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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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Invisibledownforpot
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testing syringe for contamination
    #3310970 - 11/02/04 08:37 PM (12 years, 2 months ago)

I have a syringe full of liquid mycelium from honey water. I have a few sterile disposable petri dishes and I was wondering how I could test it for contaminant. Should I use honey again? If so, how should I prepare it(I used a PC to sterilize the HW last time). Anything else I could use that is easy to get and use?


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InvisibleJoshua
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Registered: 10/27/98
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Re: testing syringe for contamination [Re: downforpot]
    #3313901 - 11/03/04 12:06 PM (12 years, 2 months ago)

Make an agar plate, squirt a minute amount of solution onto the plate and spread the solution across the plate.

Within 1 week you will know if you have a sterile solution.

Another option is to prepare a substrate and inoculate onto the substrate. I usually do a test jar of grain before inoculating a whole batch of jars.

Joshua


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Invisibleohmatic
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Re: testing syringe for contamination [Re: Joshua]
    #3314394 - 11/03/04 01:24 PM (12 years, 2 months ago)

Quote:

Joshua said:
I usually do a test jar of grain before inoculating a whole batch of jars.




as straightforward as this is i never ever considered doing that (stupid  me)
and recently justlost 5 jars due to a fucked syringe...
darn, will do that in future.

btw, id suggest the same thing with the agar plate, to see if it is ok.
peace ohm :mushroom2:


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Offlinexistenz
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Registered: 08/05/04
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Re: testing syringe for contamination [Re: ohmatic]
    #3316432 - 11/03/04 08:57 PM (12 years, 2 months ago)

wouldn't contams grow in the honey jar if it were contam'd? or do you mean that the syringe got contam'd in the process?

yeah, what joshua said.


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InvisibleJoshua
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Re: testing syringe for contamination [Re: xistenz]
    #3318770 - 11/04/04 09:24 AM (12 years, 2 months ago)

A liquid culture is hard to visually screen for contamination. A lot of fungi look the same in liquid culture.

Joshua


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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