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lemon_lw
Stranger
Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
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just got my first contam
#3270085 - 10/24/04 01:09 AM (19 years, 5 months ago) |
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i just checked my jars and 3 out of 8 were contaminated. the strange thing is it was only in my B+ jars at the inoculation site. is that usually the first place to get contams. next, does this mean i have a dirty spore syringe or can i hope for a dirty needle and if it is the spore syringe is there any way to save it.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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noxy
Dr
Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
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Re: just got my first contam [Re: lemon_lw]
#3270105 - 10/24/04 01:14 AM (19 years, 5 months ago) |
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i would guess dirty needle if you didnt flame sterilize it before/between injections what do the contams look like?
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lemon_lw
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Registered: 10/17/04
Posts: 3,622
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Re: just got my first contam [Re: noxy]
#3270127 - 10/24/04 01:18 AM (19 years, 5 months ago) |
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they were a dark green, i already trashed the damn things i was pissed, but your right now that i think of it the very first one i inoculated doesnt have a problem but the three thereafter did. ok so next question how do you clean dirty jars im gonna boil, soak in bleach, then boil again to be safe.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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noxy
Dr
Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
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Re: just got my first contam [Re: lemon_lw]
#3270133 - 10/24/04 01:20 AM (19 years, 5 months ago) |
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you got it
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lemon_lw
Stranger
Registered: 10/17/04
Posts: 3,622
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Last seen: 16 years, 6 months
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Re: just got my first contam [Re: noxy]
#3270143 - 10/24/04 01:22 AM (19 years, 5 months ago) |
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cool thanks noxy for helping me see something so obvious.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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MAGnum
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Registered: 07/08/04
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Re: just got my first contam [Re: lemon_lw]
#3270149 - 10/24/04 01:25 AM (19 years, 5 months ago) |
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Don't be pissed. Contams are a part of nature and don't throw them away, Flush them.
In the sewers mold finds itself a paradise
-------------------- Agent 727 7
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lemon_lw
Stranger
Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
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Re: just got my first contam [Re: MAGnum]
#3270157 - 10/24/04 01:26 AM (19 years, 5 months ago) |
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good perspective magnum, im good now.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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iluan
Keep your stickon the ice.
Registered: 04/24/04
Posts: 2,101
Loc: lookin at crazy in the re...
Last seen: 10 years, 8 months
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Re: just got my first contam [Re: lemon_lw]
#3270251 - 10/24/04 01:50 AM (19 years, 5 months ago) |
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One mistake i made when starting out.. when i had a syringe i didn't have room to suck air up into it. I squeezed off some into a jar then sucked air thru a flame to shake.. big mistake... my first jar was fine, but i musta sucked up some contams when i drew air. If your sure your jars are PC'd good and contam free, then suck the air right back from the jar you first noculate.
-------------------- Remember..if the women don't find ya handsome,they should atleast find ya handy! I do what i can with what i don't have!
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
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Re: just got my first contam [Re: MAGnum]
#3271674 - 10/24/04 02:13 PM (19 years, 5 months ago) |
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Quote:
MAGnum said: Don't be pissed. Contams are a part of nature and don't throw them away, Flush them.
In the sewers mold finds itself a paradise
Molds are a part of nature but us aiding in the cultivation of them (albiet accidental and unwanted) is not a part of nature. Jars should be PCed or injected with bleach before cleaning them to help yourself out killing off the mold so you don't have problems when you dump them. Not to mention, I'll be damned if any open air contams are going in my house ANYWHERE let alone down the toilet in the bathroom which is where I do all my sterile work. If you empty your jars out in the house, your asking to lose your whole grow.
To clean the jars..after dumping them outside, the jars go in a bucket of bleach and soap water I bring down. Then carried up..rinsed out very well..no chunks or spots so they look clean then go through the dishwasher. That thing has to get up and/or over 200 degrees from what I can tell when I open it. Then they end up getting rinsed later right before anything goes in them and then PCed once loaded. I rarly have problems with contams, but they do happen, no matter how sterile you are. The goal is to minimize it happening.
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lemon_lw
Stranger
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Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
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Re: just got my first contam [Re: scatmanrav]
#3271688 - 10/24/04 02:19 PM (19 years, 5 months ago) |
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good thinking iluan,thanks
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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