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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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OfflineTheGreatButtPaca
Happy Gardner

Registered: 10/19/04
Posts: 57
Loc: KY
Last seen: 13 years, 9 days
Need some experts to help me through my first grow!
    #3258854 - 10/21/04 01:49 AM (13 years, 1 month ago)

Hi all!

I began my first grow Monday 18.  P. Cubensis Cambodian.
  (Was this a good strain?)  
:syringe: I mixed up some subtrate (2 vermiculite/1 ground brown rice/1 water) Filled 12 half pint jars, (Standard Tek)
I put the lids rubber side down but i didn't tighten them all the way.  The syringe holes (4 of them) were taped with masking tape.  The jars were then sterilized in a pressure cooker.(15psi 60min) I tightened all the lids down tight. Once jars were cool to touch I inoculated them with a 12cc syringe. (1cc per jar) and taped the holes back up.
I put them away in dark warm place were they still are today..(Thursday)  now for my questions....

When should I notice a visible change?
Did I screw up by going rubber seal down?
What are the best light conditions for the germination and colonization?

Thanks


--------------------
All Hail!!!..................................420!!!


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OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,047
Loc: El Paso, TX Flag
Last seen: 4 days, 6 hours
Trusted Cultivator
Re: Need some experts to help me through my first grow! [Re: TheGreatButtPaca]
    #3258878 - 10/21/04 01:54 AM (13 years, 1 month ago)

Rubber seal keeps it sealed. Did you use any foil? You shuld always have foil over the lids for PC'ing. Keep em warm and dark, at least 75F and optimaly 85-86F. They should germ in 2-4 days.


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Invisiblenotsloth
Stranger

Registered: 09/10/04
Posts: 50
Re: Need some experts to help me through my first grow! [Re: Gr0wer]
    #3258898 - 10/21/04 01:59 AM (13 years, 1 month ago)

Rubber side down is fine, except your jars are totally sealed (rubber side down and taped holes). I think I'd be inclined to take off the tape and cover with foil, but I'm not sure if you'd be risking contams by using un-PC'd foil and (briefly) exposing your holes to open air.

Personally, I think I'd risk it, but you might want to wait for a few more opinions :smile:


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InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Need some experts to help me through my first grow! [Re: TheGreatButtPaca]
    #3258925 - 10/21/04 02:07 AM (13 years, 1 month ago)

When should I notice a visible change? Within the next two weeks.

Did I screw up by going rubber seal down? You can say I've seen that done many times and it was fine.

What are the best light conditions for the germination and colonization? Germ none. Colonization none.

Remember once you see the myc start to grow tke the tape off the holes. About half way when the jars are 50% done flip them upside down, it allows for co2 to escape easier and you get faster colonization


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,047
Loc: El Paso, TX Flag
Last seen: 4 days, 6 hours
Trusted Cultivator
Re: Need some experts to help me through my first grow! [Re: notsloth]
    #3258926 - 10/21/04 02:08 AM (13 years, 1 month ago)

If your afraid of the foil being dirty, which it should be rather clean off the roll, you can always spray or swipe with some 70% iso.


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OfflineTheGreatButtPaca
Happy Gardner

Registered: 10/19/04
Posts: 57
Loc: KY
Last seen: 13 years, 9 days
Re: Need some experts to help me through my first grow! [Re: TheGreatButtPaca]
    #3258958 - 10/21/04 02:21 AM (13 years, 1 month ago)

Thanks for the suggestions. I think I'll hold out on removing the tape in fear of contaminating the jar. But I will remove the tape and flip the jars once they are about 50% colonized and are pretty stable.

Is 90 degrees better or worst than 75 degrees for colonization?


--------------------
All Hail!!!..................................420!!!


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InvisibleKoala Koolio
TTAGGGTTAGGGTTAGGGTTAGGG

Registered: 01/07/04
Posts: 7,752
Re: Need some experts to help me through my first grow! [Re: TheGreatButtPaca]
    #3259036 - 10/21/04 02:57 AM (13 years, 1 month ago)

I'd say its worse. But.. someone else can confirm/deny


--------------------
You're not like the others. You like the same things I do. Wax paper, boiled football leather... dog breath. We're not hitch-hiking anymore, we're riding!


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Offlinekraj
Stranger
Registered: 09/01/04
Posts: 382
Last seen: 4 years, 8 months
Re: Need some experts to help me through my first grow! [Re: Koala Koolio]
    #3259724 - 10/21/04 10:29 AM (13 years, 1 month ago)

My incubator was running pretty hot for a day or so at 90, i thought maybe it'd take a little bit of water out of the jars. Although it didn't do anything for the jars water-wise, i'm experiencing about half dollar size growth at all the innoc points after 6 days. It's running right about 85 now. I'll let ya know how it goes


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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