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Amazon Shop for: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Vermiculite

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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Syringe Water Injection? Help Newbie **Pictures**
    #3257965 - 10/20/04 10:09 PM (12 years, 1 month ago)

The growth of the mycelium in my PF style 1/2 pint jars seems to be slowing down. It looks like the substrate is becoming dry. I used 2 parts vermiculite to 1 part BRF to 1 part water. Would it be okay to inject water into the substrate with a sterile syringe?

Check out my jars at :
http://img.photobucket.com/albums/v166/bjared2004/DSCF0362.jpg

Also, is this good colonization for 2 weeks after inoculation?


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: actionshroom]
    #3257990 - 10/20/04 10:17 PM (12 years, 1 month ago)

Wow man, that's all you've got after 2 weeks? :frown:

I would also like to know more about this tek, I have some experimental jars that are too dry that I would like to salvage.


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Offlinekraj
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Registered: 09/01/04
Posts: 382
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Re: Syringe Water Injection? Help Newbie **Pictures** [Re: discman1]
    #3258000 - 10/20/04 10:19 PM (12 years, 1 month ago)

Are you using an incubator?


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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: discman1]
    #3258007 - 10/20/04 10:20 PM (12 years, 1 month ago)

Discman, thanks for the encouragement... lol
Is the colonization really that behind?
I havent been able to find images of mycelium growth at the same stage as me.


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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: actionshroom]
    #3258010 - 10/20/04 10:22 PM (12 years, 1 month ago)

No im not using an incubator, the temp is around 70 Fahreinheit.


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Offlinediscman1
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Registered: 08/24/04
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Re: Syringe Water Injection? Help Newbie **Pictures** [Re: actionshroom]
    #3258025 - 10/20/04 10:28 PM (12 years, 1 month ago)

Here are some pics of what my first jars looked like after 7 days. These are Cambodian.





Here's what they looked like 5 days later, a total of 12 days.





Hope that helps.. and I hope someone posts info about water injection. That is why I was asking the proper way to sterilize a syringe the other day.

Edit: That is most likely your problem. Get those temps up to 86?F ASAP!

I noticed a big growth rate improvement just raising my incubator temps from 80 to 86?F.


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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: discman1]
    #3258044 - 10/20/04 10:32 PM (12 years, 1 month ago)

Discman, did you use an incubator?
How long did it take to fully colonize the jars?
Is Cambodian a faster colonizer than B+?
Do you think there is something wrong with my substrate?

PLEASE...  :confused:


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InvisibleSam1912
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Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: discman1]
    #3258047 - 10/20/04 10:32 PM (12 years, 1 month ago)

I keep my jars at 84F. Don't want to warm them up too much you know. Just get them within 82-85F. You'll see remarkable growth.

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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InvisibleSam1912
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Registered: 09/23/04
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Loc: Cali
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: Sam1912]
    #3258050 - 10/20/04 10:34 PM (12 years, 1 month ago)

and oh yeah.. don't inject water. Don't question the water content. I'll be okay. Number one reason for stalled jars is too much water.

Good luck

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: Sam1912]
    #3258061 - 10/20/04 10:37 PM (12 years, 1 month ago)

Thanks Sam, and Discman.

Also, I need some method of incubation w/o a heat pad, or aquarium heater. I cannot store these jars in the open, ie. computer, fridge.


Edited by actionshroom (10/20/04 10:42 PM)


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Offlinediscman1
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Re: Syringe Water Injection? Help Newbie **Pictures** [Re: actionshroom]
    #3258116 - 10/20/04 10:50 PM (12 years, 1 month ago)

Yes, I had my temperatures between 80 and 86?F the whole time. 70?F is much too low, I bet it is your problem.

I'm not sure how Cambodian compares to B+. It was definately much, much faster than my Ecuador jars. This is all I had after 7 days on the Ecuador:



Assuming you mixed it up at the proper ratios, there should be nothing wrong with your substrate. Get those temps up!

My incubator consists of a cardboard box on the top shelf of a large bookcase. My room is warm, and temps up there stay at about 75?F by themselves. To raise the temp to the proper ~85?F, I use a small 18" T8 15W fluorescent bulb. The box is propped up with some books, and the light fixture sits under it on a timer, 1 hour on, 1 hour off.

Temp right now is 82.8?F, and the light should come back on in about 20 minutes.


Edited by discman1 (10/20/04 11:04 PM)


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Invisibleactionshroom
RookieMycologist

Registered: 10/15/04
Posts: 210
Re: Syringe Water Injection? Help Newbie **Pictures** [Re: discman1]
    #3258176 - 10/20/04 11:02 PM (12 years, 1 month ago)

Thanks discman, that seems like a good idea.


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