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OfflineAnonymousNewbie
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Registered: 06/20/04
Posts: 211
Loc: England
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Mushroom Spores under microscope?
    #3321338 - 11/04/04 08:48 PM (13 years, 1 month ago)

Say you get some spore syringes, and you inject the spore solution onto your microscope slides - how can you tell if the spores would actually develop myc. or if they are simply "dead"? What would they look like? any links to pictures?


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InvisibleJoshua
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Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Mushroom Spores under microscope? [Re: AnonymousNewbie]
    #3327043 - 11/06/04 10:34 AM (13 years, 1 month ago)

There is no way I am aware of to tell this other than live culturing. A dead spore looks the same as a live spore in most cases. One might look for abnormalities in the spore structure as an indication of possible poor germination.

I am aware that spores when suspended in water for prolonged period would begin the germination process. These spores would be present with a protruding hyphae, this would give a greater degree of certainty that the spores are viable.

Joshua


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Invisiblebaltazar
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Registered: 02/23/04
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Re: Mushroom Spores under microscope? [Re: Joshua]
    #3341413 - 11/10/04 02:10 PM (13 years, 1 month ago)


I made some spore pictures long time ago, sorry for the realy bad quality pics. It was taken with a mobile phone camera through a home microscope on 600x magnification ...



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InvisibleOdin 13
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Registered: 01/08/00
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Re: Mushroom Spores under microscope? [Re: baltazar]
    #3358072 - 11/14/04 01:42 AM (13 years, 1 month ago)

The live ones will be carrying signs which say "grow me!" The dead ones will all be in coffins and their buddies will be carrying them to the graveyard. Clear enuff twit? And this is in advanced none the less?


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Offlineshirley knott
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Re: Mushroom Spores under microscope? [Re: Odin 13]
    #3359387 - 11/14/04 12:18 PM (13 years, 1 month ago)

Quote:

Odin 13 said:
The live ones will be carrying signs which say "grow me!" The dead ones will all be in coffins and their buddies will be carrying them to the graveyard.



:rotfl:


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OfflineDestr0yingAngel
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Registered: 11/08/04
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Loc: Iowa, USA
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Re: Mushroom Spores under microscope? [Re: AnonymousNewbie]
    #3367643 - 11/16/04 07:17 AM (13 years, 1 month ago)

Is it just me, or isn't there a really simple way to figure this problem out?


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OfflineAnnoA
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Re: Mushroom Spores under microscope? [Re: Destr0yingAngel]
    #3367693 - 11/16/04 08:10 AM (13 years, 1 month ago)

There is. It does not involve microscope though.


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Invisibleohmatic
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Re: Mushroom Spores under microscope? [Re: Anno]
    #3367891 - 11/16/04 10:06 AM (13 years, 1 month ago)

id suggest shooting up some karo and ull be able to see if ur sporeload is vital or not.
peace ohm :mushroom2:


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OfflineAnnoA
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Re: Mushroom Spores under microscope? [Re: ohmatic]
    #3367941 - 11/16/04 10:25 AM (13 years, 1 month ago)

It would be better he inoculated agar or a pf cake. If you inoculate a liquid medium you won't be able to tell immediately whether the growth is a contaminant or the mycelium.


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Invisibleohmatic
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Re: Mushroom Spores under microscope? [Re: Anno]
    #3368030 - 11/16/04 10:58 AM (13 years, 1 month ago)

Quote:

Anno said:
If you inoculate a liquid medium you won't be able to tell immediately  whether the growth is a contaminant or the mycelium.




very true.

if ur aim is 'instant' (well it will take a couple of days anyway) check for contamination,
i would go for anno's advice of inoculating agar.
it should be the fastest way to check for possible contamination.

if u'd still like to try inoculating liquid media id suggest u to mix a teaspoon of honey with about 100ml of water,
pressure cook it and inoculate, OR you could follow this tek: http://www.shroomery.org/index/par/7996
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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