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OfflineMikol
tripper
Registered: 09/07/04
Posts: 47
Last seen: 17 years, 7 months
Boiling pf jars and stacking
    #3246790 - 10/12/04 10:32 AM (19 years, 3 months ago)

Here's the deal:
Pf tek
Boiled jars (no PC) for 1.5 hours
1/2 pt jars inoculated w/ GT
First 10 jars inoculated and 9 out of 10 colonized perfectly. I lost one due to contams. I did as much sterilization as I could just like EVERYTHING on this site says.

2nd batch, 3 weeks later.
Same procedures except 20 jars shot up with colorado and TC.
Almost all of the CO jars are getting contams.

So I've done some thinking about why this is happening and here's my hypothesis. On the first batch with the GTs, I had one jar stacked on top of others in one of the pots and it wasn't half submerged in the water like all the others. And with the second 'batch' almost half of the jars where stacked and not halfway submerged in the water. I think maybe this didn't sterilize these top jars enough and therefore ended up getting contaminated.

These are first timers and I don't want to blame it on a bad syringe because I know the shroomery's venders are reliable. Plus, being a first time (even though hours and hours of reading on the subject has been done), there could've been some error when innoculating.

What do you think? Are the top jars not getting 'cooked' enough? Any help/comments would be really nice. This does tempt me to go out and buy a pressure cooker today though. hah
thanks


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OfflineMikol
tripper
Registered: 09/07/04
Posts: 47
Last seen: 17 years, 7 months
Re: Boiling pf jars and stacking [Re: Silven]
    #3246901 - 10/12/04 11:09 AM (19 years, 3 months ago)

Thanks for the responses. I don't pack the substrate mixture hardly at all. And to answer your question oddiseus, the pots are in good working order and don't really let the steam out. I mean it's not a PC, but it does keep steam in pretty well.

I think I'll just make sure not to stack them when not using a PC like I've been doing. Seemed to work great the first time.


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OfflineMikol
tripper
Registered: 09/07/04
Posts: 47
Last seen: 17 years, 7 months
Re: Boiling pf jars and stacking [Re: Sam1912]
    #3247454 - 10/12/04 01:37 PM (19 years, 3 months ago)

Thanks, you guys are awesome here at the shroomery.


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