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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain

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Offlinebigjsheets
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Registered: 11/02/04
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How long to sterilize prior to inoculate
    #3311403 - 11/02/04 10:32 PM (12 years, 3 months ago)

Just wondering.... If i sterilize quart jars of rye in a pressure cooker (tyvek under lids), how long can they sit before innoculating them? In general i have found the tyvek method keeps jars well sealed but just wondering what you guys think.


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Offlinejent
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Re: How long to sterilize prior to inoculate [Re: bigjsheets]
    #3314148 - 11/03/04 12:38 PM (12 years, 3 months ago)

i always wait over night, last thing you want to do is rush it by 12 hours just to kill all your spores. Not worth it, be extra safe. Just because the outside feels cool dosent mean the inside is.


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the jent


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InvisibleMykro_Guy
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Re: How long to sterilize prior to inoculate [Re: bigjsheets]
    #3315009 - 11/03/04 03:08 PM (12 years, 3 months ago)

i usually wait around 4 hours or so...its winter where i am so i just take them out of the PC and load another btach of jars by the time all my jars are done my first batch it really cold. wait around 4 hours and thats safe... i like to inoculate the same day cuz i dont have time to mess with jars 2 days in a row cuz of school and work. if there cold to the touch add another hour to that and your fine... the spore solution runs along the glass anyways.


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Offlinebigjsheets
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Re: How long to sterilize prior to inoculate [Re: Mykro_Guy]
    #3328610 - 11/06/04 09:53 PM (12 years, 3 months ago)

I really wanted to know how long they can sit. Is 2-3 days too long before inoculation?


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OfflineKaptKid
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Re: How long to sterilize prior to inoculate [Re: bigjsheets]
    #3328639 - 11/06/04 10:02 PM (12 years, 3 months ago)

Quote:

bigjsheets said:
I really wanted to know how long they can sit. Is 2-3 days too long before inoculation?




No thats not to long.Done it a few times.  GL :mushroom2:


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Child of the 60's, Tripping ever since.


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Offlinecall_me_kido
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Re: How long to sterilize prior to inoculate [Re: KaptKid]
    #3329736 - 11/07/04 08:59 AM (12 years, 3 months ago)

Assuming all contams were destroyed on Pcing, Id say it will be fine to let them sit. Germination from inoculation takes 3-5 days on rye anyway, mycelium isnt contam resistant until weeks of incubation, during that time it is leaving exposed substrate anyway. You could likely leave properly sterlized jars for much longer if necessary, though not recommended, especially in the conditions warranting foreign growth, (Good temps and light exposure) both of which encourage bacteria and mold colonization.

Kido


--------------------
"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle


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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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