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Amazon Shop for: ½ Pint Jars, Pressure Cooker

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Offlineswizzol
journeyman
Registered: 01/29/04
Posts: 155
Last seen: 11 years, 2 months
syringe problem, help!!!
    #3283573 - 10/27/04 11:40 AM (12 years, 1 month ago)

Houston, weve got a problem. My friend called me this morning in shock. He woke up and checked on his jars, they look good by the way. He planned to inoculate them tonight. This is where we got the problem. He pressure cooked his syringes and then used a common syringe tek that he has used with new syringes quite frequently. However, this is the first time with syringes that have been pressure cooked. When he checked them, he found that 7ccs of liquid had been lost. Somehow it leaked out through the night. He now only has 3 cc's in each syringe. A total of 9 cc's. While the remaining is extremely dark, it will have to do. But does anyone know what happened? Has this ever happened to anyone before???


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 8 months
Re: syringe problem, help!!! [Re: swizzol]
    #3283610 - 10/27/04 11:50 AM (12 years, 1 month ago)

Why is he putting syringes in the pressure cooker that still have spores in it?

- Silven


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Offlinecasper7512
Danger
Registered: 09/15/04
Posts: 177
Loc: Shaolin
Last seen: 4 years, 9 months
Re: syringe problem, help!!! [Re: swizzol]
    #3283611 - 10/27/04 11:50 AM (12 years, 1 month ago)

you pcd the syringe with spore in it?


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
Posts: 538
Loc: Cali
Last seen: 9 years, 4 months
Re: syringe problem, help!!! [Re: casper7512]
    #3283634 - 10/27/04 12:00 PM (12 years, 1 month ago)

omg people are freaking crazy! :what:


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OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: syringe problem, help!!! [Re: cougercruiser]
    #3283641 - 10/27/04 12:03 PM (12 years, 1 month ago)

you PCed your spores??? you killed them!!! killer !!!!!!!


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Invisiblemetasin
Stranger
 User Gallery

Registered: 10/12/04
Posts: 972
Re: syringe problem, help!!! [Re: Hamurabi]
    #3283712 - 10/27/04 12:24 PM (12 years, 1 month ago)

:samurai:


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Offlinecasper7512
Danger
Registered: 09/15/04
Posts: 177
Loc: Shaolin
Last seen: 4 years, 9 months
Re: syringe problem, help!!! [Re: metasin]
    #3283763 - 10/27/04 12:38 PM (12 years, 1 month ago)

next time instead of boiling them give them to me


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Offlinededboy
Stranger
Registered: 09/27/04
Posts: 10
Last seen: 11 years, 11 months
Re: syringe problem, help!!! [Re: casper7512]
    #3283811 - 10/27/04 12:48 PM (12 years, 1 month ago)

The poor things.....


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OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 1 day
Re: syringe problem, help!!! [Re: swizzol]
    #3283824 - 10/27/04 12:51 PM (12 years, 1 month ago)

LOL, when you pressure cook the syringes the volume is expandin so leaking into the pan.. So less volume left after you cooked them!

Not to mention now you have sterile syringes! :laugh:"

LOL I thought I was the n00b here! :wink:

Wish your friend good luck,

MF

PS: I notice a lot of of "friends" here! Like "my friend has a pmp with..." S omany friends without internet or is it something else? Like "My friend has herpes are there anonymous medicines available for him? " :grin:


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InvisibleKoala Koolio
TTAGGGTTAGGGTTAGGGTTAGGG

Registered: 01/07/04
Posts: 7,752
Re: syringe problem, help!!! [Re: MushroomFriend]
    #3283847 - 10/27/04 12:55 PM (12 years, 1 month ago)

More like, my friend doesn't want to fully incriminate himself on a PUBLIC message board.

It's not entirely stupid, especially for certain individuals. For many it just isn't a big deal though


--------------------
You're not like the others. You like the same things I do. Wax paper, boiled football leather... dog breath. We're not hitch-hiking anymore, we're riding!


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OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 1 day
Re: syringe problem, help!!! [Re: Koala Koolio]
    #3283865 - 10/27/04 12:59 PM (12 years, 1 month ago)

Lol no not stupid, I was thinking since growing mushrooms might be illegal in some countries that people act as if it is about a friend while in the meantime it is their own mushroom garden... :smile:


--------------------


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Offlineswizzol
journeyman
Registered: 01/29/04
Posts: 155
Last seen: 11 years, 2 months
Re: syringe problem, help!!! [Re: MushroomFriend]
    #3283893 - 10/27/04 01:06 PM (12 years, 1 month ago)

NO, you all got me wrong, he pressure cooked the syringes with water in them, then put the spores in using a print!!!


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InvisibleKoala Koolio
TTAGGGTTAGGGTTAGGGTTAGGG

Registered: 01/07/04
Posts: 7,752
Re: syringe problem, help!!! [Re: swizzol]
    #3283919 - 10/27/04 01:14 PM (12 years, 1 month ago)

were they not cooled all the way? you say the syringe is dark. Is it darker than before the amazing water disappearing act? As in, the spore solution is more concentrated? If so, why not just add more sterilized water? I still have no idea how it happened, unless theres a leak or the plunger was pressed.


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 8 months
Re: syringe problem, help!!! [Re: Koala Koolio]
    #3284017 - 10/27/04 01:40 PM (12 years, 1 month ago)

I guess I'm just getting completely confused by the original post.

Quote:

When he checked them, he found that 7ccs of liquid had been lost. Somehow it leaked out through the night. He now only has 3 cc's in each syringe. A total of 9 cc's. While the remaining is extremely dark, it will have to do. But does anyone know what happened? Has this ever happened to anyone before???




When he checked them? You mean when he checked the water that was put in them in the pressure cooker to sterilize it before the print?

If so, why does he only have 3 ccs of water in the syringe? Didn't he empty that water out and use other sterile water to mix with his print to make a syringe? Why would he use the 3 ccs that were left..

I just don't understand the logic here or I'm misinterpretting information.

- Silven


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: syringe problem, help!!! [Re: Silven]
    #3284086 - 10/27/04 01:57 PM (12 years, 1 month ago)

Basically he is saying that somehow his spore syringe leak. Ignore all the PC stuff.

I have no idea. If your syringe isn't air tight, it will leak. Maybe the PC damaged the plunger?


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Offlineswizzol
journeyman
Registered: 01/29/04
Posts: 155
Last seen: 11 years, 2 months
Re: syringe problem, help!!! [Re: discman1]
    #3284161 - 10/27/04 02:15 PM (12 years, 1 month ago)

thats what i think, but the plunger looks like it has a tight ass seal


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Offlineswizzol
journeyman
Registered: 01/29/04
Posts: 155
Last seen: 11 years, 2 months
Re: syringe problem, help!!! [Re: swizzol]
    #3284196 - 10/27/04 02:24 PM (12 years, 1 month ago)

here was the procedure, he loaded the syringes with presteralized water and then loaded them into a jar and into the pressure cooker, cooked them and a shot glass then waited for them to cool, then made the syringes with a print. then they were left to rehydrate, the spores, and in the morning, the water leaked out...


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OfflineMAGnum
veteran

Registered: 07/08/04
Posts: 2,421
Last seen: 5 years, 10 hours
Re: syringe problem, help!!! [Re: swizzol]
    #3284230 - 10/27/04 02:30 PM (12 years, 1 month ago)

Tell your friend to use the left over water and grow the mushrooms to make syringes again.

It sounds like the syringes got pressed somehow.


--------------------
Agent 727
7


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 8 months
Re: syringe problem, help!!! [Re: swizzol]
    #3284231 - 10/27/04 02:30 PM (12 years, 1 month ago)

Ok, I see.

Is it possible that the pressure cooker dried out the rubber seal inside the syringe?

I think it's safe to assume that something to do with the pressure cooker hurt the syringes regardless of whether it's the plunger or not. I'd just buy a couple new syringes I spose' in this case.

- Silven


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineswizzol
journeyman
Registered: 01/29/04
Posts: 155
Last seen: 11 years, 2 months
Re: syringe problem, help!!! [Re: Silven]
    #3284272 - 10/27/04 02:36 PM (12 years, 1 month ago)

I dont think that the plungers were dried out because there was water in the syringes while in the pressure cooker, I think that maybe it leaked where they attatch to the actual needle. He is just worried about how sterile they will be now with all of the air that has sucked into them as a result of the lost water...


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Amazon Shop for: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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