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Offlinejonas
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spores viable after nearly two years?
    #3331455 - 11/07/04 11:33 PM (12 years, 1 month ago)

I have several unopened spore syringes from a year and a half ago and I wanted to use them, will they still be viable? They have been kept in the dark in temps of between 65-70.


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OfflineRRhoads
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Re: spores viable after nearly two years? [Re: jonas]
    #3331475 - 11/07/04 11:39 PM (12 years, 1 month ago)

yes


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Offlinejonas
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Re: spores viable after nearly two years? [Re: RRhoads]
    #3331479 - 11/07/04 11:42 PM (12 years, 1 month ago)

Okay, I was kind of concerned because about 6 months ago I tried using one of them to inoculate some popcorn jars but I know I didnt prepare them right, it was a rushed job, so they didnt have the right moisture content and they never started colonizing, I think it was a combination of improper preparation, sterilization, and incubation conditions. It was such a half assed job because I had to keep it well hidden from a room mate and I had to do all the preparation while my room mate was making trips to walmart and such. Now I got the place to myself so I can do whatever I please ... so its time to get back into growing mushrooms.


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InvisibleKoala Koolio
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Re: spores viable after nearly two years? [Re: jonas]
    #3331656 - 11/08/04 12:43 AM (12 years, 1 month ago)

There will surely be less viable spores than originally. But it should be enough if it was a good syringe to begin with.


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Offlinejonas
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Re: spores viable after nearly two years? [Re: Koala Koolio]
    #3331660 - 11/08/04 12:45 AM (12 years, 1 month ago)

Got em from Ralph. His are usually pretty good, right?


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InvisibleKoala Koolio
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Re: spores viable after nearly two years? [Re: jonas]
    #3331781 - 11/08/04 01:23 AM (12 years, 1 month ago)

Yep.


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Offlinedmc_
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Re: spores viable after nearly two years? [Re: Koala Koolio]
    #3331952 - 11/08/04 02:48 AM (12 years, 1 month ago)

Expect a couple extra days for germination.


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Offlinediscman1
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Re: spores viable after nearly two years? [Re: dmc_]
    #3332104 - 11/08/04 04:24 AM (12 years, 1 month ago)

I had a syringe stored for 7-8 months, originally got it from TheSporeWorks.

It was stored in a dark place, but.. the time period spanned through summer, where it was in the 90s - low 100s for several weeks.

When I finally got around to trying my hand at this hobby, the syringe was no longer viable.

The first time I knocked up 12 jars, none of them grew at all. The 2nd time I injected 5cc into a pint jar, still nothing. The 3rd time I shot the remaining 3cc spore solution into the sink and reclaimed the syringe for other uses. :P


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Offlinehyphae
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Re: spores viable after nearly two years? [Re: discman1]
    #3332366 - 11/08/04 07:51 AM (12 years, 1 month ago)

I've stored them in the fridge for over 6yrs. and they were viable. Also don't forget to shake the hell out of that syringe in order to evenly distribute the spores as they will clump together. GL


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