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Tall ass Redhead
Registered: 07/21/04
Posts: 48
Loc: Lawrence, Ks
Last seen: 13 years, 4 months
How long to wait for print before making syringe?
    #3255917 - 10/20/04 09:05 PM (18 years, 1 month ago)

about 24 hours ago I picked a good sized cap to take a print from. I sterilized a 1/2 pint and all my tools for the job. I put a polyfill breather on the lid of the jar, since I didnt have any tyvek. The cap was nearly the size of the bottom of the jar, and now I have a massive print to make a few syringes with. I am going to take the cap out of the ja rin a few hours. My question is.. Should I go ahead and make the syringe soon, or should I let the spores dry out a bit before? By the way, Im planning on just squirting some sterile water into the jar and sucking it back up through a small syringe size hole thats next to the breather and covered with electrical tape. Should this work alright? I figured this would save me a few steps and less risk of contamination from moving the print around to another container. Thanks for any help

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born to grow
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Registered: 12/14/02
Posts: 6,228
Loc: the rain forests
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Re: How long to wait for print before making syringe? [Re: MittsWaQ]
    #3256266 - 10/20/04 10:23 PM (18 years, 1 month ago)

I wait for them to dry a couple days, IMO I believe their outer coating hardens up a bit keeping them from premature germination. I do this because of a few friends that have had their spores germinate in the syringes in the past, so far so good. :wink:

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Invisiblegeorge castanzaM
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Registered: 10/21/02
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Re: How long to wait for print before making syringe? [Re: hyphae]
    #3257501 - 10/21/04 02:55 AM (18 years, 1 month ago)

on the other hand kramer likes to make his syringes fresh as he can
because the spores don't clump together as much as when the print is dry :thumbup:

either way your good! :tongue2:


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