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Yes, mycelial fermentation has been used in the gourmet mushroom industry for quite some time. But, you'll likely not find a spore collection clean enough for liquid culture. I've used both these methods are they work. You can use a syringe with sterile distilled water to scrape mycelium from a petri and suck it up. Then inoculate the liquid culture. You can also sterilize tiny screws in your karo water and add wedges and then shake to create satalite colonies of mycelium.
I prefered the first because it doesn't necessitate shaking liquid in unsealed jars.