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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
cubes strains mycellium
    #3296085 - 10/30/04 03:24 AM (12 years, 5 months ago)

I wonder if the myc. of every strain of cubensis is rhizomorphic cause I?m having problems getting it with 2 strains, Matias Romero and B+ to be more specific. All I get is slightly rhizomorphic myc. with cottony hues even after doing 8-9 transfers. It only happens with these 2 strains, the others I?m working with show the desired rhizomorphs in a matter of 2 transfers.


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Sr_Setahongo


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 3 months
Trusted Cultivator
Re: cubes strains mycellium [Re: Semilanceata]
    #3296404 - 10/30/04 07:15 AM (12 years, 5 months ago)

Yep it will differ between strains..You should never need to go beyond 2 or 3 transfers..you should have a wonderfully fast strain by then. Are you grabbing the rhizmophoric myc as soon as it grows? If you wait for the dish to colonize then chances are even a little cottony stuff will spread into the rhizo stuff. If you get even a little in your transfer then once again wait it out for the dish to be fully colonized the cottony stuff will have time to grow. As soon as you have a slice of rhizo, grab it.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: cubes strains mycellium [Re: scatmanrav]
    #3296581 - 10/30/04 09:12 AM (12 years, 5 months ago)

That?s what I try to do, but I don?t get the rhizos with these particular strains. Gonna post some pics later on so you can see it better. You know, I?m new at agar work, just 3 months experience. Maybe I can?t identify the right stuff yet  :crazy:
Here a pic of a selected strain, please tell me if this is what I should look for:
Thanks


--------------------
Sr_Setahongo


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 3 months
Trusted Cultivator
Re: cubes strains mycellium [Re: Semilanceata]
    #3298415 - 10/30/04 08:11 PM (12 years, 5 months ago)

Thats exactly it! There will always be some fuzzyness to it. Just how it is. When you see them ropes though..you got it.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: cubes strains mycellium [Re: scatmanrav]
    #3299998 - 10/31/04 03:31 AM (12 years, 5 months ago)

Here is a pic of a petri with MR on it. It?s a first transfer from a plate in which spores were germinated:
These are 3 pieces of the most vigorous myc from the original plate. I don?t know what to select for a transfer. Don?t even know if I should tranfer from this plate and waste another one to get just more cottony myc that is not gonna be used. What do you think?

Thanks for the help man


--------------------
Sr_Setahongo


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Invisiblenoxy
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Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
Re: cubes strains mycellium [Re: Semilanceata]
    #3303866 - 11/01/04 08:55 AM (12 years, 5 months ago)

looks like many strains still in that plate, if its one strain, then its sectoring
you trying to isolate a strain?


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: cubes strains mycellium [Re: noxy]
    #3307557 - 11/02/04 02:15 AM (12 years, 5 months ago)

In that plate there are 3 differents types of mycellium selected from the original plate in which spores were germinated, this way I save petris.
I?m trying to isolate a fast growing rhizo strain but it never appears. I?ll keep trying...

Thank you all


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Sr_Setahongo


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