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Offlineglennenp
Stranger

Registered: 11/08/04
Posts: 21
Last seen: 7 years, 10 months
Contamination
    #3349947 - 11/12/04 03:48 AM (12 years, 8 months ago)

How big do my precautions have to be in order to prevent against contamination? I am going to be able to use my school's laboratories to inoculate the agar as it shouldn't draw much attention but when I have to transfer the mycelium to the spawn I won't have that luxury. I'm worried, as my dorm room is anything but clean, that I'm going to contaminate my spawn during this step. This risk seems to be an equal threat when it comes time to case the spawn. What are some easy and effective ways to prevent against contaminants in these situations?


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Contamination [Re: glennenp]
    #3349971 - 11/12/04 04:04 AM (12 years, 8 months ago)

sounds like you need to clean your room for more than just this reason. :wink:

Sam


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OfflineRemi
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Registered: 10/04/04
Posts: 127
Last seen: 12 years, 7 months
Re: Contamination [Re: glennenp]
    #3349976 - 11/12/04 04:05 AM (12 years, 8 months ago)

Well I'd probably go into the bathroom or something of that sort. Clean your work area real good, either with alchohol or lysol whatever and do everything in there.
Building yourself a glovebox would cut down on the chances of contams alot aswell.


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Offlineglennenp
Stranger

Registered: 11/08/04
Posts: 21
Last seen: 7 years, 10 months
Re: Contamination [Re: Remi]
    #3349980 - 11/12/04 04:08 AM (12 years, 8 months ago)

Building a glovebox I could probably do, would I need fans, hepa filters, some type of makeshift antechamber and the whole bit or would the enclosed area be enough? I just don't know how great the risk for contamination is. I"m probably just overreacting.


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Offlinematticus
Stranger
Registered: 09/14/04
Posts: 13
Last seen: 12 years, 8 months
Re: Contamination [Re: Remi]
    #3349984 - 11/12/04 04:09 AM (12 years, 8 months ago)

Take all the precations necessary!! Seriously, anything you can do to keep everything sterile!


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OfflineRemi
Stranger

Registered: 10/04/04
Posts: 127
Last seen: 12 years, 7 months
Re: Contamination [Re: glennenp]
    #3349988 - 11/12/04 04:11 AM (12 years, 8 months ago)

You want to be as careful as possible.
Why take a chance? If your smart about it you will most probably get 100% success.
I'm sure there are a few teks on making gloveboxes around here.


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