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Offlinekrazycure
Coastal TripTech

Registered: 08/17/04
Posts: 90
Loc: The edge of reason
Last seen: 10 years, 10 months
70% Iso. Alcohol - Sterilized too well?
    #3283196 - 10/27/04 09:05 AM (12 years, 6 months ago)

I figured I'd start practicing some sterility after an average of 1/4 of my jars were getting contams... finally got some tyvek and alcohol HOPING I could put those days behind me.  :tongue:

Well, after inoculating 10 1/2 pint jars of BRF with Keeper's Creepers and and 6 quart jars of WBS with Gulf Coast, I think the alcohol may have killed every damn spore coming out of the syringe. I simply soaked a paper towel in the 70%, and laid the needle in it whenever I was taping holes or doing something else etc. Now, 6 days later, there is NO growth and ANY jars. I've never had that happen before. They are at perfect incubation temps, and both the BRF and the WBS was PC'd, 30 mins and 60 mins respectively. 

How exactly are you supposed to use this stuff without killing your spores??  :evil: Is it ok to re-inoculate jars that didn't receive any viable spores to germinate? As far as I know the jars are still sterile.  :rolleyes:


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Invisibledobinky
infoseeker
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Registered: 01/30/03
Posts: 2,436
Loc: Wiki, Kentucky
Re: 70% Iso. Alcohol - Sterilized too well? [Re: krazycure]
    #3283202 - 10/27/04 09:10 AM (12 years, 6 months ago)

wipe everything down with it and then give it a sec to evap...or blow on it....


--------------------
Today?s Pig is Tomorrow?s Bacon


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OfflineCyber
Ash
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Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 1 year, 21 days
Re: 70% Iso. Alcohol - Sterilized too well? [Re: krazycure]
    #3283205 - 10/27/04 09:11 AM (12 years, 6 months ago)

Hmmm I lay my needle in a cotton ball soaked in 90% ISO with no problem.


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: 70% Iso. Alcohol - Sterilized too well? [Re: Cyber] * 1
    #3283214 - 10/27/04 09:16 AM (12 years, 6 months ago)

blow on it?  Tons of bacteria in your mouth...  don't know if that's such a good idea. :frown:  maybe use an asperator instead?  Like for cleaning camera lens.

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Offlinekrazycure
Coastal TripTech

Registered: 08/17/04
Posts: 90
Loc: The edge of reason
Last seen: 10 years, 10 months
Re: 70% Iso. Alcohol - Sterilized too well? [Re: Sam1912]
    #3283218 - 10/27/04 09:18 AM (12 years, 6 months ago)

blow on it??? Wouldn't that defeat the purpose of sterilizing, seeing as our mouths are filthy??


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 11 years, 1 month
Re: 70% Iso. Alcohol - Sterilized too well? [Re: krazycure]
    #3283238 - 10/27/04 09:31 AM (12 years, 6 months ago)

Yes blowing on it would probably recontaminate it.

You should wait atleast another 2 days before you reinnoculate IMO just incase you are having bad luck and they are about to start growing.

- Silven


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Invisibledobinky
infoseeker
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Registered: 01/30/03
Posts: 2,436
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Re: 70% Iso. Alcohol - Sterilized too well? [Re: Sam1912]
    #3283297 - 10/27/04 09:55 AM (12 years, 6 months ago)

blowing on it....fanning it in the air....my friend never had a prob and never has contams....find what works for you


oh he said he likes to gargle the iso alk for a minute then he blows on it... :doggystyle:


--------------------
Today?s Pig is Tomorrow?s Bacon


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Offlinekrazycure
Coastal TripTech

Registered: 08/17/04
Posts: 90
Loc: The edge of reason
Last seen: 10 years, 10 months
Re: 70% Iso. Alcohol - Sterilized too well? [Re: Silven]
    #3283301 - 10/27/04 09:57 AM (12 years, 6 months ago)

I guess...but EVERY jar??? I think something went MAJORLY F-U-B-Bed


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 12 years, 5 months
Re: 70% Iso. Alcohol - Sterilized too well? [Re: krazycure]
    #3283322 - 10/27/04 10:04 AM (12 years, 6 months ago)

...

If you just wiped the needle down with an alcohol wipe like everybody else does, nothing on the inside of the syringe would be comming in contact with alcohol.


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 4 years, 4 months
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Re: 70% Iso. Alcohol - Sterilized too well? [Re: discman1]
    #3286023 - 10/27/04 08:21 PM (12 years, 6 months ago)

If you havn't worked with this strain before give it time. It can take longer sometimes. I have a strain going now that took over a week going to show any signs and one still hasn't (as of 3 days ago on day 10, havn't looked yet, will this afternoon) but I'm confident it will.

It's highly unlikly that the alcohol killed it..it evaps very quickly and if it hadn't by the first hole, it would by the rest of the holes. Not to mention like discman said..very little of the inside of the needle is sterilized with the alcohol (a little will get in the tip sometimes). Thats why we flame sterilize as well..

You also shouldn't need to force any air over it. Just wait a second..it only takes a few for 70% iso to evaporate.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisiblegeorge castanzaM
Lord Of The Idiots!
 User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,328
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Re: 70% Iso. Alcohol - Sterilized too well? [Re: scatmanrav]
    #3288186 - 10/28/04 06:59 AM (12 years, 6 months ago)

never ever blow on your grow....:nonono: :rolleyes:


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 4 months
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Re: 70% Iso. Alcohol - Sterilized too well? [Re: george castanza]
    #3288191 - 10/28/04 07:12 AM (12 years, 6 months ago)

You should have a paint mask on anyway when working with your shit so you can't blow on it :wink:


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 12 years, 5 months
Re: 70% Iso. Alcohol - Sterilized too well? [Re: scatmanrav]
    #3288421 - 10/28/04 10:34 AM (12 years, 6 months ago)

I need to get some masks... I always try to hold my breath, or angle it away if I can though...


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 11 years, 1 month
Re: 70% Iso. Alcohol - Sterilized too well? [Re: discman1]
    #3288477 - 10/28/04 10:58 AM (12 years, 6 months ago)

I do the same as you discman when I don't have my medical mask on.

Sometimes I find myself holding it until I feel like I'm going to pass out.  :lol:


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Offlineamyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 3 years, 3 months
Re: 70% Iso. Alcohol - Sterilized too well? [Re: dobinky]
    #3288518 - 10/28/04 11:26 AM (12 years, 6 months ago)

if your friend never had contams, he hasn't any experience with contamination....but lucky non the less


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 4 months
Trusted Cultivator
Re: 70% Iso. Alcohol - Sterilized too well? [Re: amyloid]
    #3290206 - 10/28/04 06:06 PM (12 years, 6 months ago)

They are at hardware stores like 10 for 2 bucks..dont hold your breath :smile:


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHrethic
A Human, Being

Registered: 01/06/04
Posts: 2,397
Loc: Passing through the bulk Flag
Last seen: 10 months, 18 days
Re: 70% Iso. Alcohol - Sterilized too well? [Re: Cyber]
    #3290304 - 10/28/04 06:26 PM (12 years, 6 months ago)

Quote:

Cyber said:
Hmmm I lay my needle in a cotton ball soaked in 90% ISO with no problem.




:thumbup: :thumbup: me too!!


--------------------
Will all the big boomers please unveil, please unveil, please unveil.


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Wild Bird Seed

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