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 kjb1891
MycoManiac
Registered: 07/17/02
Posts: 341
Last seen: 12 years, 7 months
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 Liquid Culture Tips 
#3305705 - 11/01/04 04:23 PM (19 years, 5 months ago) |
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What tips or tricks has everyone found to work well for them in their experiences making liquid mycelium cultures(either karo or honey)?
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MoneyAddyct
Picaro
Registered: 09/10/04
Posts: 410
Last seen: 13 years, 10 months
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Re: Liquid Culture Tips [Re: kjb1891]
#3305726 - 11/01/04 04:32 PM (19 years, 5 months ago) |
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I don't have any tips; but the method I used from scatmanrav works great. Use a 1/2 pint jar. Poke 1 hole in the center and place a piece of tyvek over it. Fill a 1/2 pint jar with 100ml of water and 1 tablespoon of Karo. Then PC for 15 minutes (only!) at 15psi. Let cool and inoculate with your favorite spore solution and then place a band-aid over the inoculate hole and incubate at 84-86F for about 10 days. That's all there is to it. Good luck.
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kjb1891
MycoManiac
Registered: 07/17/02
Posts: 341
Last seen: 12 years, 7 months
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Re: Liquid Culture Tips [Re: MoneyAddyct]
#3306219 - 11/01/04 06:44 PM (19 years, 5 months ago) |
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Thanks for the reply, Money. I've always used polyfill or filter discs. Never used tyvek before. I've always seen a lot of people talking about it on here though. You usually get it from the Post Office?
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kraj
Stranger
Registered: 09/01/04
Posts: 382
Last seen: 3 years, 9 months
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Re: Liquid Culture Tips [Re: kjb1891]
#3306235 - 11/01/04 06:47 PM (19 years, 5 months ago) |
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Yep, the post office has envelopes you just grab
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xistenz
Stranger
Registered: 08/05/04
Posts: 159
Last seen: 18 years, 7 months
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Re: Liquid Culture Tips [Re: kjb1891]
#3306240 - 11/01/04 06:49 PM (19 years, 5 months ago) |
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yeah, grab some of the priority mail envelopes. they're free!
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indigo758
psyentist
Registered: 07/01/03
Posts: 114
Last seen: 17 years, 6 months
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Re: Liquid Culture Tips [Re: xistenz]
#3306865 - 11/01/04 09:08 PM (19 years, 5 months ago) |
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i just got mine in the mail for free, as many as you want if you're a real slacker... 
http://shop.usps.com/cgi-bin/vsbv/postal...jsp?OID=1003543
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please allow me to introduce myself, i'm a man of wealth and taste...
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lewis11
Stranger
Registered: 11/24/04
Posts: 2
Last seen: 19 years, 2 months
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Re: Liquid Culture Tips [Re: kjb1891]
#3415263 - 11/27/04 07:59 AM (19 years, 4 months ago) |
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What is the purpose of the polyfill / filter discs / tyvek ?
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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Re: Liquid Culture Tips [Re: lewis11]
#3415295 - 11/27/04 08:30 AM (19 years, 4 months ago) |
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hmm i oust the working area before cooking,i then lysol the inside of my jars out and lids. i then keep everyhting on paper plates before cooking. i boiled my jars for 35 minutes low temp. i had tvek under lid with coffee filters on top with foil..i trim up the cofee filters so they don't just soak up a bunch of excess water. i have my jars in a cabinet with a dresser 2 heating pads,1 is under the jars with a towel between low setting then another is against the cabinet wall for extra heat in the area. temps stay at 84 to 86 last couple times checking. i also have my jars in a insulted bag for cold hot drinks food forgot to mention it retains heat
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MushroomFriend
I smell a conspiracy!
 
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: indigo758]
#3415298 - 11/27/04 08:32 AM (19 years, 4 months ago) |
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Too bad:
" Orders for shipping supplies to addresses outside the United States cannot be placed online. If you need to make changes to your shipping address, click here "
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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suxs to be in eruope huh?
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: djred]
#3415307 - 11/27/04 08:37 AM (19 years, 4 months ago) |
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lol, does it?
Actually not at all! More freedom in this little country then in all the states and canada together! 
Its only very very crowded...
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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well consider your situation with ordering spores my friend.i could careless about anything else that is or is not. eruope may have more choices for drugs of choice ot be legal but my friend america is the freedom of all the world..so we have stupid laws that say we can't have this nor that..thats ok they can't stop me..from my freedom of choice so in reality we all have freedom .just some have to be careful about the choices they make..
Edited by djred (11/27/04 08:42 AM)
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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Re: Liquid Culture Tips [Re: djred]
#3415317 - 11/27/04 08:45 AM (19 years, 4 months ago) |
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your spore problem was only thing i assumed to be related to living in eruope as being shitty at times. i would love to go to the uk..buy up some vinyl hit some clubs..but hey my karo jars i can see the beginings of growth at the bottoms i can see white patches of like mist coming together..its going good its only been 2 days now..this is its 3rd day ..
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: djred]
#3415334 - 11/27/04 08:57 AM (19 years, 4 months ago) |
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Ordering spores? no problem.
No usa is not the country of freedom as you say. But very off topic so I wont discuss it here.
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: djred]
#3415337 - 11/27/04 08:59 AM (19 years, 4 months ago) |
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Come to Holland some day, UK is prison compared to Holland...
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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oh holland is a place where you can get the options to do what you feel,just checked my karo jars..ouch temps were 93 yeah kinda warm but hopefully not killing heat..gotta think in the summer when it gets real hot ..so everything looks ok i need to do the tnt, going to work on it today.
Edited by djred (11/27/04 09:03 AM)
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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Re: Liquid Culture Tips [Re: djred]
#3415345 - 11/27/04 09:03 AM (19 years, 4 months ago) |
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holland has good trance dj's porducers.
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: djred]
#3415379 - 11/27/04 09:36 AM (19 years, 4 months ago) |
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LOL and the best tulips and northern lights! 
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djred
newbie
Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
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thought it was legal to oreder shrooms in holland.but then why order for hella prices when you can grow.
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: djred]
#3415404 - 11/27/04 09:51 AM (19 years, 4 months ago) |
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me newbe m8 must buy spores etc!
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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Culture Tips [Re: kjb1891]
#3415426 - 11/27/04 10:04 AM (19 years, 4 months ago) |
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1/2 teaspoon light dry malt, 1/2 teaspoon corn sugar to quart water, simmer, filter, add to jar, add lid w/filter, PC 15 minutes, innoc, store at around 82F.
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: agar]
#3415450 - 11/27/04 10:20 AM (19 years, 4 months ago) |
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This is like one teaspoon on a quart of water?
Why? Other recipies say 1 tsp to 100ML?
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4hodmt
AspiringMycologist
Registered: 04/06/04
Posts: 759
Last seen: 9 years, 11 months
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i say. keep the honey content down so that the water is more clear...itll help u see the myc. colonies better. and put a hole on the side of the jar, as u will probably use a lot of karo and the needle dosnt go too far down in the jar. this way u can tip it:).
GL!
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_______
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Actually I'm not from the future, but I did lived there for a while."
- Baby Hitler
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: 4hodmt]
#3415482 - 11/27/04 10:42 AM (19 years, 4 months ago) |
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Is that the only reason, just for visibility to keep honey % low?
Yes thought of other thing, that the needle will have trouble reaching, so I used a tiny jar with only 3/4 inch of air between surface and lid.
My karowater is rather yelloweish, bit pisscolour..
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Peterthinks
(Caulking) gun for hire
Registered: 11/10/04
Posts: 2,379
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I inject about 10 cc of sugar water(1cc molasses to 300 ml water)
inside a testube slant that I have grown over with myc then attach it to a little motor that does about 30 RPM.
I leave a little air bubble inside to stir things up and let it run for 3 days.
The mycelium grow thru the water and some of the PDA dissolves.
Pop it suck it up and inject your whatever.
It covers the seed with liquid food and the colonization is creepy fast.
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Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Koala Koolio
TTAGGGTTAGGGTTAGGGTTAGGG
Registered: 01/07/04
Posts: 7,752
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Re: Liquid Culture Tips [Re: Peterthinks]
#3415713 - 11/27/04 12:03 PM (19 years, 4 months ago) |
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I just use one teaspoon of karo light in 100ml of water. half pint normal jar. after two weeks it looked good, shake once in a while. Trash it up with a needle and suck it up. I inoculated the karo with an agar wedge though, so a pure strain might develop different than just spores. quicker though.
as for holland, any restrictions as to mushroom growing? as in, if the cops find one, as long as theres no evidence that they're being dried and packaged/shipped out of the country, its okay?
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You're not like the others. You like the same things I do. Wax paper, boiled football leather... dog breath. We're not hitch-hiking anymore, we're riding!
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Dont know about exact laws, this year they got more tightassed and now official dried shrooms cant be sold, but they are just in all the smartshops we have in the cities. Online they only sell fresh shrooms now.
To growing I dont know, they are not to paranoid about it, I think it is just like with weed. If you have it small scale it wil be ok, if you are a large commercial dude they might nick your stuff.
When I was in the army I got busted once cause I had gone from duty. MP picked me up at home and I had a joint in my pocket plus 3 or 4 dry grams.
When they found it they said what you want, that we throw it away and 4get about it or that we put it on paper and that you have to answer questions to the military judge. I asked what will be my punishment, 25$ fine the ysaid.
Ok toss the stuff I said. Since I payd 12,5$ for it...
And next day when I ws a free man they gave me my joint back.
Wow what he tasted super! The taste of freedom!
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MadHatR
journeyman
Registered: 10/13/03
Posts: 278
Last seen: 3 years, 6 months
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From what all I read when I lived in Amsterdam is that the laws are in place, but they are soft laws that are not enforced. I never wanted to leave, stupid company needing me back in the states...
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Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.
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MushroomFriend
I smell a conspiracy!
Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days
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Re: Liquid Culture Tips [Re: MadHatR]
#3416180 - 11/27/04 02:47 PM (19 years, 4 months ago) |
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Selling fresh rooms is not illegal, but drying is now official forbidden but hardly enforced.
About growing etc I dont know, I am not paranoid/scared at all. Its no priority.... Just like the 100 of 1000's of weed growers are no priority...
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RRhoads
chemistry student
Registered: 10/26/04
Posts: 1,434
Loc: cahleeforneeah
Last seen: 6 years, 6 months
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Re: Liquid Culture Tips [Re: indigo758]
#3416211 - 11/27/04 03:01 PM (19 years, 4 months ago) |
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Quote:
indigo758 said:
i just got mine in the mail for free, as many as you want if you're a real slacker...
http://shop.usps.com/cgi-bin/vsbv/postal...jsp?OID=1003543
LOL
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