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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Paul Stamets, Rye Grain

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Offlinebrianjamez
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Breaking up a popcorn substrate for casing Question...
    #3346087 - 11/11/04 12:12 PM (12 years, 30 days ago)

Whats the best (least chance of contams) way you all have found to break up a grain substrate when transfering from a jar to a casing?


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Offlineazurescens
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Re: Breaking up a popcorn substrate for casing Question... [Re: brianjamez]
    #3346341 - 11/11/04 01:36 PM (12 years, 30 days ago)

Shake up the jar 12-24 hours in advance before you transfer to allow the mycelium to recover a bit and then just transfer. Of course this should all be done in front of a flow hood. That is my friends technique and it has worked for years.


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Offlinecall_me_kido
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Re: Breaking up a popcorn substrate for casing Question... [Re: azurescens]
    #3346358 - 11/11/04 01:41 PM (12 years, 30 days ago)

Weird, I have some rye jars fully colonized and theres no way they are gonna be shaken. Maybe popcorn is much different, but theres no way i get my grains out without a sterilized fork.

Kido


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"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle


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OfflineZeroArmy27
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Re: Breaking up a popcorn substrate for casing Question... [Re: call_me_kido]
    #3346487 - 11/11/04 02:14 PM (12 years, 30 days ago)

Put a bag over the opening of the jar and spoon it out with a sterilized spoon (i dip mine in alcohol). Then break the colonized substrate up in the bag. I don't worry about my hands touching it (though i wear sterile latex gloves when i do this) or things settling on it from the air (HEPA filtered room makes me slightly more comfortable with this, though).

Works every time!

(sorry, edit because i put sterilized instead of colonized... woo, i need a nap.)


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"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

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Edited by ZeroArmy27 (11/11/04 02:15 PM)


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InvisibleKlingonFromUranus
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Re: Breaking up a popcorn substrate for casing Question... [Re: call_me_kido]
    #3346556 - 11/11/04 02:34 PM (12 years, 30 days ago)

Quote:

call_me_kido said:
Weird, I have some rye jars fully colonized and theres no way they are gonna be shaken. Maybe popcorn is much different, but theres no way i get my grains out without a sterilized fork.

Kido




Nope, popcorn is the same. There's no way you can break that sucker just by shaking. At least not once it's fully colonized.

Could you clarify this technique azurescens?


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Offlinecall_me_kido
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Re: Breaking up a popcorn substrate for casing Question... [Re: KlingonFromUranus]
    #3346568 - 11/11/04 02:39 PM (12 years, 30 days ago)

Thought so, Im prepared for enlightenment...I really hate having to traumatize my baby Myc with a sharp utensil...but it can be so damn uncooperative.


Kido


--------------------
"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle


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Offlineazurescens
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Re: Breaking up a popcorn substrate for casing Question... [Re: call_me_kido]
    #3346611 - 11/11/04 02:59 PM (12 years, 30 days ago)

When your popcorn/gain is fully colonized you pound it against your thigh very hard. It will break up. If you follow 'The Mushroom Cultivator', then you woud be banging it against a car tire that is mounted but my thigh works just fine. It's not like it will just fall apart after one or two shakes...you have to shake it hard.


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Offlineazurescens
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Re: Breaking up a popcorn substrate for casing Question... [Re: azurescens]
    #3346627 - 11/11/04 03:03 PM (12 years, 30 days ago)

By the way, this is assuming that you didn't fill your jars all the way to the top. there needs to be some space to shake.


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OfflineBaby_Hitler
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Re: Breaking up a popcorn substrate for casing Question... [Re: brianjamez]
    #3346657 - 11/11/04 03:12 PM (12 years, 30 days ago)

I bust up popcorn with a butter knife. I just stab down 3-4 inches and twist. Breaks it up in no time.


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OfflineChinaBoy
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Re: Breaking up a popcorn substrate for casing Question... [Re: Baby_Hitler]
    #3348035 - 11/11/04 07:39 PM (12 years, 30 days ago)

I use a big cool-aid spoon. Hey, hey, hey it's Cool-aid! I first mix cool-aid with the spoon, then sterilize it with rubbing alcohal. After I'm done making the case I drink the cool-aid in celebration.


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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Paul Stamets, Rye Grain

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