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Invisiblebluebass
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Registered: 10/28/04
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anti-biotics
    #3290948 - 10/28/04 08:50 PM (13 years, 25 days ago)

I was wondering if you could use anti-biotics for indoor mushroom cultivation?I know that when mushrooms grow outside they develop antibodies to contaminants and they grow.But if u grow them say for ex.
via pf tek, they don't develop these antibodies thus they have a greater possibility of contams.If u can use some kind of anti-biotic[s] is it practical?


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Invisiblenoxy
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Re: anti-biotics [Re: bluebass]
    #3291128 - 10/28/04 09:48 PM (13 years, 24 days ago)

for controlling bacteria with agar culture I add about 1/10 gram of gentamycin sulfate to each liter of media before sterilization
Ive never used it in a PF tek but I suppose it could be done


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OfflinePsilygirl
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Re: anti-biotics [Re: bluebass]
    #3292131 - 10/29/04 02:10 AM (13 years, 24 days ago)

theres plenty of antibiotics out there, i add ampicillin to my agar plates. i have also used getamycin sulfate. both work well.


--------------------
"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


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Offlinehawksapprentice
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Re: anti-biotics [Re: Psilygirl]
    #3292616 - 10/29/04 05:11 AM (13 years, 24 days ago)

If anyone needs anti-biotics i can get em for you, almost anykind you could think of.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

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OfflineCiv
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Re: anti-biotics [Re: noxy]
    #3292681 - 10/29/04 06:19 AM (13 years, 24 days ago)

Quote:

or controlling bacteria with agar culture I add about 1/10 gram of gentamycin sulfate to each liter of media before sterilization




Woot! that works?! I have a bottle of Gentamycin Sulfate, we use to mist our cats if they get an upper resp. infection.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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Offlinedmc_
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Re: anti-biotics [Re: Civ]
    #3297647 - 10/30/04 03:59 PM (13 years, 23 days ago)

Civ said:
Quote:

Woot! that works?! I have a bottle of Gentamycin Sulfate, we use to mist our cats if they get an upper resp. infection.




So I could goto my local grocery store and purchase some Gentamycin Sulfate on the Pet Isle? or vet?


Edited by dmc_ (10/30/04 04:00 PM)


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OfflineCiv
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Re: anti-biotics [Re: dmc_]
    #3297662 - 10/30/04 04:04 PM (13 years, 23 days ago)

Quote:


So I could goto my local grocery store and purchase some Gentamycin Sulfate on the Pet Isle? or vet?




We go to a vet supply store in my town. They have a liquid version of it and then they have a powdered version that is more bang for your buck, but you need a good digi scale to mesure it though. Its also used for cattle and such - so cattle feed stores might carry it also.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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OfflinePsilygirl
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Re: anti-biotics [Re: Civ]
    #3298109 - 10/30/04 06:37 PM (13 years, 23 days ago)

FYI, gentamycin sulfate can be added prior to sterilization, while ampicillin and many other anti-bacterials are heat sensitive and must be added after sterilization and some cooling...

make sure you read up on the info before you do it!


--------------------
"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


Puget Sound Mycological Society


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OfflineCiv
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Re: anti-biotics [Re: Psilygirl]
    #3298247 - 10/30/04 07:15 PM (13 years, 23 days ago)

Quote:

FYI, gentamycin sulfate can be added prior to sterilization, while ampicillin and many other anti-bacterials are heat sensitive and must be added after sterilization and some cooling...

make sure you read up on the info before you do it!




Answered my question before I could even ask.  Thanks.  :thumbup:


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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InvisibleZen Peddler
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Re: anti-biotics [Re: bluebass]
    #3303722 - 11/01/04 07:47 AM (13 years, 21 days ago)

i reckon its often a waste of time to be honest. Unless youve got bacteria - which is USUALLY easy to isolate out anyway - my problem with wild prints is nearly always molds rather than bacteria. And if its strong bacteria the gentamycin is pretty useless....


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OfflineFluxburn
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Re: anti-biotics [Re: Zen Peddler]
    #3874505 - 03/05/05 09:55 PM (12 years, 8 months ago)

Yah seriously someone make some genetically engineered stuff to kill green mold and the black bacteria growing on my tub.


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OfflineRogerRabbitM
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Re: anti-biotics [Re: Fluxburn] * 1
    #3875859 - 03/06/05 04:15 AM (12 years, 8 months ago)

Ok, I've mentioned this before, but been working on it a lot lately and this tek really works. I pick a lot of wild edibles up in the mountains and have been cloning/printing them for domestication. Often, both molds and bacteria are embedded in wild mushrooms. It's normally easier to transfer live mycelium away from molds than from bacteria, which seems to 'hitch-hike' on the myc.

If you'll mix up a batch of antibiotic agar, you can pour the hot agar directly on a petri dish with both mushroom myc and contaminants. Completely cover all the growth in the petri dish with a fresh layer of hot antibiotic agar. The hot agar will 'pasteurize' the contaminants, but the live myc will barely be set back. In a couple of days, the mycelium will grow through the layer of agar and appear on top. At this point, carefully 'shave' a bit of the myc off the top without taking any of the agar. Transfer this to a new dish and you now have a clean culture. The very dirtiest of wild specimens might require a repeat of the above, but this procedure usually gets it on the first go. Try it.


--------------------
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OfflineAeolus1369
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Re: anti-biotics [Re: RogerRabbit]
    #3875971 - 03/06/05 04:49 AM (12 years, 8 months ago)

Sounds great, but at what temperature due you add the antiobiotics to the agar? I've heard that (with the exception of gentamycin), they will break down fairly rapidly at elevated temperatures. I always wait until the agar is about 50 Celsius (rule of thumb: cool enough to hold to your neck without burning yourself) before adding the antibiotics and quickly pouring.

--Aeolus

PS--And to be quite technical, mushrooms do not produce antibodies. Those are a special family of proteins produced only by organisms with a specific type of immune system (e.g. mammals).


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OfflineRogerRabbitM
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Re: anti-biotics [Re: Aeolus1369]
    #3876631 - 03/06/05 11:53 AM (12 years, 8 months ago)

I use the antibiotic agar from fungi.com. It withstands the heat of the pc.


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinescatmanrav
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Re: anti-biotics [Re: RogerRabbit]
    #3882139 - 03/07/05 12:41 PM (12 years, 8 months ago)

Interesting RR!


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflinePsilygirl
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Re: anti-biotics [Re: Aeolus1369]
    #3883664 - 03/07/05 07:24 PM (12 years, 8 months ago)

that is correct, Aeolus


--------------------
"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


Puget Sound Mycological Society


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OfflineWorkmanV
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Re: anti-biotics [Re: Zen Peddler]
    #3887624 - 03/08/05 04:07 PM (12 years, 8 months ago)

I like to use 10 mg of benlate (benomyl) per liter of agar to control molds. It is a fungicide that selectively inhibits sporulating molds while allowing mushroom mycelium to grow. I find it amazing that such a chemical exists and actually works. You really need a good scale though, if you add too much nothing at all will grow. Unfortunately, Dupont pulled it from the market and it is now very difficult to find. See also Carbendazim, the active metabolite of benomyl.


--------------------
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