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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 3 years, 1 month
possible wood-lover tek?
    #3267388 - 10/23/04 12:18 PM (12 years, 8 months ago)

i was thinking......

u take a quart jar, fill it with alder chips, and KARO-style honey water. then u sterilize. then u innoc. with sum azure spores. wouldnt the spores grow a KARO in the fluid, and then efficiently COVER the wood in myc. then just take out, and let the chips sit in the cool till theyre colonized.

would that work?

if so, i think it would be a lot faster than using pieces of spawn/agar.


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
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Re: possible wood-lover tek? [Re: 4hodmt]
    #3268760 - 10/23/04 07:20 PM (12 years, 8 months ago)

FUCKING BUMP!

i wish this stayed in advanced cult. nobody is gonna answer this here.


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
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Re: possible wood-lover tek? [Re: 4hodmt]
    #3268778 - 10/23/04 07:24 PM (12 years, 8 months ago)

Quote:

4hodmt said:
FUCKING BUMP!

i wish this stayed in advanced cult. nobody is gonna answer this here.




adding the karo is not necessary and could invite contams...:thebird:
:lol: :tongue2:


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
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Re: possible wood-lover tek? [Re: george castanza]
    #3268846 - 10/23/04 07:46 PM (12 years, 8 months ago)

hence the sterilization and the wood being in the jar. the KARO would be used to propagate a liquid mycelium culture which would be exposed to every inch of surface of the wood, and probably would seep in, too. this would probably make for a faster colonization rate rather than having chunks of substrate/agar sitting on top the wood. You could let the wood then sit at a temperature colder than contams can not grow in, but myc. can. once the myc. has a nice head start, let it get to a warmer place and let it build up its myc. network more. I just think that this could save a LOT of time for colonization


and why did u hav to give me the finger?!?!


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

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Invisiblegeorge castanzaM
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Re: possible wood-lover tek? [Re: 4hodmt]
    #3268998 - 10/23/04 08:33 PM (12 years, 8 months ago)

just funn' wit ya!
:peace:

so go for it! post back and let us know what happened


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlineforgieboy
consumer ofmushrooms ofvarious types
Registered: 08/31/04
Posts: 9
Loc: australia (vic)
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Re: possible wood-lover tek? [Re: 4hodmt]
    #3274650 - 10/25/04 10:47 AM (12 years, 7 months ago)

Wow, amazing coincidence... I haven't looked on the shroomery in a coupla weeks, and just tonight I was wondering if soaking woodchips in honey water then adding myc would be in any way effective (I'm in Australia, so I'm working with subaeruginosa, but whatever works should work for both hopefully). Anyway I'm going to do some experiments with my sub culture along the same lines. I'll let you all know what the results are if it's in any way fruitful.

And yeah, 4hodmt, go for it!


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OfflineAnnoA
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Re: possible wood-lover tek? [Re: forgieboy]
    #3274696 - 10/25/04 11:04 AM (12 years, 7 months ago)

Better:
1. Prepare grain spawn
2. spawn the grain to sterilized or pasteurized woodchips

Check www.fungifun.org/azurescens


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Offlinescatmanrav
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Registered: 05/08/04
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Re: possible wood-lover tek? [Re: Anno]
    #3275711 - 10/25/04 04:50 PM (12 years, 7 months ago)

You shouldnt soak the wood chips in karo water, thats what george was getting at.

You could most likly do this. I have recently stuffed quarts full of straw and PCed them (straw was soaked and drained well) then knocked them up with 5 cc's of liquid myc and 3 days later..they're probably about 50% colonized. Same sort of thing that people say you shouldnt do.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlineforgieboy
consumer ofmushrooms ofvarious types
Registered: 08/31/04
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Loc: australia (vic)
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Re: possible wood-lover tek? [Re: scatmanrav]
    #3292565 - 10/29/04 04:44 AM (12 years, 7 months ago)

Here's what I did: got some pine bark, pasteurised for bout half an hour, drained to get rid of the filthy brown water, added water again, this time with a large dollop of honey, pasteurised for another 90 minutes, added some small (non-sterile) chunks of subae myc. Well not even 72 hours later, the whole container has very sparse myc growing in it.... it's a strange kind of growth, which would be explained by the fact that there's only a small amount of actual nutrients. It's all the really fine 'hairs' and they are very small at this stage. I'll report back in a few days. As far is I understand honey is very resistant to contams, right? There's no sign whatsoever of contams, but it's early days.

Maybe this could be a starting step for the whole 'beat the clock' methodology, whereby instead of sterilising everything you try and get the myc to colonise fast as hell and beat the contams to it.


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OfflineLeukoism
Dark Canopy View

Registered: 09/18/04
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Re: possible wood-lover tek? [Re: forgieboy]
    #3292579 - 10/29/04 04:52 AM (12 years, 7 months ago)

I dont think honey is resisant to contams at all. Think about this, the mycelium fungus is actually considered a contam to 'normal' people. It is a spore just like mold spres, and honey doesnt have the ability to choose fungus over mold. They are all in the same boat my friend.


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-----Enter the craze untill you have seen!-----

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Offlinescatmanrav
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Registered: 05/08/04
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Re: possible wood-lover tek? [Re: Leukoism]
    #3292676 - 10/29/04 06:11 AM (12 years, 7 months ago)

Absolutly right, honey is NOT resiliant to contams and will actually greatly increase your risk for contams. Its food, how is it going to fend off contams? What you pasturized can fight off contams but if you soak it in honey, your asking for problems.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineforgieboy
consumer ofmushrooms ofvarious types
Registered: 08/31/04
Posts: 9
Loc: australia (vic)
Last seen: 12 years, 4 months
Re: possible wood-lover tek? [Re: scatmanrav]
    #3296163 - 10/30/04 04:12 AM (12 years, 7 months ago)

Firstly - I am well aware that what is food for mould is generally food for myc. The reason I thought (think?) that honey is contam reistant (relatively, anyway) is that I can make honey water myc without sterilisation with minimal contams, just try doing the same thing with any kind of normal sugar..... it smells pretty bad after a couple of days. I can't remember exactly (and sorry but I couldn't be shagged doing the research right now) what I read about honey that implied it was contam resilient, but it may have had something to do with antiobiotics? Not too sure.

If honey takes longer to contam then other nuts, then that makes it 'relatively' contam resilient, does it not? I mean if i did the same thing with coffee, it would be all over by now in the way of contams.....


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
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Re: possible wood-lover tek? [Re: forgieboy]
    #3296418 - 10/30/04 07:26 AM (12 years, 7 months ago)

Coffee is MUCH more complex then a simple sugar. Malt, potato water, water from grain boils, poo runoff, karo, sugar, honey can all be used and contam just as easily as anything else. You can most certainly prepar


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Edited by scatmanrav (10/30/04 07:49 AM)


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Offlineforgieboy
consumer ofmushrooms ofvarious types
Registered: 08/31/04
Posts: 9
Loc: australia (vic)
Last seen: 12 years, 4 months
Re: possible wood-lover tek? [Re: scatmanrav]
    #3296430 - 10/30/04 07:38 AM (12 years, 7 months ago)

Well you may be able to assure me 100% that honey has no antibiotics in it, but try googling 'honey antibiotics'...... there a lot of articles that refer to honey's antiobiotic properties. And a lot of articles about how honey doesn't spoil like most food.


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Offlinescatmanrav
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Re: possible wood-lover tek? [Re: forgieboy]
    #3296489 - 10/30/04 08:03 AM (12 years, 7 months ago)

Well upon looking about I've found talk of it..however I CAN assure you that it honey can definatly get bacterial contams and mold contams..I've had both in it.

The original point of what I said not to use honey for is it will increase your odds of contamination..not hinder it. Just because it has small hints of an antibiotic doesn't mean that's anywhere near enough to fight off any contams from eating it since it is still primarily food.

Yes honey can be left out without rotting but what about something soaked in honey water..try two slices of bread..one soaked in water..one in honeywater and leave it out and see which contams quicker. It would be the honey one..that was my original point.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineforgieboy
consumer ofmushrooms ofvarious types
Registered: 08/31/04
Posts: 9
Loc: australia (vic)
Last seen: 12 years, 4 months
Re: possible wood-lover tek? [Re: scatmanrav]
    #3299553 - 10/31/04 01:52 AM (12 years, 7 months ago)

Yeah I understand what you are saying. I am just saying that if you got two slices of bread, one dipped in sugar water and one in honey water, the sugar water would mould first. Haha we're all agreed but I've had the last say! Only kidding :smile:

As would be expected, the honey-chips did contam, but a good two days after the myc had spread over them. What would happen if, hypothetically, I were to give it all a peroxide rinse? Or maybe gave it a peroxide rinse after the three day 'myc wins' period?

Probably nothing good, but there's always a slim chance that some new way of doing things could be found.


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OfflineGr0wer
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Registered: 09/16/03
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Re: possible wood-lover tek? [Re: forgieboy]
    #3299658 - 10/31/04 01:22 AM (12 years, 7 months ago)

If you want fast colonizing chips just inoc with liquid myc via karo/honey. Or innoc grains then spawn that to chips.


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