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Invisibleikoiko79
Stranger
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Registered: 11/02/04
Posts: 59
how long can a PC jar w/ BRF stay sterile for?
    #3330006 - 11/07/04 09:59 AM (19 years, 4 months ago)

It has been foil wrapped on top and stored in a cool dark place. How long can I keep it before its worthless?

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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: how long can a PC jar w/ BRF stay sterile for? [Re: ikoiko79]
    #3330015 - 11/07/04 10:04 AM (19 years, 4 months ago)

Theoretically it could stay sterile until the sun goes Supernova and we're all turned into crispy critters. :wink:

If it's still sterile, it should be fine to use.

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Offlinecall_me_kido
Philosopher
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Registered: 10/26/04
Posts: 354
Loc: In your dreams
Last seen: 14 years, 3 months
Re: how long can a PC jar w/ BRF stay sterile for? [Re: discman1]
    #3330094 - 11/07/04 10:45 AM (19 years, 4 months ago)

i agree with discman, as long as there isnt a viable exposure to the contents 0 spores = 0 infections. If theres nothing in the jar to colonize then nothing will colonize.

Kido


--------------------
"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle

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Invisibleikoiko79
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Registered: 11/02/04
Posts: 59
Re: how long can a PC jar w/ BRF stay sterile for? [Re: call_me_kido]
    #3330413 - 11/07/04 04:37 PM (19 years, 4 months ago)

cool thnaks for the info

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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: how long can a PC jar w/ BRF stay sterile for? [Re: ikoiko79]
    #3330421 - 11/07/04 04:39 PM (19 years, 4 months ago)

I would like to add that, if the jar has been sitting for like more than a month, I would begin to question whether the water content has changed or not...

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OfflineTripster
200+ trips wise

Registered: 10/10/04
Posts: 275
Last seen: 17 years, 6 months
Re: how long can a PC jar w/ BRF stay sterile for? [Re: discman1]
    #3330464 - 11/07/04 04:50 PM (19 years, 4 months ago)

For example, I have a jar that failed to grow after inoculation, for whatever reason I'm not sure. It's been over 3 weeks, close to 4, and not only is there no growth of myc, there's no growth at all. I autoclaved the jars and I still think that jar is sterile. I won't know for sure until I open it though, the smell will tell for sure.


--------------------
You've raped! I feel dirty
It hurt! As a child
Tied down! That's a good boy
And fucked! Your own child
I scream! No one hears me
It hurt! I'm not a liar
My God! Saw you watching
Mommy why?! Your own child -Korn

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