Psilocybin Extraction with A/B yielding crystals...

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Registered: 07/14/04
Posts: 851

Psilocybin Extraction with A/B yielding crystals...

#3257466 - 10/20/04 05:48 PM (21 years, 6 months ago)

Extraction of psilocybin and psilocin from psychoactive mushrooms

This is a revised copy of a previous write-up about an extraction of the psychedelic alkaloids from psychoactive mushrooms, mainly p. cubensis. The extraction will work for all of the psychedelic mushrooms that contain psilocybin/psilocin. It is an acid/base extraction derived from the DMT extractions that I have read about and performed. The method that I am listing below still has not been attempted by me, so I?d like to note that it is more of a theoretical process waiting on revision until it is finally attempted. The more input I can receive the better the final process and instructions will be. Please feel free to comment.

Overview: The psilocybin is to be extracted from pulverized mushrooms. As the dosages for psilocybin is beyond measuring with my digital scales, I will use 5 grams of dried p cubensis mushrooms. As I know the effect of this amount of mushrooms, I can compare the final extract with the trip from the 5 grams. The alkaloids will be removed from the mushrooms with an acidic solution and defatted with naphtha. After this the solution will be basified and the alkaloids removed with naphtha. The naphtha will be washed with two distilled water washes. The naphtha will be evaporated and the crystals washed one more time with cold distilled water to remove any traces of any basic solutions used in the extraction. The crystals that are left will be consumed and the effects judged to determine the performance of the extraction.

Materials:
Digital pH meter 1000mL Separatory Funnel
Acid (vinegar, muriatic, hydrochloric, sulfuric) Base (baking powder)
Distilled water Three quart Mason jars
Coffee Filters Glass stirring rod
Dried p. cubensis mushrooms VM&P Naptha

Procedure:
1) Prepare the mushrooms for extraction. They should be dried completely. All remnants of the casing layer should be scraped away. Grind the mushrooms in a coffee grinder to a fine powder.
2) Using the digital pH meter prepare 200mL of distilled water and acid to a pH of 2 in a quart Mason jar. You want to stir well when doing this. After you mix this, put it in a hot water bath on the oven until it gets to about 90?F. You?ll want all solvents and naphtha, unless otherwise specified in the instructions, to be at this temperature. Don?t get it higher or you?ll risk destroying precious alkaloids.
3) Pour the mushroom powder into the acidic solution. If you want to mix up 25-50mL more of the acidic solution to rinse out the coffee grinder, that?s fine. Just add it back to the Mason jar with the rest of the stuff. Stir well for 5 minutes, and then stir at 10 minute intervals.
4) After allowing the powder to sit in the warm acidic solution for 45 minutes it?s time to filter it and do another wash. The liquid contains a great percentage of the initial alkaloids found in the mushrooms and it is to be kept. Pour the mixture through a coffee filter into a second Mason jar. Put the powder back in the first Mason jar.
5) Mix up another 200mL of distilled water and acid to a pH of 2. Add this to the Mason jar that contains the mushroom powder. Place that Mason jar back into the warm-water bath on the stovetop.
6) Repeat step 4-5 one more time for a total of three washes. The first one should be 45 minutes while the second and third can be for only 15 minutes each. All of the liquid should be poured into the second Mason jar and saved. All of the alkaloids have now been extracted and the mushroom powder may be discarded.
7) We will now de-fat the acidic solution to ensure a purer finished product. Naphtha is what I will be recommending and using in this procedure. Pour the acidic liquid into a separatory funnel. Add 50mL of warm naphtha to the funnel. Turn the funnel upside down and right side up 10-20 times. Do this gently enough that great deals of bubbles don?t form. You?ll need to release the pressure in the funnel. Do this by turning the funnel so that the stopcock is aimed up and open it. I recommend doing this every 2-3 turns.
8) Allow the layers to settle out now. The bottom layer will be the acidic water solution containing the alkaloids and the top layer will be the naphtha containing the extracted fats.
9) Open the stopcock and save the water layer. The naphtha layer is to be discarded. Repeat the naphtha defatting once more to ensure complete defatting.
10) Now it?s time to basify the solution and get our crystals! The DEA site that I gleaned some of this information from states that too high of a pH can damage the alkaloids that we want to extract. They recommend 8.0-8.5pH be used. Since we want this to be as ?gentle? as possible, we will use baking powder as our base. Prepare 200mL of distilled water and baking soda until the pH is about 8.0. Add to the acidic solution, stirring regularly, until the pH reaches 8.0. The crystals are now ?loose? in the solution and ripe for the plucking!
11) Again we will be using naphtha as our solvent but this time, since the crystals are not dissolved in the liquid, they will migrate to the naphtha layer. Put the basified liquid into the separatory funnel and add 75mL of naphtha. It?s pretty much the same as the defatting, except we want to keep BOTH layers, but keep them separate. Repeat this step 2-3 times saving the naphtha each time and re-extracting with fresh naphtha from the basified solution.
12) Now we have naphtha that has all of the alkaloids in it. The basified solution can be tossed. Just to make sure that we got all of the baking soda out we?ll add some water to the naphtha in the separatory funnel and wash it out again. You can shake the separatory funnel now as hard as you want to, as the layers will separate quite rapidly. Do this twice, tossing the water and keeping the naphtha.
13) Time to evaporate that naphtha and watch our crystals form! Put a fan blowing on the naphtha and some heat underneath if you can. Pyrex dishes are great for extractions because you can scrape the crystals off so easy. Don?t get it hotter than 90?F. Naphtha takes a while to evaporate but I guarantee you that it will do just that.
14) After you evaporate the naphtha you?ll be left with some crystals. Put them in a coffee filter and pour extremely cold water over them. Just 100mL or so will be enough to wash away any parts still there that aren?t desired in the finished product. Scrape the crystals off of the filter and allow them to dry in a dark place.

That should do it! Psilocybin/psilocin crystals. Feedback welcome!

Extras:

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