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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
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Is there somthing wrong?
    #3305983 - 11/01/04 07:48 PM (12 years, 1 month ago)

Well i inoculated my pf tek on 10/05/04 of the b+ race with 6oz jars and they still arent fully colonized.they have about 20% more to fully colonize,does anyone know why this is taking so long? I have the temp at about 85 and in dark place. I got two contams out of 8 jars so far.I used 105ml of vermaculit and 30ml of brf, the water was put in just so that it was moist.I also did not pack it in the jars.I have plastic lids on the jars with tin foil on them.About two days ago i put the jars upside down but not much change in growth speed..........so what gives? :crying:


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InvisiblePrisoner#1M
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Re: Is there somthing wrong? [Re: cougercruiser]
    #3305998 - 11/01/04 07:51 PM (12 years, 1 month ago)

patience, your temps may be a little low or it may be some other variable
some times they just decide to stall for a bit... give it more time


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InvisibleSam1912
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Registered: 09/23/04
Posts: 2,142
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Re: Is there somthing wrong? [Re: Prisoner#1]
    #3306019 - 11/01/04 07:57 PM (12 years, 1 month ago)

If the jars did stall, it takes a bit of time to get them moving again..  think about driving a car.  It's also called momentum for myc growth. :wink:

Sam


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
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Re: Is there somthing wrong? [Re: Sam1912]
    #3306186 - 11/01/04 08:36 PM (12 years, 1 month ago)

but why did they stall,can i takem to a machanic to see if there is somthing wrong>? :grin:


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InvisibleSam1912
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Registered: 09/23/04
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Re: Is there somthing wrong? [Re: cougercruiser]
    #3306249 - 11/01/04 08:51 PM (12 years, 1 month ago)

too much moisture on surface(this is number one reason for stalled jars), too little(Very unlikely), too much myc piss sitting on uncolonized substrate, cold temperature, hot temperature, packed jars, contam, way too high CO2(yes, high CO2 is good, but too high CO2 will eventually slow the growth), etc..

There's a lot of reasons... You just gotta experiment and see. Always experiment with jars you plan to experiment with... If you take a jar and say inject more water, the results will be completely useless for the future grow. Not to mention increased chances of contam...

Good luck

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Invisiblemadsickpenguin
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Re: Is there somthing wrong? [Re: Sam1912]
    #3306341 - 11/01/04 09:18 PM (12 years, 1 month ago)

Sam, I noticed you said "packed jars"...does this suggest to not use the tamp down tek that the PF tek calls for?


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Offlinedmtrypr
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Registered: 07/15/04
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Re: Is there somthing wrong? [Re: madsickpenguin]
    #3306391 - 11/01/04 09:32 PM (12 years, 1 month ago)

I actually stopped using pf tek because of the unreliability of the method. Sometimes my jars would colonize very quickly, sometimes slower than my patience would endure. I noticed that spore syringes really vary wildly, and maybe you got a more dilute syringe. I have also had stalling with low moisture jars moreso than those that erred on the wet side. I have recently started using wild bird seed (kash n karry variety, ~$3.00) and it has worked soooo much better I cannot believe I even bothered with brf cakes. The birdseed only needs to be soaked overnight to be ready for sterilization. I even tried boiling the jars(2 hrs, w/ lid on) instead of p.c. and it worked fine too. I can go from 0% to 100% colonization in 4-5 days max. Then, you can just use grain to grain transfers to create an infinite supply of spawn, something that is a royal pain in the ass with regular cakes.


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"There is no greater power in heaven and earth than the thought of the son of man. Though unseen by the eyes of the body,yet each thought has mighty strength, even such strength can shake the heavens." -Gospel of the Essenes


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InvisibleSam1912
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Re: Is there somthing wrong? [Re: madsickpenguin]
    #3306416 - 11/01/04 09:38 PM (12 years, 1 month ago)

I think packing(even the slightest) makes huge difference in time.  I honestly think this is one of the most common misunderstanding with newbies.  I really don't think they understand what not packing mean.

I'd advise not to pack.  Which means really really really fluff that mixture before you load it ever so gently into the jars.  That's why I don't really like the phrase lightly pack.  I just tap(very very gently) the jars on the counter as I load the jars.  It looks slightly unstable fluffy before you put the dry verm on.

I had a bit of trouble with my very first set of jars(took almost twice as long as my GTs).  I just didn't know what fluffy really meant...

I don't know if this is true and I'll really have to come up with a way to test it out, but the BU jars I had problem with during colonization aren't fruiting very well, either(compared to other BUs that I made later).  I'm inclined to think that you have to ride out the momentum and switch to generative growth using that momentun to get most out of the cakes.  I'm would probably put 10% faith on this without further experiments specifically designed to test this...

In any case...  try not packing.  I'm sure you can determine which works best for you. :wink:

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Offlinehyphae
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Re: Is there somthing wrong? [Re: madsickpenguin]
    #3306445 - 11/01/04 09:44 PM (12 years, 1 month ago)

A slight tamp is fine IME. There will not be too much co2 in a jar that has good gas exchange. When the gas is not allowed to escape it builds up pressure I believe it's more of this co2 pressure than the PPM concentration that causes the excess myc piss.


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Invisiblemadsickpenguin
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Re: Is there somthing wrong? [Re: dmtrypr]
    #3306462 - 11/01/04 09:48 PM (12 years, 1 month ago)

dmtrypr...whoa whoa whoa, 4-5 days max?!  What tek are you using, specifically..and what alterations have you made to tek?  4-5 days is a VERY short time and I, along with many others I'm sure, would like to know your methods  :mushroom2: :mushroom2: :mushroom2:


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Invisiblegeorge castanzaM
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Re: Is there somthing wrong? [Re: dmtrypr]
    #3307963 - 11/02/04 06:24 AM (12 years, 1 month ago)

(4-5days)is that msi or a g2g?


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





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Offlinedoc34
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Re: Is there somthing wrong? [Re: george castanza]
    #3307973 - 11/02/04 06:35 AM (12 years, 1 month ago)

Quote:

I can go from 0% to 100% colonization in 4-5 days max.




What size jars are you using?-->1/2 pints?

My pints colonize in about 7-8 days from liquid mycelium(karo tek)and my quarts take about 10-12 days.
You can add 3-7 days to that time if you are using multispore(germination of spores)
I have never had a pint or quart colonise in 4-5 days!

Would you care to elaborate more on your technique?


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Offline2Cents
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Re: Is there somthing wrong? [Re: cougercruiser]
    #9385877 - 12/07/08 05:45 PM (8 years, 1 day ago)

too hot dude


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OfflineCascadian
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Re: Is there somthing wrong? [Re: 2Cents]
    #9386093 - 12/07/08 06:15 PM (8 years, 1 day ago)

Why are you giving advice to someone who made their jars on
Quote:

10/05/04




This thread died
Quote:

11/02/04 03:35 AM (4 years, 1 month ago)




:dumblol:


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Offlineveda_sticks
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Re: Is there somthing wrong? [Re: Cascadian]
    #9386566 - 12/07/08 07:34 PM (8 years, 1 day ago)

I thought that the trusted cultivators would have spotted that hes incubating at 85F which is too hot. That could certainly slow growth and cause metobolites.

Also, higher temperatures can stimulate  moulds and bacteria. colinise at room temp 75F.

Overly wet substrate, improper gas exchange, using jars that are taller than they are wide are among the reasons for slow/stalled growth.


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Franks Simple Coir/Verm Tek
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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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