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Amazon Shop: ½ Pint Jars, Lab Glassware

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Offlinepebbles2004
sponge

Registered: 10/11/04
Posts: 108
Last seen: 4 years, 7 months
newb help
    #3323640 - 11/05/04 10:19 AM (12 years, 8 months ago)

i have nine pf jars inoculated with about 8 cc's apiece of liquid myc, they have been sitting in a TiT for 2 days now at around 82-84F and there are no signs of any growth . is this normal or should something have shown up already?


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InvisibleLifenergy
Yo Mama

Registered: 08/05/04
Posts: 766
Re: newb help [Re: pebbles2004]
    #3323647 - 11/05/04 10:23 AM (12 years, 8 months ago)

8 cc's a piece? If you used that much spore solution in each jar, you surely threw off the water content of the jars, if they are standard 1/2 pint jars. You might have to toss them and start over. 1cc per jar is more than enough. Still, 2 days, even with myc culture, is a little early to be worrying. Depending on your parameters, it might take a few more days to see growth.


--------------------
Everything that we see is a shadow cast by that which we do not see.


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Offlinefloydguitar
smooth guitarist

Registered: 10/14/04
Posts: 187
Last seen: 11 years, 2 months
Re: newb help [Re: Lifenergy]
    #3323664 - 11/05/04 10:29 AM (12 years, 8 months ago)

wait a couple more days. i have waited as long as 5 days before i have seen any growth. 8cc's a jar is way to much. lifenergy is right about the water content. also it is a waste. if you used 8cc's a jar on nine jars that is 72cc's that you used. with that much you could of had 48 jars on the low end and 72 on the high end. oh well next time you know, good luck.


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OfflineBrady
randomizer
Registered: 09/17/04
Posts: 170
Loc: on a damn island , FL
Last seen: 6 years, 6 months
Re: newb help [Re: pebbles2004]
    #3323711 - 11/05/04 10:47 AM (12 years, 8 months ago)

if you want to do big injections , you need to reduce the water content when making your jars. This loses its effectiveness past 5cc. So like If you normally make your jars with 60cc of water, make it with 56cc, once its pc'ed and ready to go inject divide 5cc into the four holes(5cc total). Make sure you flip your jars every coupld days to help distribute the liquid. more myc, more growth sooner, but past 5cc , not much point.


--------------------
I love to trade , trade with me!


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Offlinepebbles2004
sponge

Registered: 10/11/04
Posts: 108
Last seen: 4 years, 7 months
Re: newb help [Re: Brady]
    #3323725 - 11/05/04 10:52 AM (12 years, 8 months ago)

what do you guys use to accurately measure out your water?


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 11 years, 3 months
Re: newb help [Re: pebbles2004]
    #3323750 - 11/05/04 10:59 AM (12 years, 8 months ago)

Graduated cylinder.



--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 12 years, 7 months
Re: newb help [Re: Silven]
    #3323756 - 11/05/04 11:00 AM (12 years, 8 months ago)

lol.....

I use a measuring cup.

But if I was doing delicate work like that I would also use a graduated cylinder.


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Amazon Shop: ½ Pint Jars, Lab Glassware

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