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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Rye Grain, Vermiculite

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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Newbie experiment.
    #3251358 - 10/19/04 02:00 PM (12 years, 1 month ago)

'Well I finally got my syringe. Here's a run down of what I'm going to do.
10 jars done PF Tek style
4 of those jars will use the normal BRF
4 others will use Rye flower instead of BRF
2 will have a mix of rye and BRF BUT will also use a weak coffee mixture instead of plain water.

At 3 days(7pm tue) I'll do my first check to see if anything is growing. I've taken rediculous actions to keep everything sterile. I went though 2 1/2 bottles of rubbing alcohol by spraying everything within 10ft of my work space every few minutes. I've got the jars at a temp between 83 and 86 degrees in a dark box. I will be using a casing meathod with some of my cakes but I haven't figured out how I want to do that yet. I will also be cold shocking/dunking each case. 2 of the cakes will be dunked in a weak coffee mixture. I don\'t know if I want to use the cakes that already used coffee or not yet. Of course my plan will change a bit do to any contams. I hope to give a few more options to newbies to get bigger flushes.'


--------------------
Flashbacks are God's way of saying, "This one's on me".


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: ymhrswrider]
    #3255622 - 10/20/04 12:49 PM (12 years, 1 month ago)

Here's an update.
I checked 6 jars last night at 7pm. 2 rye, 2 BRF, and the 2 mixed with coffee. The BRF both had about dime sized mycelium in all of the inoc spots.
Now, on the other hand, the rye cakes and coffee cakes showed quite a bit more growth. All of the inoc spots had almost a quarter to pinky sized strip of mycelium. Now weather this extra growth in the coffee jars was do to the coffee and not the rye flower mixed in is up to debate. I will check the jars again at day 6. By the way, rye flower was about half the price of BRF.


--------------------
Flashbacks are God's way of saying, "This one's on me".


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
Posts: 538
Loc: Cali
Last seen: 9 years, 4 months
Re: Newbie experiment. [Re: ymhrswrider]
    #3255789 - 10/20/04 01:35 PM (12 years, 1 month ago)

ok, so your saying that you innculated on 10/19/04 and now it is 10/20/04 and you already have a dime siezed mycelium growing in your jars! thats fucking crazy, its been three days and i have like two spots on all 8 of my jars :shocked:


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
Posts: 538
Loc: Cali
Last seen: 9 years, 4 months
Re: Newbie experiment. [Re: cougercruiser]
    #3255904 - 10/20/04 02:01 PM (12 years, 1 month ago)

also i have 6oz glasses which where shot up with the b+ race, i think it should take a shorter time to colonize.what is the race your growing?


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: cougercruiser]
    #3256587 - 10/20/04 04:55 PM (12 years, 1 month ago)

I'm doing Brazilians in pint jars. I was pretty suprised after reading how long it seems to take some people. I made sure the cake was pretty fluffy and not too packed down. I've also kept my hands off the jars completely so that the temp stays at a constant temp. I guess this is why it's working so well. I'm telling you though, the rye flower has at least twice as much myc growing so far.

Oh wait, I just read your post again cougercruiser. I inoculated on Sat 16th at 7 pm. The website was running an update when I tried to post and I had to come back later resend it. It was after 3 days that I saw that growth.


--------------------
Flashbacks are God's way of saying, "This one's on me".


Edited by ymhrswrider (10/20/04 07:07 PM)


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: cougercruiser]
    #3258404 - 10/20/04 11:54 PM (12 years, 1 month ago)

One more update. I checked all the jars again at 4 days because I was bored. The rye jars are holding steady at about twice the growth as the BRF. But get this, the jars that had the coffee mixture are showing about 10% more growth than the rye jars. There are some good sized fingers of myc digging through the cake. I wish I had a camera to show what's going on. I'll keep updating.


--------------------
Flashbacks are God's way of saying, "This one's on me".


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Offlinemebesideme
o-bliminal
Male
Registered: 08/19/04
Posts: 177
Last seen: 5 years, 9 months
Re: Newbie experiment. [Re: ymhrswrider]
    #3258859 - 10/21/04 01:50 AM (12 years, 1 month ago)

More info on your "weak coffee mixture"? Like what exactly is it? Directions for making?


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Doot de dooh.... yep yep yep. A signature and stuff...


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
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Re: Newbie experiment. [Re: mebesideme]
    #3259061 - 10/21/04 03:05 AM (12 years, 1 month ago)

It is bad scientific etiquette to "combine" experiments, because then you do not know which method produced the results.

You should've done regular BRF cakes, rye cakes, and then the mixed BRF and rye, and then doubled the experiment, only with coffee in the BRF, rye, and BRF mix to compare to the "controls".

Let us know how it goes, though! :laugh:


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Newbie experiment. [Re: discman1]
    #3259401 - 10/21/04 06:25 AM (12 years, 1 month ago)

It's a good starting point. There's enough of us to try to duplicate an aspect of this experiment. And besides, you shouldn't believe the results as "facts" until it's been duplicated. Still, a good place to find information. 3days and dime size sounds like about right for good germination and growth from MSI.

And i don't think there's anything new being tested here. I hate to break the party, but these experiments have been... well.. I'll let you have your fun. But... One thing I'm curious about... if you get the coffee one to fruit, let us know if it taste like coffee, okay?

Good luck! Monster mushie wishes!

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
Posts: 538
Loc: Cali
Last seen: 9 years, 4 months
Re: Newbie experiment. [Re: Sam1912]
    #3260089 - 10/21/04 12:49 PM (12 years, 1 month ago)

oh, ok


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: cougercruiser]
    #3260131 - 10/21/04 01:00 PM (12 years, 1 month ago)

God you guys are downers. I know these experiments have been done and I know I don't have a large enough community to call any of these results rock steady. I just couldn't find any clear cut answers to what easy to follow options there were besides the BRF PF tek. This is the first time I've ever tried this, so I think I'm doing ok for my first grow with no contams. Hopefully after this other newbies will have the option of trying BRF or Rye for a higher yeild or rye and coffee for an even higher yeild. That way, the ones of us that aren't really interested in paying $100 for PC, thermometers, heaters, ect. will have some other options. Sorry about the rant. I guess what I'm trying to say is:
NO DUH.


--------------------
Flashbacks are God's way of saying, "This one's on me".


Edited by ymhrswrider (10/21/04 01:02 PM)


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: ymhrswrider]
    #3260153 - 10/21/04 01:07 PM (12 years, 1 month ago)

Oh, to answer the question about the coffee strength. The amount of water I needed to fill up my 2 jars was 1 cup. I drink pretty strong coffee so I added a little more than a shot glass worth of coffee.


--------------------
Flashbacks are God's way of saying, "This one's on me".


Edited by ymhrswrider (10/21/04 01:09 PM)


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Newbie experiment. [Re: ymhrswrider]
    #3260161 - 10/21/04 01:09 PM (12 years, 1 month ago)

I'm not knocking... hell, I'm all for safe and productive experiments. Hell, I'm even for some unsafe and unproductive experiments. Like I said, it'll be fun for you. And it's definitely going to reinforce the knowledge pool. I was just trying to get some negative energy out. maybe I came out wrong!

Keep us updated. And don't forget to let me know if it taste like coffee, okay?

Monster mushie wishies for ya, too!

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: Sam1912]
    #3260173 - 10/21/04 01:13 PM (12 years, 1 month ago)

LOL no worries man. I'll let you know how they taste. I can't wait till it's all done. My buddies and I searched high and low for mushies for over a year with no luck. Now for about the same price as a bag for the setup I will have as much as I need. Sweet!
Quick question, do whole cakes pin much faster than if they were cased?


--------------------
Flashbacks are God's way of saying, "This one's on me".


Edited by ymhrswrider (10/21/04 01:15 PM)


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 8 months
Re: Newbie experiment. [Re: ymhrswrider]
    #3260212 - 10/21/04 01:24 PM (12 years, 1 month ago)

It's good to see someone else experimenting with coffee.

I also did an experiment with coffee and my jars are just getting to full colonization now. Here is what I did and my results so far:

Mixed up 12 jars of substrate using the PF-Tek for Simple Minds.

6 of the jars used 100% water mixed with the vermiculite and BRF.
2 jars were equadorian, and 4 were Thai Super Strain.

6 of the jars used 50% water / 50% coffee mixed with the verm and BRF.
2 jars were equadorian, and 4 were Thai Super Strain.
* Coffee was brewed according to directions on maxwell house can

After doing this I had began incubation.

4 days after inoculation I checked my jars and it seemed that about all jars were equal on growth, the only four bare were the equadorians, coffee and water.

Skip ahead to 14 days after inoculation.

Coffee sets:

Thai Super Strains were 5 still colonizing, 1 complete.
All jars were around 80 - 90% colonized.

Equadorians had been showing growth and were only around 50% colonized

Water sets:
All Thai showed growth - most were around 70% - 80% colonized.
Both EQs showed growth - one was noted at 30% colonization, the other at 50


Skip ahead to today (20 days after inoculation)

Coffee Set:


All Thai Strain has been dunked and birthed. Last one was completed on day 18.

Equadorian is still only around 80 - 90% colonized.

Water Sets:
One water Thai has been dunked and birthed on day 19.
Two more will be dunked today and birthed tomorrow.

The last Thai jars are around 98% colonized. (Two days to go I think)

Equadorians are only around 80% colonized, one is probably only 65% because it seems one inoculation point didn't colonize.


RESULTS:

So far it seems that coffee does infact increase colonization times, according to my results. All controls were in place. Jars were incubated at a constant 83 - 86 degrees F.

I will be using coffee again in my next batch and have more water control jars as well. I am curious to try using coffee with rye grain.

- Silven


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: Silven]
    #3267612 - 10/23/04 01:54 PM (12 years, 1 month ago)

Alright, I checked them again last night on day 6. The BRF is still taking it's sweet time. The coffee mix and rye jars are too close to tell. They all look like they are about 40% colonized. I'll check again on day 9.


--------------------
Flashbacks are God's way of saying, "This one's on me".


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Offlineymhrswrider
Butthead

Registered: 08/04/04
Posts: 337
Last seen: 10 years, 11 days
Re: Newbie experiment. [Re: ymhrswrider]
    #3279847 - 10/26/04 04:22 PM (12 years, 1 month ago)

Day 9
Here's a quick update and my conclusion. I know this is nowhere near scientific enough for any concrete results, but the outcome is still pretty convincing.
The 2 jars that had the coffee mixture are neck and neck at about 95% done. I expect to be able to birth one of them tomorrow morning.
The 4 jars with the rye flower are coming up second with about 75% growth.
The BRF jars are still taking their sweet ass time and are anywhere form 30-60% done.
From now on I am sitcking with the coffee mixture. It has kicked all the other jars asses. Next couple of jars I do are going to be similar to the coffee mixture but with nutritional yeast added. I am also going to inoculate with the honey water myc. I want to see if I can get colonization times down to close the whole grains.


--------------------
Flashbacks are God's way of saying, "This one's on me".


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Rye Grain, Vermiculite

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