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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Wild Bird Seed

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Offlinephantasm
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Registered: 09/10/04
Posts: 801
Last seen: 9 years, 9 months
G2G RULES!!!!
    #3327285 - 11/06/04 12:37 PM (12 years, 8 months ago)

well 2 days ago i did g2g with one of my pint brazillian jars i took a quater of the 100% and put it in a jar of WBS i did this for 3 other jars so basicly 1/4 colonized in every jar so i have 4 jars at 25% to start with. i looked at tehm today and wham they are allready like 50-60% done so like 25% more than they were two days ago that so fast i love it i was looking to do it to waste time until my terrarium is empty but it looks like they will still finish way ahead of everything in my terrarium.


-mike

thanks to everyone who helped me on this subject

also 0 contamns in every jar

i think next time i will do many more jars this way probably about 10 from 1 jar but this was more or less a practice run and all went well


--------------------
Only God Grows Amanita Muscaria


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Invisibleohmatic
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: G2G RULES!!!! [Re: phantasm]
    #3327312 - 11/06/04 12:50 PM (12 years, 8 months ago)

how did u do the g2g transfer?

openair/glovebox/flowhood, and how did u do it?
like spraw the air with lysol or alike,
wipe the jars down and stuff?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlineintrigue
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Re: G2G RULES!!!! [Re: ohmatic]
    #3327507 - 11/06/04 02:03 PM (12 years, 8 months ago)

glove box or flow hood is the best way. i don't think u could do it in open air. maybe you could using the oven tek


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Offlinescatmanrav
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Registered: 05/08/04
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Re: G2G RULES!!!! [Re: intrigue]
    #3327632 - 11/06/04 02:43 PM (12 years, 8 months ago)

You sure can do it open air if you make the air sterile enough. I've done probably a hundred quarts grain to grain, all open air. Probably a 2-5% contam rate. I did work in my bathroom with all rugs and towels and EVERYTHING (tooth brushes, cups sitting out, ect) from the bathroom and ran 2 HEPAs in for 2 hours spraying Oust around the bathroom when the HEPAs go in (2 horus before working) and then 10 minutes before I go in, then as I go in. I take a shower 2 hours before (just before leaving the HEPAs in and put on clean boxers only..then go in with only boxers, gloves and a paint mask on. Use alcohol for the spoon and be quick about it. This is where I do innculations, casings, G2G, liquid cultures, prints, syringes, agar work, everything. It works as a giant glove box.

You could have used a pint jar to do a dozen more pints..at least. I used a quart jar to do 2 dozen quarts before and I could have used even less and done a few more jars..more like 30-40 probably. You only need one little tiny peice the size of a nickel, if that.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlineintrigue
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Re: G2G RULES!!!! [Re: scatmanrav]
    #3328902 - 11/06/04 11:38 PM (12 years, 8 months ago)

wow that's nice i cant do anything in the open air a simple innoc using a spore syringe done in open air failed 100 percent.


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Offlinephantasm
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Registered: 09/10/04
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Re: G2G RULES!!!! [Re: intrigue]
    #3328909 - 11/06/04 11:42 PM (12 years, 8 months ago)

yeah i used a sterilized glove box

i know i could have done way more but i have a room issue in my incubator as of right now until my 2 new casings come out so no need for more than i did plus if i want more i can jsut do it again later

i cleaned it with a warm/hot bleach solution then
i sprayed the inside with lysol and wiped it down with a lysol wipe
i then sprayed all the air around it too allowed it to evaporate
with lid loosely on

yeah im a little over protective of my little buddies

-mike


--------------------
Only God Grows Amanita Muscaria


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OfflineKaptKid
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Re: G2G RULES!!!! [Re: phantasm]
    #3328955 - 11/07/04 12:03 AM (12 years, 8 months ago)

Sunday I'm trying my 1st large bulk 18in x 24in x 6in high.Thats 5 qt's straw,3qt's poo and 6qt's wbs. Have cleaned and will clean more.
1st time out of the box.I hope things go alright.


Peace


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Offlineintrigue
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Re: G2G RULES!!!! [Re: KaptKid]
    #3328957 - 11/07/04 12:03 AM (12 years, 8 months ago)

best of luck to u


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Offlinescatmanrav
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Re: G2G RULES!!!! [Re: intrigue]
    #3329444 - 11/07/04 03:12 AM (12 years, 8 months ago)

Quote:

intrigue said:
wow that's nice i cant do anything in the open air a simple innoc using a spore syringe done in open air failed 100 percent.




If you take all the procautions I mentioned you wont. Also aways make sure you wipe down all injection points with alcohol.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineintrigue
MMMM hard trance
 User Gallery
Registered: 09/06/04
Posts: 248
Last seen: 5 years, 2 months
Re: G2G RULES!!!! [Re: scatmanrav]
    #3329838 - 11/07/04 10:25 AM (12 years, 8 months ago)

Quote:

scatmanrav said:
Quote:

intrigue said:
wow that's nice i cant do anything in the open air a simple innoc using a spore syringe done in open air failed 100 percent.




If you take all the procautions I mentioned you wont. Also aways make sure you wipe down all injection points with alcohol.




yay i tired doing all of that except the 2 hepa filters. the big problem is i live in an old apartment building. every room has carpet even the bathroom. i bleached bombed the room, then Lysol waited till everything settled. i used latex gloves a mask and never let the needle touch anything but a napkin soaked in bleach.


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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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