Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Petri Dish

Jump to first unread post. Pages: 1
Invisibledownforpot
Stranger
Male
Registered: 06/25/01
Posts: 5,715
testing syringe for contamination
    #3310970 - 11/02/04 08:37 PM (13 years, 6 months ago)

I have a syringe full of liquid mycelium from honey water. I have a few sterile disposable petri dishes and I was wondering how I could test it for contaminant. Should I use honey again? If so, how should I prepare it(I used a PC to sterilize the HW last time). Anything else I could use that is easy to get and use?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: testing syringe for contamination [Re: downforpot]
    #3313901 - 11/03/04 12:06 PM (13 years, 6 months ago)

Make an agar plate, squirt a minute amount of solution onto the plate and spread the solution across the plate.

Within 1 week you will know if you have a sterile solution.

Another option is to prepare a substrate and inoculate onto the substrate. I usually do a test jar of grain before inoculating a whole batch of jars.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: testing syringe for contamination [Re: Joshua]
    #3314394 - 11/03/04 01:24 PM (13 years, 6 months ago)

Quote:

Joshua said:
I usually do a test jar of grain before inoculating a whole batch of jars.




as straightforward as this is i never ever considered doing that (stupid  me)
and recently justlost 5 jars due to a fucked syringe...
darn, will do that in future.

btw, id suggest the same thing with the agar plate, to see if it is ok.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinexistenz
Stranger

Registered: 08/05/04
Posts: 159
Last seen: 12 years, 8 months
Re: testing syringe for contamination [Re: ohmatic]
    #3316432 - 11/03/04 08:57 PM (13 years, 6 months ago)

wouldn't contams grow in the honey jar if it were contam'd? or do you mean that the syringe got contam'd in the process?

yeah, what joshua said.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: testing syringe for contamination [Re: xistenz]
    #3318770 - 11/04/04 09:24 AM (13 years, 6 months ago)

A liquid culture is hard to visually screen for contamination. A lot of fungi look the same in liquid culture.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Karo as a test for contaminated syringe? vermonster 504 3 03/19/04 06:47 AM
by Joshua
* mycelium or contamination? henryp 1,851 5 01/08/02 02:57 PM
by henryp
* Syringe Contamination TrippingBillies 731 2 05/22/02 10:50 AM
by PGF
* got some contamd spores what use do thay have? MAGICSNOOP 374 2 05/22/03 05:31 PM
by PDU
* Does this look like contamination to you? paranoid2 926 9 06/17/02 11:14 PM
by durban_poison
* I am scared to do a Print/syring! fearforce7 4,975 18 02/24/17 09:11 PM
by mushpunx
* contamd spore syringe? professorFATTYCAP 479 6 04/24/15 09:11 PM
by cronicr
* Empty Contamd Jars.. PC 15psi and Re-Use Them? OregonMushys 294 2 07/29/14 04:56 PM
by SpitballJedi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
527 topic views. 20 members, 81 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Kratom Eye
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.005 seconds on 19 queries.