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OfflineBrady
randomizer
Registered: 09/17/04
Posts: 170
Loc: on a damn island , FL
Last seen: 6 years, 8 months
honey tek h2o2
    #3295664 - 10/30/04 12:33 AM (12 years, 10 months ago)

ok so i got a honey tek that ive injected with 1cc and now im seeing white mycillum at the bottom. I want to add some h2o2 but i was wondering if i had to sterilze syringe or what.. i have an empty syringe and some h2o2. will flame sterilizeing the needle be sufficient or will i need to pressure cook the syringe as well?

B.


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Offlinediscman1
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Registered: 08/24/04
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Re: honey tek h2o2 [Re: Brady]
    #3295750 - 10/30/04 01:00 AM (12 years, 10 months ago)

Why do you want to put H2O2 in there if it is colonizing?


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
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Trusted Cultivator
Re: honey tek h2o2 [Re: discman1]
    #3296325 - 10/30/04 06:31 AM (12 years, 10 months ago)

It would aid to prevent contamination, just the same as you add h202 into your humidifier. Whenever you remove myc theres a chance of exposing the culture to mold spores at that point. If theres h202 in there..the spores will be killed, keeping you clean. This can only be added after germination of myc though so that the h202 doesnt kill the shroom spores too.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 8 months
Trusted Cultivator
Re: honey tek h2o2 [Re: scatmanrav]
    #3296328 - 10/30/04 06:33 AM (12 years, 10 months ago)

Sorry I forgot to answer your question..

I personally would PC it only because it takes a few minutes..15 at 15PSI. You could probably get away with flame sterilizing the needle though if your hell bent on not PCing. Dont hold me to that though..

Inject 5-10cc's for a 100ml culture of straight 3%.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrady
randomizer
Registered: 09/17/04
Posts: 170
Loc: on a damn island , FL
Last seen: 6 years, 8 months
Re: honey tek h2o2 [Re: scatmanrav]
    #3298531 - 10/30/04 08:54 PM (12 years, 10 months ago)

ok... but i think you didnt understand me.

I already injected the honeywater tek. I PCed it , then injected it. I see growth at the bottom of the jar. the honey tek said to then add h2o2, to keep bacteria from starting. So what im saying.. Should i pressure cook the syringe that i intend to use to inject h2o2?


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 12 years, 9 months
Re: honey tek h2o2 [Re: Brady]
    #3299011 - 10/30/04 11:13 PM (12 years, 10 months ago)

Yes, you should sterilize your syringe if you want to be thorough.


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