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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflinePlowKing
enthusiast

Registered: 11/04/00
Posts: 215
Loc: 345Rotting Street, Hell
Last seen: 15 years, 2 months
Day 8
    #325259 - 05/24/01 06:07 PM (15 years, 6 months ago)

It's been 8 days since the spores came in contact with the substrate. There was enough for 7, 1/2 pint jars. So far 6 show absolutely no signs of growth. 1 Jar has a small little patch of mycelium growing on it. Is there anything I can do to help them out? i got em in a rubbermaid container in my closet with a nice warm blanked covering them.



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OfflineAnnoA
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Re: Day 8 [Re: PlowKing]
    #325264 - 05/24/01 06:17 PM (15 years, 6 months ago)

Give it time. If you don?t see any contamination, at least you worked well and the syringe was clean.
What is the temperature?

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OfflinePlowKing
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Registered: 11/04/00
Posts: 215
Loc: 345Rotting Street, Hell
Last seen: 15 years, 2 months
Re: Day 8 [Re: Anno]
    #325293 - 05/24/01 06:46 PM (15 years, 6 months ago)

temp is about 80 ?F



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Invisiblesynaptic
newbie
Registered: 05/23/01
Posts: 36
Re: Day 8 [Re: PlowKing]
    #325299 - 05/24/01 06:53 PM (15 years, 6 months ago)

How long did you wait for the jars to cool before innoculating them? What substrate formula did you use? Where'd you get your syringe and did you shake it well before innoculation?

Was your substrate really wet? I had some jars start colonizing then stagnate because I tamped the substrate and made it a little too wet. Removing the tape, flipping the jars upside down, and loosening the lid for a day or two helped them along.




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Offlinecbee
journeyman

Registered: 04/26/01
Posts: 49
Last seen: 14 years, 8 months
Re: Day 8 [Re: synaptic]
    #325418 - 05/24/01 09:08 PM (15 years, 6 months ago)

if say you did kill the spores by not lettin them cool long enough, could u just innoc those jars again now that they are cooled or do the killed spores wreck shit ?



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OfflinePlowKing
enthusiast

Registered: 11/04/00
Posts: 215
Loc: 345Rotting Street, Hell
Last seen: 15 years, 2 months
Re: Day 8 [Re: cbee]
    #325601 - 05/24/01 11:21 PM (15 years, 6 months ago)

The substrate is brf / verm. I made my syringe from a print,
The substrate was perfect moisture ( felt just like a nice fluffy cake) ( made / steralized 12 jars total but only had enought innoculat for 7 jars, so I had to ditch the rest. and yes i shook the syringe very very well before i innoculated. I think the problem probably lies in the fact that I didn't flame steralize the needle of they syringe each time I put it in a new hole of each jar....



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Invisibleoscill8
*bondage fairy*
Registered: 02/27/01
Posts: 1,215
Re: Day 8 [Re: PlowKing]
    #325613 - 05/24/01 11:44 PM (15 years, 6 months ago)

plow- you would flame sterilize a needle to cut down on contams, not to facilitate growth in the jars... if you saw contams, you could point to not flame sterilizing (which, btw could kill any spores inside the needle with heat) but for slow growth... it wouldnt be the cause. just give it time. keeping the jars too hot can slow growth as well, so you may want to double check the temps you quote. any funky smells coming from your poked holes? (curious, sometimes when HQ's jars get too hot, they can start smelling like *cooking* or something, though they dont spoil, she knows the temps are off if she smells anything strong coming from the jars).

cbee- you wouldnt want to reinnoc jars that have failed. its just easier in the long run to remake jars than to innoc and (potentially) fail. (you dont know why the jars failed in the 1st place- perhaps there was something wrong w/ them, you dont want competing strains of mycelia in the jar for fear of slower growth due to competition, excess water from 2 innocs could slow growth, brf/verm is pretty inexpensive to come by and the time lost is negligible when compared to the amounts of time youll eventually devote to the hobby... just a few reasons why you shouldnt).



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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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