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OfflineSpagg
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Question
    #3251459 - 10/19/04 02:30 PM (12 years, 6 months ago)

My first set of jars was contaminated with green mold so i took extra care to be sterile second time around. I also made a control jar with the substrate not inoculated with spores. My jars turned green and wet once again but my control has no mold or growth of any kind, indicating it is clean? Is it safe to say that my syringe of spores was contaminated somehow? (btw i flammed the tip before inoculating, and let it cool)


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OfflineAnnoA
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Re: Question [Re: Spagg]
    #3252507 - 10/19/04 07:00 PM (12 years, 6 months ago)

Either:
- the syringe needle
- the syringe contents
- the air that entered at the time of inoculation.

Did the contamination start at inoculation points?


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OfflineSpagg
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Re: Question [Re: Anno]
    #3253599 - 10/19/04 11:20 PM (12 years, 6 months ago)

Yes that contam did start at inoculation points


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OfflineAnnoA
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Re: Question [Re: Spagg]
    #3254568 - 10/20/04 02:50 AM (12 years, 6 months ago)

If it started the whole lenght of the jar where the dropt went down, then the syringe content is contaminated.


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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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