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Offlinedewey
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Registered: 05/26/04
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Light Grey Fuzz in Spore Needles?
    #3346020 - 11/11/04 11:58 AM (12 years, 10 months ago)

Does anyone know what this could be? I created a bunch of needles from a Keepers Creepers spore print that had bee kept in sterilized baggies, new needles, and distilled water were used. The initial needles produced many a shrooms the first round. Went back to use another needle that had been kept in the fridge and long and behold a fuzzy/whispy grey fluff is in all of the needles. There are none in my Blue Meanie syringes? Any ideas?

dewey


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OfflineKaptKid
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3346123 - 11/11/04 12:25 PM (12 years, 10 months ago)

Shot up 1 jar to see if it contams. If so, you know the story.GL


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InvisibleKyKid
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Registered: 10/22/04
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Re: Light Grey Fuzz in Spore Needles? [Re: KaptKid]
    #3346257 - 11/11/04 01:02 PM (12 years, 10 months ago)

it could be germination in the needle but i doubt it if its grey, are you sure its not white, place it in front of a light and see if it white if so itll jump start you colonization time and nothing wrong with that but yeah i was kapt try one jar or make up a liquid culture or do some agar to see if its contams or not. good luck with it.


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Offlinedewey
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3346440 - 11/11/04 02:05 PM (12 years, 10 months ago)

It's actually greyish/white and in a culture as we speak. How will i know if the culture becomes contaminated?

dewey


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InvisibleKyKid
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3346480 - 11/11/04 02:12 PM (12 years, 10 months ago)

Itll smell funny along with fast growth of contams, contams take over quite quickly and before you know it the culture will be all one wired color or smell funky. just keep an eye out, and if the culture grows and you still cant tell just knock up on jar or some agar


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Offline4hodmt
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Re: Light Grey Fuzz in Spore Needles? [Re: KyKid]
    #3346543 - 11/11/04 02:29 PM (12 years, 10 months ago)

the spores have germinated. itll just colonize faster. enjoy.

GOOD LUCK!


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Offlinedewey
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Re: Light Grey Fuzz in Spore Needles? [Re: 4hodmt]
    #3352074 - 11/12/04 04:26 PM (12 years, 10 months ago)

Thanks Everyone ... is spore germination in water a common thing or is it species dependent?


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InvisiblePrisoner#1
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3352115 - 11/12/04 04:36 PM (12 years, 10 months ago)

with fresh spores its pretty common.


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Offlinedewey
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3352119 - 11/12/04 04:36 PM (12 years, 10 months ago)

So to reitertae the grey floating mass is germinated spores and i should not have to worry about it? What temp should the karotek jar be kept at?

dewey


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InvisiblePrisoner#1
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3352213 - 11/12/04 04:55 PM (12 years, 10 months ago)

incubate it at 82-86F just as you would the PF jars or grains.


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Offlinedewey
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Re: Light Grey Fuzz in Spore Needles? [Re: Prisoner#1]
    #3352355 - 11/12/04 05:19 PM (12 years, 10 months ago)

After i have inoculated my jars ... can i keep the remaining solution in the fridge? How long can i keep in there for?

dewey


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InvisiblePrisoner#1
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Re: Light Grey Fuzz in Spore Needles? [Re: dewey]
    #3352503 - 11/12/04 05:47 PM (12 years, 10 months ago)

it's usualy good for 3-4 months as long as it remains sterile,
some people have reported more than 6 months


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