is turned off the ater cools down a small implosion in the syringe, sucking a bit sterile water back in the syringe..

Makes sense?


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Edited by MushroomFriend (11/12/04 06:33 PM)


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: woodzmun]
    #3352716 - 11/12/04 06:30 PM (12 years, 6 months ago)

You have a point here. And why do I think its too dry hmmz. People here told me hehe! :laugh: By the looks too it looks so dry no moisture..

I guess you are right, I (Tha ubern00b) am not really sure, so I should wait fixing it. But when it is broken, I def will put a needle in their butties! :laugh:

BTW should a sporesyringe always have a colour like purplish, mine was rather clear I did not see spores, or cant they be spotted with the naked eye?


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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352727 - 11/12/04 06:32 PM (12 years, 6 months ago)

Huh?

Yes, that is what happens. I suppose if you placed the syringes with the needle up it might work, but I never tried that. I have had about 12 syringes end up half filled after a session with the PC. I don't do that anymore...


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Offlinelemon_lw
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352728 - 11/12/04 06:32 PM (12 years, 6 months ago)

using karo you can make 1cc into 100cc's. the mycelium grows inside the solution so it also speeds up your incubation time. in your situation you wouldnt have to worry about sterilizing water or what not cause your solution will be sterile and just shoot some in.


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: lemon_lw]
    #3352735 - 11/12/04 06:35 PM (12 years, 6 months ago)

Quote:

lemon_lw said:
using karo you can make 1cc into 100cc's. the mycelium grows inside the solution so it also speeds up your incubation time. in your situation you wouldnt have to worry about sterilizing water or what not cause your solution will be sterile and just shoot some in.




A kind of extra inoculation then? But not really a possibility since I havent any sporesyringe left, only a empty syringe.
But in the future its definately interesting stuff!


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OfflineMushroomFriend
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Re: Adding water after inoculation [Re: ATWAR]
    #3352745 - 11/12/04 06:36 PM (12 years, 6 months ago)

Quote:

ATWAR said:
Huh?

Yes, that is what happens. I suppose if you placed the syringes with the needle up it might work, but I never tried that. I have had about 12 syringes end up half filled after a session with the PC. I don't do that anymore...




Sorry I thought you meant with leakage that the syringe would be broken in a way but I understand you were just stating that.

BUt a syringe being drowned in water how can it get air in it?


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Invisiblewoodzmun
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Re: Adding water after inoculation [Re: MushroomFriend]
    #3352752 - 11/12/04 06:37 PM (12 years, 6 months ago)

I have had them both ways and they both worked fine. The darker one would just germinate faster and over a wider area, but clear ones still have thousands of spores there just microscopic. You should be fine. And I have PCed syringes before by putting them in quart jars needle up pointing to the sky and they did fine. But I would wait there MF, I thought that they looked dry too when I first started but they did fine. GL


Edited by woodzmun (11/12/04 06:39 PM)


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Microscope, Rye Grain, Vermiculite

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