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Offlinegray1
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Registered: 04/30/01
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Loc: brooklyn
Last seen: 20 years, 5 months
theoretical methodology
    #328685 - 05/29/01 07:38 PM (21 years, 4 months ago)

here's a theoretical methodology flow chart. please comment, especially on the notes in () ------->innoculate 50ml brf/of/suc media with spores, allow ~3 days growth (ambient{room temp}, dark place?) ------->use to innoculate 500ml culture media, recipe in previous post, allow to grow, ambient, dark place, allow colonization (+ antibiotic mixture: amp, kan, tet...?) ------->use to innoculate sterile (3:2) brf/vermiculite in sealed rubbermaid container, store cool dark place, allow colonization ------->crumble onto thin layer (1") of pasteurized mix of peat, vermiculite, fine woodchips/sawdust, manure in equal parts in a foil baking dish, cover with an equal amount of material ------->transfer into plastic storage tub with a 1" layer of moist perlite/vermiculite in bottom, cover with lid ------->allow several days for mycelial network to recover ------->expose to light 20min/day (is this a major risk for contamination when the substrate is already dominated by mycellium) to initiate pinning ------->regulate temp and humidity to encourage maximum fruiting ------->harvest ------->case again for second flush? or just let it go.

c12h16n24ohdmt


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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
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Last seen: 20 years, 6 months
Re: theoretical methodology [Re: gray1]
    #328698 - 05/29/01 08:02 PM (21 years, 4 months ago)

What species/strain?

* Do the Free Spore Thing


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Where there's skill, there's a better way..


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Offlinegray1
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Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 20 years, 5 months
Re: theoretical methodology [Re: egghead]
    #328702 - 05/29/01 08:07 PM (21 years, 4 months ago)

two theoretical experiemnts: b+ and golden teacher

c12h16n24ohdmt


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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 20 years, 6 months
Re: theoretical methodology [Re: gray1]
    #328825 - 05/29/01 11:03 PM (21 years, 4 months ago)

It sounds like you're good to get started. If you're going to put all your eggs in one basket, the anti-biotics might be a good idea. I still don't think I'd add them, though.

The 1" layer at the bottom probably isn't necessary. And I don't think extra light will give you any contam probs. Stamets recommends at least 4 hours per day to induce pinning.

Word is that B+ are found of woodchips. Good luck man..

* Do the Free Spore Thing


--------------------
Where there's skill, there's a better way..


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