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 blkhole
Comfortably Numb
Registered: 08/10/04
Posts: 104
Loc: Tinpan Alley
Last seen: 16 years, 2 months
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 Why do spores cost more than syringes? 
#3253081 - 10/20/04 04:46 AM (18 years, 1 month ago) |
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I've been looking around and have been seeing that spore prints cost more than syringes. I would think that syringes would cost more: more practical, cost of syringe. Just wanted to know why.
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With our love, we could save the world, if they only knew...
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Rose
Devil's Advocate
 
Registered: 09/24/03
Posts: 22,518
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 Re: Why do spores cost more than syringes? [Re: blkhole]
#3253083 - 10/20/04 04:46 AM (18 years, 1 month ago) |
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A single spore print can make 10-50 syringes.
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Fiddlesticks.
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blkhole
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Registered: 08/10/04
Posts: 104
Loc: Tinpan Alley
Last seen: 16 years, 2 months
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Re: Why do spores cost more than syringes? [Re: Rose]
#3253115 - 10/20/04 04:54 AM (18 years, 1 month ago) |
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oh, wow...how would I make spore syringe if I got a print?
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With our love, we could save the world, if they only knew...
-George Harrison R.I.P.
http://irevolution.org
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Rose
Devil's Advocate
Registered: 09/24/03
Posts: 22,518
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Re: Why do spores cost more than syringes? [Re: blkhole]
#3253126 - 10/20/04 04:57 AM (18 years, 1 month ago) |
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It is a pretty easy process, but you'd need a syringe. Use the search function... since that question realy isn't very related to the topic of this thread.
Shroomery search is a mycologist's best friend.
I use the shot glass technique.
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Fiddlesticks.
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Anonymous
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Re: Why do spores cost more than syringes? [Re: Rose]
#3253151 - 10/20/04 05:01 AM (18 years, 1 month ago) |
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Bo Selecta!
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blkhole
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Re: Why do spores cost more than syringes? [Re: ]
#3253324 - 10/20/04 05:29 AM (18 years, 1 month ago) |
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Is there any way to inoculate without a syringe?
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With our love, we could save the world, if they only knew...
-George Harrison R.I.P.
http://irevolution.org
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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Re: Why do spores cost more than syringes? [Re: blkhole]
#3253408 - 10/20/04 05:45 AM (18 years, 1 month ago) |
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Nope not really. You can get them cheap from here:
http://ryvmed.com/syringesneedles.aspx
Sometimes pharmacies and feed stores have them locally for like a buck each.
You CAN do it. But it would requie a very sterile enviroment (glove box best) Scrape some spores onto some agar (or a substitue like cardboard or brf paste) and once colonized take wedges and inoculate some jars. Hard to do PF jars this way though and that would just you not only into agar work but grains and casing. Easier to make a syringe. But you can go about it any way you want. All depends on your goals.
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msd9
Stranger
Registered: 07/28/04
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Re: Why do spores cost more than syringes? [Re: scatmanrav]
#3253689 - 10/20/04 06:38 AM (18 years, 1 month ago) |
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How about this - Working in a glovebox, add your spores to some Karo solution. Once you get your mycelium, stick the jar back in the glovebox, open it up and remove mycelium with an eyedropper and use it to inoculate your jars.
Hellarisky, but maybe possible if you sterilize all your tools and work in a glovebox. The advantage is it avoids the use of agar and other media. Karo jars are a pretty well accepted and proven way of growing mycelia that don't seem to need the super-sterile conditions of agar.
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 george castanza
Lord Of The Idiots!
Registered: 10/21/02
Posts: 8,534
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 Re: Why do spores cost more than syringes? [Re: msd9]
#3254221 - 10/20/04 08:26 AM (18 years, 1 month ago) |
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Quote:
msd9 said:
How about this - Working in a glovebox, add your spores to some Karo solution. Once you get your mycelium, stick the jar back in the glovebox, open it up and remove mycelium with an eyedropper and use it to inoculate your jars.
Hellarisky, but maybe possible if you sterilize all your tools and work in a glovebox. The advantage is it avoids the use of agar and other media. Karo jars are a pretty well accepted and proven way of growing mycelia that don't seem to need the super-sterile conditions of agar.
if your growing mushrooms at some point you will have to be "super sterile" or as i like to say, sterile 
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KRAMER CAKES
  
 
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scatmanrav
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Re: Why do spores cost more than syringes? [Re: george castanza]
#3254797 - 10/20/04 10:54 AM (18 years, 1 month ago) |
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Karo is just as easily contamed as agar. Hell they're basically the exact same thing, just one has agar agar in it to make it a gelatin type substance. The reason liquid cultures are less likly to contam is you usually can just inject through a filter. Only one small hole when using it for contams to get in through. With agar you open the top to put spores on and then again to cut wedges and remove. And then you can't inject into your jars so you must remove the lid to them. Liquid cultures are only more "contam resistent" because you use a syringe throughout the entire process and no lids come off. Doing it the way you said would remove that contam resistant part of liquid cultures. Not that it wouldn't work..just as well as agar. But no better. Sterility (glovebox) is still needed.
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msd9
Stranger
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Re: Why do spores cost more than syringes? [Re: scatmanrav]
#3256953 - 10/21/04 01:05 AM (18 years, 1 month ago) |
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I remember seeing some pics in Stamets that showed agar in petri dishes where the contams had obviously come in from the join in the petri's. It would also reduce that possibility.
Regardless, I had always heard that agar work required substantially more sterile procedures than other work. Perhaps I was misinformed.
Of one thing I AM pretty sure - Karo is a lot easier to get than agar, and cheaper. It would be my choice in this case.
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blkhole
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Registered: 08/10/04
Posts: 104
Loc: Tinpan Alley
Last seen: 16 years, 2 months
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Re: Why do spores cost more than syringes? [Re: msd9]
#3257305 - 10/21/04 02:17 AM (18 years, 1 month ago) |
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I guess that although it's possible to do it without the syringes, it won't really be worth it due to the contam risks. I guess I'll just suck it up and buy some. I just hope no one around the house opens my package, they might take it the wrong way.
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With our love, we could save the world, if they only knew...
-George Harrison R.I.P.
http://irevolution.org
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discman1
journeyman
Registered: 08/25/04
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Re: Why do spores cost more than syringes? [Re: blkhole]
#3258201 - 10/21/04 06:09 AM (18 years, 1 month ago) |
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Does anyone know somewhere to buy syringes... in less quantity than 100? :P
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Gr0wer
always improving
Registered: 09/17/03
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Re: Why do spores cost more than syringes? [Re: discman1]
#3258282 - 10/21/04 06:25 AM (18 years, 1 month ago) |
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Yea they have 20cc's in a 50 pack for $8. Its nice opening up a clean new syinge when you need one. You can re steralize them by boiling or PC'ing wrapped in foil for 30 min.
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scatmanrav
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Re: Why do spores cost more than syringes? [Re: Gr0wer]
#3258517 - 10/21/04 07:13 AM (18 years, 1 month ago) |
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Yep what he said. Just get the 50. Its 8 bucks for christ sakes. I promise if you can find something like a 10 pack..they'll be more then 10 bucks..go with that site. Unless you can find them at a feed store or pharmacy like I suggested. Like a buck each there..
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george castanza
Lord Of The Idiots!
Registered: 10/21/02
Posts: 8,534
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Re: Why do spores cost more than syringes? [Re: scatmanrav]
#3261496 - 10/22/04 02:47 AM (18 years, 1 month ago) |
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i buy kramer's for him at a feed store ... had to go about 35 miles to get the 1 1/2" needles though...and they are not BD
does anyone know where to get the BD rigs?
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KRAMER CAKES
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xistenz
Stranger
Registered: 08/05/04
Posts: 159
Last seen: 17 years, 3 months
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Re: Why do spores cost more than syringes? [Re: george castanza]
#3261604 - 10/22/04 03:14 AM (18 years, 1 month ago) |
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i bought my BD's at a local pharmacy. they kinda looked at me funny when i asked, i told them i was shooting a horror film and needed some props. they were like a buck a piece. if you can't find them at a pharmacy i'd say just order them from one of the sponsors. i think ralphster sells empty BD's.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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Re: Why do spores cost more than syringes? [Re: xistenz]
#3262150 - 10/22/04 05:15 AM (18 years, 1 month ago) |
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Cheapest place I've seen BD's is ralphstersspores.com He's got a place for empty syringes..two brands..BD is one.
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